Baby - CC Flashcards

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1
Q

Bilirubin conversion factor mg/dL to umol/L

A

17.1

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2
Q

Creatinine conversion factor mg/dL to umol/L

A

88.4

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3
Q

Sodium, Potassium, Chloride conversion factor mEq/L to mmol/L

A

1

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4
Q

TP/Albumin/globulin g/dL to mg/L

A

10

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5
Q

Ig conversion factor from mg/dL to mg/L

A

10

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6
Q

Ig conversion factor from mg/dL to g/L

A

0.1

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7
Q

Thryoxine ug/dL to nmol/L

A

12.9

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8
Q

BUN conversion factor from mg/dL to mmol/L

A

0.357

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9
Q

BUN to Urea

A

2.14

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10
Q

Urea to BUN

A

0.467

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11
Q

Normal BUN: Creatinine ratio

A

10-20:1

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12
Q

Creatinine clearance

A

Indirect method used to asses the glomerular filtration functioning capabilities of the kidneys

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13
Q

Creatinine

A

Index of overall renal function

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14
Q

B2 microglobulin

A

Appears in the urine when reabsorption is incomplete because of proximal tubular damage, as in acute kidney injury

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15
Q

Assay for urea that is inexpensive but lacks specificity

A

Colorimetric, diacetyl

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16
Q

Assay for urea that measures ammonia formation

A

Enzymatic

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17
Q

Simple nonspecific method for creatinine

A

Colorimetric, endpoint

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18
Q

Assay for creatinine which is rapid and with increased specificity

A

Colorimetric: kinetic

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19
Q

Assay for creatinine that measures ammonia colorimetrically or with ion-selective

A

Enzymatic

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20
Q

Assay for uric acid, problems with turbidity

A

Colorimetric

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21
Q

Asssay for uric that needs special instrumentation and optical cells

A

Enzymatic: UV

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22
Q

Assay for uric acid, interference by reducing substances

A

Enzymatic: H2O2

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23
Q

A progressive and irreversible loss of renal function, results from several disease entities

A

Chronic renal failure; Progressive and irreversible

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24
Q

Least interference with analysis; Natural

A

Heparin

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25
Q

Heparin for most chemistry tests; common

A

Lithium heparin

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26
Q

Glucose is metabolized at a room temperature

A

7 mg/dL/hour

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27
Q

Glucose is metabolized at 4degC

A

2 mg/dL/hour

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28
Q

Hypoglycemia

A

Blood glucose level less then 50 mg/dL

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29
Q

OGTT

A

Patient should be ambulatory; fasting of 8 to 14 hours

Unrestricted diet of 150 grams CHO/day for 3 days prior to testing

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30
Q

Performed routinely to monitor glucose control

A

Glycosylated hemoglobin

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31
Q

Gestational diabetes patients develop diabetes

A

Within 5 to 10 years

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32
Q

Sodium concentration in patient with DM

A

Decreased due to polyuria

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33
Q

Cholesterol [NCEP Guideline for acceptable measurement error]

A

CV ≤ 3%

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34
Q

Triglycerides [NCEP Guidelines for acceptable measurement error]

A

CV ≤ 5%

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35
Q

LDLs, HDLc [NCEP Guidelines for acceptable measurement error]

A

CV ≤4%

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36
Q

Minor lipoproteins

A

IDL and Lp (a)

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37
Q

Major structural protein in HDL

A

Apo A1

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38
Q

Major structural protein in VLDL and LDL

A

Apo B100

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39
Q

Structural protein in chylomicrons

A

Apo B48

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40
Q

LDL cholesterol may be calculated from measurements of:

A

TC, TAG and HDL-c

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41
Q

Floating beta-lipoprotein

A

B-VLDL

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42
Q

Sinking pre-beta lipoprotein

A

Lp (a)

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43
Q

HDL cholesterol protective against heart disease

A

≥ 60mg/dL

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44
Q

HDL cholesterol major risk for heart disease

A

<40 mg/dL

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45
Q

Serum cholesterol: moderate risk>170 mg/dL, high risk >185 mg/dL

A

2-19 years old

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46
Q

Serum cholesterol: moderate risk>200 mg/dL, high risk >220 mg/dL

A

20-29

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47
Q

Serum cholesterol: moderate risk>220 mg/dL, high risk >240 mg/dL

A

30-39 years old

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48
Q

Serum cholesterol: moderate risk>240 mg/dL, high risk >260 mg/dL

A

40 and over

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49
Q

One-step, direct method for cholesterol

A

Libermann-Burchardt (L-B) procedure

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50
Q

Current reference method for cholesterol

A

Abell-Kendall method

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51
Q

PiSO

A

Potassium: Inside
Sodium: outside

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52
Q

Chloride

A

Counterion of sodium

Counterbalance of sodium

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53
Q

Routinely measured electrolytes

A

Sodium, potassium, chloride and bicarbonate

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54
Q

Largest contribution to the osmolality value of serum

A

Sodium, chloride and bicarbonate

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55
Q

Osmolality of plasma

A

2 Na + Glucose (mg/dL) / 20 + BUN (mg/dL) / 3

Or

1.86 Na + glucose/18 + BUN/2.8 + 9

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56
Q

Osmolal gap

Difference between the measured osmolality and calculated osmolality

A

Indirectly indicates the presence of osmotically active substances other than Na+, urea, or glucose, such as ethanol, methanol, ethylene glycol, lactate, or B-hydroxybutyrate

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57
Q

Anion

Na - (Cl + HCO3)

A

NV: 7-16 mmol/L

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58
Q

Anion gap

Na + K) - (Cl + HCO3

A

NV: 10-20 mmol/L

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59
Q

Anion gap exceeds 16 mmol/L

A

Indication of increased concentrations of the unmeasured anions (PO4 3-, SO4 2-, proteins ions

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60
Q

ADIC

A

Anion Decrease
Increase Cation

Decrease anion gaps of less than 10 mmol/L

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61
Q

The anion gap is also useful as a QUALITY CONTROL measure for electrolyte results

A

If an increased anion gap is gap is found for electrolytes in a healthy person, one or more of the test results may be erroneous, and the tests should be repeated

62
Q

Most common cause of hyperkalemia in hospitalized patients

A

Due to therapeutic K+ administration.

The risk is greatest with IV K+ replacement.

63
Q

Hypernatremia

A

Increased intake or retention
Conn’s disease
Hyperaldosteronism

64
Q

Most abundant cation in the ECF

A

Sodium

65
Q

Hyponatremia

A

Increased water retention

Renal failure

66
Q

Major intracellular cation in the body, Integral part of the transmission of nerve impulses

A

Potassium

67
Q

Nonspecific but sensitive for renal disease

A

Urea

68
Q

BUN to Crea in renal disease

A

Maintained or NORMAL

69
Q

Test for overall renal function; Tubular injury:

A

Crea, B2-Microglobulin

70
Q

Test that requires mercury lamp:

A

Enzymatic UV

71
Q

Short term glucose testing:

A

Fructosamine “glycosylated albumin” (2-3 wks)

72
Q

Not needed for the measurement of LDLc

A

VLDLc - computed value

73
Q

Hormones that regulate calcium

A

PTH, Calcitonin, Vit. D

74
Q

Hyperkalemia

A

Decreased renal excretion
Acute or chronic renal failure

Increased intake

75
Q

Hypokalemia

A

Gastrointestinal loss

76
Q

Major extracellular anion

A

Chloride

77
Q

Inversely related with Chloride

A

Bicarbonate

78
Q

Calcium distribution in blood

A

50% ionized

79
Q

Respiratory acidosis

A

Increase pCO2

80
Q

Respiratory alkalosis

A

Excess CO2 loss

81
Q

Metabolic acidosis

A

Excess H+ production

82
Q

Metabolic alkalosis

A

Excess H+ loss

83
Q

Fever will decrease pO2 by:

A

7%

84
Q

Fever will increase pCO2 by:

A

3%

85
Q

Potentiometry

A

pH, pCO2

86
Q

Amperometry

A

pO2, Polarography

87
Q

89 to 90% of all the CO2 in serum

A

Form of Bicarbonate (HCO3-)

88
Q

Driving force of the Bicarbonate buffer system

A

Carbon dioxide

89
Q

Lock-and-Key (EMIL FISCHER)

A

The shape of the key (substrate) must fit into the lock (enzyme)

90
Q

Induced-Fit (Daniel Koshland)

A

Substrate binding to the active site of the enzyme

91
Q

Fixed-time

A

Reactants are combined; reaction proceeds for a designated time; reaction is stopped and measurement is made

92
Q

Continuous monitoring/kinetic

A

Multiple measurements of absorbance are made during the reaction; more advantageous

93
Q

Forward reaction for CK

A

Tanzer-Gilvarg

94
Q

Reverse reaction for CK

A

Oliver-Rosalki

95
Q

Forward reaction for LD

A

Wacker

96
Q

Reverse reaction for LD

A

Wroblewski LaDue

97
Q

High specificity for erythrocyte, prostate

A

Acid phosphatase

98
Q

High specificity for the liver

A

Alanine aminotransferase

99
Q

High specificity for the pancreas

A

Lipase

100
Q

High specific for the pancreas, salivary gland

A

Amylase

101
Q

Moderate specificity for liver, heart, skeletal muscles

A

Aspartate aminostranferase

102
Q

Moderate specificity for heart, skeletal muscles, brain

A

Creatinine kinase

103
Q

Low specificity for liver, bone and kidney

A

Alkaline phosphatase

104
Q

Most potent of the estrogens

A

Estradiol

105
Q

Hypersecretion of growth hormone in adult

A

Acromegaly

106
Q

Confirmatory test for acromegaly

A

Glucose suppression test - OGTT

107
Q

Effect of growth hormone to blood glucose

A

Increased

108
Q

Cushing’s disease description

A

Increase in cortisol caused by excessive development and activity of pituitary gland

109
Q

Cretinism

A

Hyposecretion of thyroxine in children

110
Q

T3 and T3 uptake test in hypothyroidism

A

Both decreased

111
Q

Computed from Henderson-Hasselbach equation

A

HCO3- (Bicarbonate)

112
Q

T3 and T3 uptake test in hyperthydoidism

A

Both increased

113
Q

Relationship of T3 uptake and T3 test

A

Direct

114
Q

Relationship of T3 uptake test and TBG

A

Inverse

115
Q

Cholesterol and triglycerides in hypothyroidism

A

Increased

116
Q

ACTH is from

A

Anterior pituitary gland

117
Q

Conn’s Syndrome

A

Aldosterone-secreting adrenal adenoma

118
Q

Constituents of a number of common foods, including bananas, vanilla, tea and coffee

A

May react in the test for HMMA (VMA)

119
Q

Enzyme produced by the kidney which acts on angiotensin from angiotensin II

A

Renin

120
Q

Corrects renal blood flow by causing vasodilation of the afferent arterioles and constriction of the efferent arterioles, stimulating reabsorption of sodium and water in the proximal convoluted tubules, and triggering the release of the sodium-retaining hormone aldosterone by the adrenal cortex and antidiuretic hormone by the hypothalamus

A

Angiotensin II

121
Q

Increased antidiuretic hormone

A

Fluid retention, low serum Na+

122
Q

Decreased antidiuretic hormone

A

Fluid loss

123
Q

Increased aldosterone

A

Hypertension, low serum K+

124
Q

Decreased aldosterone

A

Low serum Na+, high serum K+

125
Q

Increased renin

A

Hypertension

126
Q

“Confused teenager; 27 Pregnant; 50 dead”

A
  1. 18%-0.30% - Mental confusion, impaired motor skills
  2. 27%-0.40% - unable to walk, vomiting, impaired consciousness
  3. 35%-0.50% - coma and possible death
127
Q

Presumptive evidence of driving under the influence of alcohol

A

0.10%

>100 mg/dL

128
Q

Metabolite of cocaine

A

Bezoylecgonine

129
Q

BMI of obese

A

≥30kg/m²

130
Q

Blood pressure cuff as tourniquet

A

60 mmHg

40 mmHg during WBLD collection

131
Q

Air displacement pipet

A

Relies on piston for suction to draw sample into disposable tip; the piston does not come in contact with the liquid

132
Q

Positive displacement pipet

A

Operates by moving the piston in the pipet tip or barrel, much like a hypodermic syringe; it does not require a different tip for each use

133
Q

Horizontal centrifuge/swinging bucket

A

Horizontal position in the centrifuge when spinning and a vertical position when the head is not moving

134
Q

Most basic pipet

A

Glass pipet

135
Q

“DpOMA”

A

Drop in pO2 = metabolic acidosis

136
Q

Cushing’s disease

A

Hypersecretion of ACTH

137
Q

Cushing’s syndrome

A

Increased cortisol

138
Q

Fastest centrifuge

A

Ultra centrifuge

139
Q

Monolayer centrifuge

A

Cyto centrifuge

140
Q

“SPiCY”

A

Slope = Prop. Sys. Error

Y intercept = Constant sys. Error

141
Q

“HAXI”

A

X-Axis Horizontal, Abscissa, Independent Variables

142
Q

“VDOY”

A

Y-Axis, Vertical, Ordinate, Dependent Variables

143
Q

Reliability

A

Ability of an analytical method to maintain accuracy and precision over an extended period of time during which equipment, reagent, and personel may change

144
Q

“SPF”

A

F-Test = Precision, S.D.

145
Q

“TAM”

A

T-Test = Accuracy, mean

146
Q

Control that continue to either increase or decrease over a period of 6 consecutive days

A

Trend

147
Q

Six or more consecutive daily values that distribute themselves on one side or either side of mean

A

Shift

148
Q

The smaller the CV

A

Greater is the precision

149
Q

Point-of-care testing (POCT)

A

Alternate site testing, near-patient testing, decentralized testing, bedside testing, or ancillary testing

150
Q

Fahey RID

A

d = log Ag. Conc.

Measurement of the diameters of all circles at a set time after initiation of the diffusion process, measurements are made 24 hours (or 18 hours) after addition of samples to the plate, preferable since results are available much sooner

151
Q

Mancini RID technique

A

d² = Ag. Conc.

Measurements of the diameters after diffusion has ceased, often requires 2 to 3 days before results are available; provide a more reliable estimation of low levels of antigen.

Increased sensitivity