Bacte Flashcards

1
Q

Which of the following specimens
would be acceptable for anaerobic
culture?

A. Vaginal and eye discharge
B. Ear and nose discharge
C. Pleural and brain discharge
D. Urine and sputum

A

C. Pleural and brain discharge

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2
Q

Which of the following should best
differentiate Streptococcus agalactiae
from Streptococcus pyogenes?

A. The ability to grow on sodium azide
medium
B. A positive bile esculin test
C. Hydrolysis on sodium hippurate
D. Beta hemolysis on sheep blood agar

A

C. Hydrolysis on sodium hippurate

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3
Q

Components of gram stain?

A

Crystal violet,
grams iodine,
acetone
alcohol,
safranin

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4
Q

SPS is used as an anticoagulant for
blood culture because it.

A

Inactivates neutrophils and
components of serum complement

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5
Q

Thioglycolate broth is stored at room
temperature and in dark so that

A

d.There is a decreased absorption of
oxygen in the medium

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6
Q

Mac Conkey is considered what type
of media?

A

Selective and Differential

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7
Q

Which of the following organisms
must be incubated in a microaerophilic
environment for optimal recovery of the
organisms?

A

C. jejuni

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8
Q

When performing a Kovac’s indole
test, the substrate must contain?

A

Tryptophan

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9
Q

Thioglycolate broth is stored at room
temperature and in dark so that

A

There is a decreased absorption of
oxygen in the medium

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10
Q

When processing throat swab for a
streptococcus culture, the medium of
choice is ?

A

Sheep blood agar plate

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11
Q

Shigella species characteristically
are?

A

non-motile

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12
Q

An antibiotic that inhibit cell wall?

A

Penicillin

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13
Q

A gram positive coccus isolated
from blood culture has the following
characteristics. Optochin (-); Hippurate
hydrolysis (+); Bacitracin (-) ; Catalase
(-) Bile esculin hydrolysis (-) This
organism is most likely:

A

S. agalactiae

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14
Q

H. parainfluenza requires growth
factor for growth. This are?

A

X and V

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15
Q

Presumptive test for Streptococcus
pneumoniae?

A

Optochin

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16
Q

N. gonorrhea ferments?

A

Glucose only

17
Q

H. influenza requires growth factors
which are?

A

NAD and Hemin

18
Q

Test used to diffentiate
Staphylococcus from Streptococcus?

A

Catalase

19
Q

All mycobacteria stain are?

A

Acid fast

20
Q

E. coli is always citrate?

A

Neg

21
Q

Proteus mirabilis is ornithine?

A

Pos

22
Q

Gonorrhea must be grown on

A

Thayer Martin at 37 degrees with
5-10% Carbon dioxide

23
Q

Carbohydrates found on TSI?

A

glucose, lactose, sucrose

24
Q

Staphylococcus saprophyticus is?

A

Ornithine decarboxylase resistant

25
Q

Staphylococcus epidermidis is?

A

Coagulase (-)

26
Q

X factor is also known as?

A

Hemin

27
Q

Former name of Pseudomonas
maltophilia?

A

Xanthomonas maltophilia

28
Q

TCBS means?

A

Thioglycolate Citrate Bile Salt
Sucrose Agar