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Bacte (MJ) Flashcards

1
Q

Nutritional requirment for obligate aerobe?

a. O2 = 21% Co2 = 0.03%
b. O2 = 15%, CO2 5-10%
c. O2 = 5% CO2 10%, nitrogen 85%

A

a

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2
Q

Nutritional requirment for capnophile?

a. O2 = 21% Co2 = 0.03%
b. O2 = 15%, CO2 5-10%
c. O2 = 5% CO2 10%, nitrogen 85%

A

b

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3
Q

Nutritional requirment for Microaerophiles?

a. O2 = 21% Co2 = 0.03%
b. O2 = 15%, CO2 5-10%
c. O2 = 5% CO2 10%, nitrogen 85%

A

c

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4
Q

Candle Jar is an aerobic jar

T or F

A

F

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5
Q

In anaerobic incubation, Palladium pellets are used as?

a. Substrate
b. Catalyst
c. Oxidizer
d. Oxygen Reducer

A

b

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6
Q

Indicative of anaerobic system includes methylene and Resazurine, what is the color if the jar contains no oxygen?

a. Red and blue
b. Pink and blue
c. Colorless
d. None of the above

A

c

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7
Q

Organisms that can grow both at 35C and 42 C:

a. Pseudomonas and Campylobacter
b. Pseudomonas and Y. enterocolitica
c. Campylobacter and Y. enterocolitica
d. All of the above

A

a

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8
Q

Mos common organism isolated in blood bags?

A

Yersinia enterocolitica

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9
Q

Specimen consideration:

Blood is collected 30-45 minutes___________

a. After the fever spikes
b. During the fever spikes
c. Before the fever spikes
d. Never collect

A

c

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10
Q

How many sets of blood cultures per 24 hours?

a. 1-2
b. 2-3
c. 3-4
d. 4-5

A

b

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11
Q

Sputum is collected early in the morning for__________

a. 2-3 consecutative days
b. 1-2 Consecutive day
c. 3 Consecutative days
d. 4 consecutive days

A

a

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12
Q

Inoculating loops and needles used for sputum culture should be dibbed in

a. 70% Alcohol
b. Cresol with sand before flaming
c. None
d. A and B

A

d

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13
Q

Digestion reagent for sputum?

a. NALC
b. 2-4% NaOH

A

a

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14
Q

Decontamination reagent for sputum

a. NALC
b. 2-4% NaOH

A

b

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15
Q

Specimen for detection of S. aureus

a. Nasopharyngeal Swab
b. Nasal Swab
c. Throat Swab

A

b

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16
Q

Specimen for detection for B. pertusis and Neisseria menigitidis

a. Nasopharyngeal Swab
b. Nasal Swab
c. Throat Swab

A

a

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17
Q

Specimen for detection of S. pyogenes, Candida albicans and Haemophilus influenzae

a. Nasopharyngeal Swab
b. Nasal Swab
c. Throat Swab

A

c

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18
Q

Significant bacteruria

a. > 10^5 CFU/mL
b. > 10^5 CFU/L
c. <10^5 CFU/mL
d. < 10^5 CFU/L

A

a

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19
Q

Stains:

Capsules of Crytococcus

a. Schaeffer-futon stain
b. Leifson
c. MEthylene blue
d. Wayson
e. India ink

A

e

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20
Q

Stains:

Flagella

a. Schaeffer-futon stain
b. Leifson
c. MEthylene blue
d. Wayson
e. India ink

A

b

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21
Q

Stains:

Metachromatic granules

a. Schaeffer-futon stain
b. Leifson
c. MEthylene blue
d. Wayson
e. India ink

A

c

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22
Q

Stains:

Bipolar bodies

a. Schaeffer-futon stain
b. Leifson
c. MEthylene blue
d. Wayson
e. India ink

A

d

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23
Q

Stains:

YErsinia

a. Schaeffer-futon stain
b. Leifson
c. MEthylene blue
d. Wayson
e. India ink

A

d

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24
Q

Stains:

Endospores

a. Schaeffer-futon stain
b. Leifson
c. MEthylene blue
d. Wayson
e. India ink

A

a

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25
Q

Most critical step in Gram Staining?

A

Decoloriztion

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26
Q

Term for variability in the size and shape of cells

a. Anisomorphism
b. Poikilomorphism
c. Pleochromorphsim
d. Pleomorphism

A

d

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27
Q

Also known as Gram Ghost

A

Mycobacterium

28
Q

Acid fasr organisms in tissues are best stained using?

a. Ziehl neelsen
b. Kinyoun
c. Auramine Rhodamine

29
Q

Secondary stain in Ziehl neelsen include:

a. Methylene blue
b. Malachite green
c. Hematoxylin
d. All of the above

30
Q

Secondary stain in Fite faraco include:

a. Methylene blue
b. Malachite green
c. Hematoxylin
d. All of the above

31
Q

Color of non acid fast?

32
Q

Color of acid fast?

33
Q

Diameter of sputum in the slide during AFB smear preparation?

a. 2-3 cm
b. 2-3 mm
c. 2-3 dm
d. 2-3m

34
Q

Choice of blood in BAP (5% defibrinated blood) according to there preference:

a. Horse
b. Sheep
c. Rabbit

35
Q

Hemolytic pattern where a small zone of alpha surround by a zone of beta hemolysis after refrigeration

a. Double zone of hemolysis
b. Alpha prime
c. Beta prime
d. Gamma prime

36
Q

Staphylococcus sp.

a. Chocolate agar
b. Mannitol salt agar

37
Q

Neisseria, haemophilus

a. Chocolate agar
b. Mannitol salt agar

38
Q

CAP + Vancomycin-Colistin-Nystatin-Trimetoprim

a. Thayer Martin
b. Modified Thayer Martin
c. Martin Lewis Medium
d. New York City Agar

39
Q

CAP + Vancomycin-Colistin-Anisomycin-Trimetoprim

a. Thayer Martin
b. Modified Thayer Martin
c. Martin Lewis Medium
d. New York City Agar

40
Q

CAP + Vancomycin-Colistin-Nystatin

a. Thayer Martin
b. Modified Thayer Martin
c. Martin Lewis Medium
d. New York City Agar

41
Q

CAP + Vancomycin-Colistin-Amphotericin B-Trimetoprim

a. Thayer Martin
b. Modified Thayer Martin
c. Martin Lewis Medium
d. New York City Agar

42
Q

Corynebacterium diphtheriae

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

43
Q

Francisella tularensis

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

44
Q

Campylobacter

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

45
Q

Leigionella pneumophilia

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium TCBS
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth

46
Q

Salmonella

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

47
Q

Bordetella pertusis

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

48
Q

Vibrio

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

49
Q

G. vaginalis

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

50
Q

Mycobacterium

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

51
Q

E. coli O157:H7

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

52
Q

Beta hemolytic Streptococci

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

53
Q

Color of CHO fermenters for XLD?

54
Q

Color of salmonella in XLD?

a. Yellow with black center
b. Red with black center
c. Yellow with Colorless center
d. Red with Colorless center

55
Q

Cefsulodin Irgasan Novobiocin medium :

a. Yersinia pestis
b. Yersinia enterocolitica
c. Yersinia coli
d. Yersinia trachomatis

56
Q

XLD

a. Phenol Red
b. Methyl Red
c. Neutral Red
d. Bromothymol Blue
e. Bromcresol Purple

57
Q

MR test

a. Phenol Red
b. Methyl Red
c. Neutral Red
d. Bromothymol Blue
e. Bromcresol Purple

58
Q

Malonate utilization test

a. Phenol Red
b. Methyl Red
c. Neutral Red
d. Bromothymol Blue
e. Bromcresol Purple

59
Q

Simmon citrate agar

a. Phenol Red
b. Methyl Red
c. Neutral Red
d. Bromothymol Blue
e. Bromcresol Purple

60
Q

Lysine Iron Agar

a. Phenol Red
b. Methyl Red
c. Neutral Red
d. Bromothymol Blue
e. Bromcresol Purple

61
Q

Carbohydrate Fermentation test

a. Phenol Red
b. Methyl Red
c. Neutral Red
d. Bromothymol Blue
e. Bromcresol Purple

62
Q

MAC

a. Phenol Red
b. Methyl Red
c. Neutral Red
d. Bromothymol Blue
e. Bromcresol Purple

63
Q

Urease test, MSA

a. Phenol Red
b. Methyl Red
c. Neutral Red
d. Bromothymol Blue
e. Bromcresol Purple

64
Q

Yersinia is Motile at 22 or 25 C but not at

a. 37C
b. 35C
c. 42 C
d. 31C

65
Q

Substrate for Indole test?

a. KOVACs
b. Indole
c. Tryptophan

66
Q

Test to differentiate klebsiella from enterobacter

a. Ornithine decarboxylase and Motility
b. TSA and LIA
c. Oxidase and Motility
d. Citrate and Ureas

ACTS (Final Coarching (5 decks)

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