Background 3 anaerobes

Question 1

what is a means of identifying anaerbes in agar?

Answer 1

add metronidazole impregnanted discs to the surface

Question 2

what does metronidazole do to agar

Answer 2

it inhbiits the growth of strict anaeobes and is seen as a zone of organism inhibition around the disc

Question 3

there are two major subgroups of anaerobes, these are

Answer 3

non spore forming anaerobes and spore forming anaerobes

Question 4

non spore forming anaerobes die quickly when

Answer 4

exposed to air

Question 5

what is an example of non spore forming anaerobes

Answer 5

bacterioides group

Question 6

spore forming anaerobes form

Answer 6

spores which are resistant to drying, heat and disinfectants

Question 7

an example of spore forming anaerobes is

Answer 7

clostridia group

Question 8

are clostridia gram pos or gram neg rods

Answer 8

gram pos rods

Question 9

clostridium botulinum describe

Answer 9

gram pos rods with oval shaped sub terminal spores

Question 10

c botulinum produces a toxin when contaminated food samples are

Answer 10

heated/cooked

Question 11

the toxin produced by c.botulinum causes food

Answer 11

poisoning which can cause paralysis and death

Question 12

describe clostridium perfringens

Answer 12

beta haemolutic gram pos rod

Question 13

how is clostridium perfringens identified

Answer 13

using a nagler plate

Question 14

what does a nagler plate contain

Answer 14

egg yolk

Question 15

what does a nagler plate show the presence of

Answer 15

toxin lecithinase

Question 16

what can c.perfringens cause?

Answer 16

gas gangrene and food posioning

Question 17

clostridium tetani are non haemolytic gram

Answer 17

positive rods with round terminal spores

Question 18

what does clostridium tetanni release

Answer 18

a toxin affecting the nervous system - tetanus

Question 19

clostridium difficile is found in the gut of

Answer 19

many healthy humans and can cause diarrhoea when normal balance of flora disrupted