Bacteria Flashcards
Identify the main shapes of bacteria (morphology)
- Coccus, Bacillus
- Can range in size from visible by the human eye to X-ray crystallography
Strepto vs Staphyl
Chain vs Cluster
Gram +ve bacteria traits
- thick cell wall
- stains blue with gram stain
- no outer membrane, maybe s-layer
- thick peptidoglycan with a pentagly bridge between peptidoglycan
glycocyl chains
-teichoic acids between peptidoglycan
Glycocalyces
- do not confer significant structural strength
- poly saccharide layer
Capsules or Slime layer
Capsules
tightly attached, tight matrix, visible if treated with India ink, found in all bacteria
Slime layer
- loosely attached, easily deformed layer that assists in sticking to surfaces,
- plays a role in biofilm formation
- can act as a virulence factor cause it protects the bacteria from phagocytosis
- prevents dehydration/desiccation
S-layer
Protective layer found in FREE LIVING (not lab grown) gram +, - and archaea with a crystalline layer consisting of proteins or glycoproteins, may contribute to cell shape and help protect the cell from osmotic stress
Gram -ve Cell wall
- thin cell wall
- just a peptide crosslink between 2 peptidoglycan
-lipopolysaccharides
lipid A: endotoxin - lipoprotein from outer mb and peptidoglycan
lysozyme
can destroy peptidoglycan
enzymes cleace glycoside bond between sugars
found in human secretions major defence against infection
gram -ve traits
- thin cell wall
-stains pink - LPS
-lipoprotein
outermembrane
gram +ve cell wall
-thick cell wall
-peptidoglycan with pentagly bridge
peptideglycan + teichoic acids
teichoic acids
- glycopolymer
helps generate PMF
protects against salt and temp
protects against Abx
acid-fast
light blue with gram
inner mb, peptidoglycan, mycolic acid (hydrophobic)
What is targeted by Abx
biggest target cell wall (b-lactams, penicillin, vancomycin literally all the host girls)
FtsZ
tubulin like, spherical shape, forms ring participates in septation
MreB
actin like, spiral, elongation, rod