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ACTS (Final Coarching > Bacte (MJ) > Flashcards

Bacte (MJ) Flashcards

1
Q

Nutritional requirment for obligate aerobe?

a. O2 = 21% Co2 = 0.03%
b. O2 = 15%, CO2 5-10%
c. O2 = 5% CO2 10%, nitrogen 85%

A

a

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2
Q

Nutritional requirment for capnophile?

a. O2 = 21% Co2 = 0.03%
b. O2 = 15%, CO2 5-10%
c. O2 = 5% CO2 10%, nitrogen 85%

A

b

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3
Q

Nutritional requirment for Microaerophiles?

a. O2 = 21% Co2 = 0.03%
b. O2 = 15%, CO2 5-10%
c. O2 = 5% CO2 10%, nitrogen 85%

A

c

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4
Q

Candle Jar is an aerobic jar

T or F

A

F

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5
Q

In anaerobic incubation, Palladium pellets are used as?

a. Substrate
b. Catalyst
c. Oxidizer
d. Oxygen Reducer

A

b

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6
Q

Indicative of anaerobic system includes methylene and Resazurine, what is the color if the jar contains no oxygen?

a. Red and blue
b. Pink and blue
c. Colorless
d. None of the above

A

c

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7
Q

Organisms that can grow both at 35C and 42 C:

a. Pseudomonas and Campylobacter
b. Pseudomonas and Y. enterocolitica
c. Campylobacter and Y. enterocolitica
d. All of the above

A

a

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8
Q

Mos common organism isolated in blood bags?

A

Yersinia enterocolitica

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9
Q

Specimen consideration:

Blood is collected 30-45 minutes___________

a. After the fever spikes
b. During the fever spikes
c. Before the fever spikes
d. Never collect

A

c

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10
Q

How many sets of blood cultures per 24 hours?

a. 1-2
b. 2-3
c. 3-4
d. 4-5

A

b

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11
Q

Sputum is collected early in the morning for__________

a. 2-3 consecutative days
b. 1-2 Consecutive day
c. 3 Consecutative days
d. 4 consecutive days

A

a

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12
Q

Inoculating loops and needles used for sputum culture should be dibbed in

a. 70% Alcohol
b. Cresol with sand before flaming
c. None
d. A and B

A

d

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13
Q

Digestion reagent for sputum?

a. NALC
b. 2-4% NaOH

A

a

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14
Q

Decontamination reagent for sputum

a. NALC
b. 2-4% NaOH

A

b

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15
Q

Specimen for detection of S. aureus

a. Nasopharyngeal Swab
b. Nasal Swab
c. Throat Swab

A

b

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16
Q

Specimen for detection for B. pertusis and Neisseria menigitidis

a. Nasopharyngeal Swab
b. Nasal Swab
c. Throat Swab

A

a

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17
Q

Specimen for detection of S. pyogenes, Candida albicans and Haemophilus influenzae

a. Nasopharyngeal Swab
b. Nasal Swab
c. Throat Swab

A

c

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18
Q

Significant bacteruria

a. > 10^5 CFU/mL
b. > 10^5 CFU/L
c. <10^5 CFU/mL
d. < 10^5 CFU/L

A

a

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19
Q

Stains:

Capsules of Crytococcus

a. Schaeffer-futon stain
b. Leifson
c. MEthylene blue
d. Wayson
e. India ink

A

e

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20
Q

Stains:

Flagella

a. Schaeffer-futon stain
b. Leifson
c. MEthylene blue
d. Wayson
e. India ink

A

b

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21
Q

Stains:

Metachromatic granules

a. Schaeffer-futon stain
b. Leifson
c. MEthylene blue
d. Wayson
e. India ink

A

c

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22
Q

Stains:

Bipolar bodies

a. Schaeffer-futon stain
b. Leifson
c. MEthylene blue
d. Wayson
e. India ink

A

d

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23
Q

Stains:

YErsinia

a. Schaeffer-futon stain
b. Leifson
c. MEthylene blue
d. Wayson
e. India ink

A

d

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24
Q

Stains:

Endospores

a. Schaeffer-futon stain
b. Leifson
c. MEthylene blue
d. Wayson
e. India ink

A

a

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25
Q

Most critical step in Gram Staining?

A

Decoloriztion

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26
Q

Term for variability in the size and shape of cells

a. Anisomorphism
b. Poikilomorphism
c. Pleochromorphsim
d. Pleomorphism

A

d

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27
Q

Also known as Gram Ghost

A

Mycobacterium

28
Q

Acid fasr organisms in tissues are best stained using?

a. Ziehl neelsen
b. Kinyoun
c. Auramine Rhodamine

A

b

29
Q

Secondary stain in Ziehl neelsen include:

a. Methylene blue
b. Malachite green
c. Hematoxylin
d. All of the above

A

d

30
Q

Secondary stain in Fite faraco include:

a. Methylene blue
b. Malachite green
c. Hematoxylin
d. All of the above

A

c

31
Q

Color of non acid fast?

A

Blue

32
Q

Color of acid fast?

A

Red

33
Q

Diameter of sputum in the slide during AFB smear preparation?

a. 2-3 cm
b. 2-3 mm
c. 2-3 dm
d. 2-3m

A

a

34
Q

Choice of blood in BAP (5% defibrinated blood) according to there preference:

a. Horse
b. Sheep
c. Rabbit

A

b, a, c

35
Q

Hemolytic pattern where a small zone of alpha surround by a zone of beta hemolysis after refrigeration

a. Double zone of hemolysis
b. Alpha prime
c. Beta prime
d. Gamma prime

A

b

36
Q

Staphylococcus sp.

a. Chocolate agar
b. Mannitol salt agar

A

b

37
Q

Neisseria, haemophilus

a. Chocolate agar
b. Mannitol salt agar

A

a

38
Q

CAP + Vancomycin-Colistin-Nystatin-Trimetoprim

a. Thayer Martin
b. Modified Thayer Martin
c. Martin Lewis Medium
d. New York City Agar

A

b

39
Q

CAP + Vancomycin-Colistin-Anisomycin-Trimetoprim

a. Thayer Martin
b. Modified Thayer Martin
c. Martin Lewis Medium
d. New York City Agar

A

c

40
Q

CAP + Vancomycin-Colistin-Nystatin

a. Thayer Martin
b. Modified Thayer Martin
c. Martin Lewis Medium
d. New York City Agar

A

a

41
Q

CAP + Vancomycin-Colistin-Amphotericin B-Trimetoprim

a. Thayer Martin
b. Modified Thayer Martin
c. Martin Lewis Medium
d. New York City Agar

A

d

42
Q

Corynebacterium diphtheriae

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

A

c

43
Q

Francisella tularensis

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

A

d

44
Q

Campylobacter

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

A

f

45
Q

Leigionella pneumophilia

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium TCBS
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth

A

e

46
Q

Salmonella

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

A

a

47
Q

Bordetella pertusis

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

A

b

48
Q

Vibrio

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

A

k

49
Q

G. vaginalis

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

A

h

50
Q

Mycobacterium

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

A

i

51
Q

E. coli O157:H7

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

A

g

52
Q

Beta hemolytic Streptococci

a Bismuth Sulfite agar
b. Regan Lowe agar (Charcoal cephalexin Blood)
c. Cystine-tellurite medium / Modified tinsdale
d. Blood cystine Glucose agar
e. BCYE
f. Skirrows medium
g. Sorbitol MAC
h. Human Blood Tween bilayer medium
i. Middlebrook 7H10 and 7H11
j.Todd-Hewitt broth
k. TCBS

A

j

53
Q

Color of CHO fermenters for XLD?

A

Yellow

54
Q

Color of salmonella in XLD?

a. Yellow with black center
b. Red with black center
c. Yellow with Colorless center
d. Red with Colorless center

A

b

55
Q

Cefsulodin Irgasan Novobiocin medium :

a. Yersinia pestis
b. Yersinia enterocolitica
c. Yersinia coli
d. Yersinia trachomatis

A

b

56
Q

XLD

a. Phenol Red
b. Methyl Red
c. Neutral Red
d. Bromothymol Blue
e. Bromcresol Purple

A

a

57
Q

MR test

a. Phenol Red
b. Methyl Red
c. Neutral Red
d. Bromothymol Blue
e. Bromcresol Purple

A

b

58
Q

Malonate utilization test

a. Phenol Red
b. Methyl Red
c. Neutral Red
d. Bromothymol Blue
e. Bromcresol Purple

A

d

59
Q

Simmon citrate agar

a. Phenol Red
b. Methyl Red
c. Neutral Red
d. Bromothymol Blue
e. Bromcresol Purple

A

d

60
Q

Lysine Iron Agar

a. Phenol Red
b. Methyl Red
c. Neutral Red
d. Bromothymol Blue
e. Bromcresol Purple

A

e

61
Q

Carbohydrate Fermentation test

a. Phenol Red
b. Methyl Red
c. Neutral Red
d. Bromothymol Blue
e. Bromcresol Purple

A

d

62
Q

MAC

a. Phenol Red
b. Methyl Red
c. Neutral Red
d. Bromothymol Blue
e. Bromcresol Purple

A

c

63
Q

Urease test, MSA

a. Phenol Red
b. Methyl Red
c. Neutral Red
d. Bromothymol Blue
e. Bromcresol Purple

A

a

64
Q

Yersinia is Motile at 22 or 25 C but not at

a. 37C
b. 35C
c. 42 C
d. 31C

A

a

65
Q

Substrate for Indole test?

a. KOVACs
b. Indole
c. Tryptophan

A

c

66
Q

Test to differentiate klebsiella from enterobacter

a. Ornithine decarboxylase and Motility
b. TSA and LIA
c. Oxidase and Motility
d. Citrate and Ureas

A

a

67
Q
A

ACTS (Final Coarching (5 decks)

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