Baby - CM Flashcards
Moderate Bacteria
10-50 HPF
Many mucus seen
> 10 LPF
RBCs, WBCs
Average number per 10 HPF
Casts
Average number per LPF
Squamous epithelial cells
Rare, few, moderate or many per LPF
Transitional epithelial cells
Rare, few moderate or many per HPF
RTE cells
Average number per 10 HPF
Oval fat bodies
Average number per HPF
Bacteria, yeast
Rare, few, moderate or many per HPF, presence of WBC’s may be required
Trichomonas
Rare, few, moderate or many per HPF
Spermatozoa
Present, based on laboratory protocol
Mucus
Rare, few moderate or many per LPF
Normal crystals
Rare, few moderate or many per HPF
Abnormal cyrstals
Average and reported per LPF
Serious hazard degree
3
Turn around time (TAT)
Time from ordering a test through analysis in the lab to the charting of the report
Chain of custody (chain of evidence)
Step by step documentation of handling and testing of legal specimens. It begins with patient identification and continues until testing is completed and results reported
Drug testing, COC
30 to 45 mL urine (60 mL container capacity)
32.5 to 37.7°C temperature
Routine urinalysis
10 to 15 mL urine, ave. 12 mL
50 mL container capacity
Glucose reabsorption
Proximal convoluted tubules
Normally, 60% to 75% of filtered Na+
Reabsorbed in PCT
Active transport of sodium
PCT and DCT
Passive transport of sodium
Ascending loop of Henle
Impermeable to water
Ascending loop of Henle
Highly permeable to water but poorly permeable to solutes
Descending loop of Henle
Urine color
Good light source, looking down through the container against a white background
Urine clarity
Visually examining the mixed specimen while holding it in front of a light source, clear container
Carotene
Yellow urine, orange CSF
Red-brown urine, children
Fuchsin (candy, food)
Brown or black urine on standing but negative chemical test for blood
Melanin (melanoma) Homogentisic acid (alkaptonuria)
Print blurred through urine
Cloudy
Calibration of refractometer, 5% NaCl
1.022 ± 0.001
Calibration of refractometer, 9% sucrose
1.034 ± 0.001
Cabbage odor urine
Methionine malabsorption
Bence Jones protein coagulates at
40-60°C
Bence Jones protein dissolves at
100°C
Significant value of AER (Albumin excretion rate)
20-200 ug/min
Principle of Micral test
EIA
11th pad in the reagent strip
Vitamin C, ascorbic acid
Differentiates hemoglobin from myoglobin
Blondheim’s test
Ammonium sulfate ppt. hemoglobin
Nomarski microscope
Interference contrast microscope
RBC’s in hypotonic urine
Ghost cells
WBC’s in hypotonic urine
Glitter cells
Lipid-containing RTE cells
Oval fat bodies, lipidura - nephrotic syndrome
RTE cells with non-lipid-containing vacuoles
Bubble cells, acute tubular necrosis
Casts
Fromed in DCT and CT
Lemon-shaped crystal
Uric acid
Ethylene glycol poisoning
Monohydrate calcium oxalate, oval or dubbell
Ampicillin crystals
colorless needles that tend to form bundles following refrigeration
Sulfonamide crystals
Yellow-brown, occasionally colorless sheaves of wheat, rosettes, needles, confirmed by diazo reaction
Flat, rectangular plates or prisms
Calcium phosphate, apatite
Apatite
Calcium phosphate
Weddelite
Dihydrate calcium oxalate, envelope or pyramidal
Whewellite
Monohydrate calcium oxalate, oval, dumbbell
Struvite
Triple phosphate magnesium ammonium phosphate
Renal calculi may form in the
Calyces and pelvis of the kidney, ureters, and bladder
Cacluli: yellow to brownish red, mod. hard
Uric acid and urate stones
Calculi: pale and friable
Phosphate stones
Calculi: very hard, dark color, rough surface
Calcium oxalate stones
Calculi: yellow-brown resembling an old soap, somewhat greasy
Cystine stones
CTAB, cetyltrimethyl ammonium bromide
Mucopolysaccharides
(+) White turbidity
Crystal seen in Fanconi’s syndrome
Cystine
Dilution of bloody CSF
1:200 (may be 1:20 in board exams)
Red, brown seminal fluid
blood
Critical to ovum penetration
Acrosomal cap
Size of the acrosomal cap
1/2 of the head and covers 2/3 of the nucleus
Most common cause of male infertility
Varicocele, hardening of the veins that drain the testes
Undiluted seminal fluid, sperms immobilized by heat
Makler counting chamber
Traditional diluting fluid for seminal fluid
Formalin, sodium bicarbonate
Other diluting fluid for seminal fluid
Distilled water, saline
Kruger’s strict criteria
Measurement of the size of the head, neck and tail of the sperm using a micrometer or morphometer, not a routine but recommended by WHO
Test for seminal fluid fructose
Analyzed within 2 hours or frozen
Florence test, choline
Iodine, potassium iodide
(+) Dark brown rhombic crystals
Barbiero’s test, spermine
Picric acid, tricholoroacetic acid
(+) Yellow leaf-shaped crystals
Synovial fluid
Clear and pale yellow, does not clot
Normal volume <3.5 mL
Diluents for synovial fluid
Normal saline (with methylene blue to stain WBC nuclei) To lyse the RBC's, hypotonic saline (0.3% NaCl) or saline with saponin
Normal synovial fluid glucose
Should not be more than 10 mg/dL lower than the blood value
Gout, monosodium urate, uric acid
Yellow (-) birefringence under a compensated polarizing light
Pseudogout, Calcium pyrophosphate
Blue (+) birefringence under a compensated polarizing light
Calcified cartilage degeneration, osteoarthritis, Calcium phosphate
No birefringence
Normal appearance of pleural, peritoneal, and pericardial fluid
Clear and pale yellow
Tuberculous pleuritis
Adenosine deaminase 40 U/L or greater
Mesothelial cells are pleomorphic
Res. lymphocytes, plasma cells, and malignant cells
Reactive mesothelial cells (pleural)
Closely resembling malignant cells
Increased mesothelial cells
Pneumonia malignancy
Lack of mesothelial cells
Tuberculosis
Psammoma bodies
Concentric striations of collagen-like materials
Benign conditions, ovarian and thyroid carcinoma
Peritoneal fluid, bacterial peritonitis, cirrhosis
> 500 WBCs/uL
Peritoneal lavage, blunt trauma
> 100,000 RBCs/uL
Peritoneal fluid, amylase
Increased in pancreatitis and G.I. perforation
Peritoneal fluid, alkaline phosphatase
Increased in G.I. perforation
Dark green amniotic fluid
Meconium
Dark red brown amniotic fluid
Fetal death
Secreted by the type II pneumocytes of the fetal lungs
Lamellar bodies which are densely packed layers of phospholipids that represent a storage form of pulmonary surfactant. Increases O.D. of amniotic fluid (O.D. 650)
Inserted through the nose (Leah Navarro)
Levin Tube
Inserted through the mouth
Rehfuss tube
Tubeless test for gastric acidity
Diagnex tubeless test, urine sp., Azure blue; breath test
Positive result with guaiac
Blue
Creola bodies
Cluster of columnar cells, bronchial asthma
Normal stool pH
pH 7-8
Carbohydrate disorders, stool pH
pH below 5.5
Number of fecal leukocytes indicative of an invasive condition
≥ 3 neutrophils/hpf
Blue quadrant
Health
Red quadrant
Fire
White quadrant
Specific hazard
Yellow quadrant
Reactivity
RACE
Rescue
Alarm
Contain
Extinguish/Evacuate
PASS
Pull
Aim
Squeeze handles (trigger)
Sweep
PDCA
Plan-Do-Check-Act
PDSA
Plan-Do-Study-Act
Calibration of centrifuge
Every 3 month
Disinfection of centrifuge
weekly basis
IRIS
International Remote Imaging System; 2 mL