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BMS antibiotics and agar > Agar > Flashcards

Agar Flashcards

learn what organisms look like and what the agar content is

1
Q

MSA contents

A

Mannitol Salt Agar
~mannitol fermented by SAUR - yellow
~ salt used bc SAUR halophilic

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2
Q

MSA target organism

A

SAUR - yellow cols

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3
Q

CCDA contents and incubation settings

A

Charcoal cefoperazone deoxycholate, no blood

microaerophilic conditions, 41C

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4
Q

CCDA target organism

A

Campylobacter

metallic grey/green appearance

C coli and C jejuni isolated better at 41C

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5
Q

CLED contents

A

Cysteine- supports some colis
Lactose - differentiate LFs
Electrolyte deficient - reduce proteus swarms

indicator is bromomethyl blue

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6
Q

XLD contents

A

Xylose - shigella = non-fermentor

Lysine - Salmonella ferment xylose quick and move onto lysine

Deoxycholate - reduce Gram pos orgs

Sodium thiosulphate and ferric = H2S production

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7
Q

XLD - appearance of shigella

A

clear colonies

no Xylose fermentation so NO YELLOW

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8
Q

XLD - appearance of Salmonella

A

NO YELLOW - ferments xylose and lysine which reverts pH

usually black centres due to H2S production

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9
Q

GVPC contents + incubation conditions

A

Glycine
Vancomycin
Polymixin
Cyclohexamide

Charcoal to neutralise toxins
10 day incubation, moist env

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10
Q

GVPC target organism

A

Legionella

ground glass grey colonies

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11
Q

BCEP contents

A

peptones and sugars to support Burk’s
crystal violet, polymixin B, gentamicin, ticarcillin - SELECTIVE agents

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12
Q

BCEP target organism

A

Burkholderia species

Pink colour

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13
Q

CAP contents

A

Colistin - reduce GNB
Aztreonam - reduce proteus

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14
Q

PCFC contents

A

Pseudomonas selective agar with
Cetrimide
Fusidic Acid
Cefaloridine

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15
Q

PCFC target organism

A

Pseudomonas - green

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16
Q

CIN contents

A

Cefsulodin
Igasin
Novobiocin

Mannitol

17
Q

CIN target organism

A

Yersinia enterocolitica

Bullseye with pink centres - mannitol fermentation

18
Q

TCBS contents

A

Thiosulphate Citrate
Bile Salts
Sucrose

19
Q

TCBS target organisms

A

Vibrio cholera - yellow large cols (ferment sucrose)

V parahaemoliticus - green large cols

20
Q

CT - SMAC contents

A

Cefixime - reduce proteus
Tellurite - reduce colis
Sorbitol Maconkey Agar

21
Q

CT-SMAC target organisms

A

VTEC/STEC are MOSTLY non-sorbitol fermenters and produce clear cols

22
Q

SAB pH effect

A

acidic pH reduces bacterial growth

BMS antibiotics and agar (3 decks)

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