B8 - Gas exchange and Respiration Flashcards
Respiration
An enzyme controlled chemical process that involves the breakdown of nutrient molecules (specifically glucose) in order to release the energy stored within the bonds of these molecules
* it occurs in all living cells
Aerobic respiration
The chemical reactions in cells that use oxygen to break down nutrient molecules to release a large amount of energy
* most of the chemical reactions in aerobic respiration take place in the mitochondria
Anaerobic respiration
The chemical reactions in cells that break down nutrient molecules to release energy without using oxygen and release a relatively small amount of energy
* produces different breakdown products depending on the type of organism it is taking place in
What processes are carried out as a result of respiration? (name 5)
- Muscle contraction
- Protein synthesis
- Cell division (to make new cells)
- Growth
- Active transport across cell membranes
- Generation of nerve impulses
- Maintaining a constant internal body temperature
Measuring the rate of respiration in yeast cells using methylene blue
- Add the dye to a suspension of yeast cells (doesn’t damage cells)
- The time taken for the methylene blue to discolour (lose its colour) is a measure of the rate of respiration of the yeast cells in the suspension - the faster the change the faster the respiration
Why is methylene blue a suitable indicator for measuring the rate of respiration in yeast?
If methylene blue stain is applied to a sample, a healthy cell with turn the stain colorless.
* This is due to the cell’s enzymes, which reduce the methylene blue, causing it to lose its color.
Apparatus needed for investigating the rate of respiration in yeast with a change in temperature
- Yeast suspension
- Glucose solution
- Test tubes
- Stopwatch
- Methylene blue
- Temperature-controlled water bath(s)
Independent variable
the variable that is changed on purpose
Dependent variable
the variable that is measured, i.e. the variable that depends on the independent variable for its outcome
Control variables
The variables that are kept the same in an experiment to ensure accurate results
What are the control variables when investigating the effects of temperature on the rate of respiration in yeast cells?
- Volume/concentration of methylene blue added
- Volume/concentration of yeast suspension
- Concentration of glucose
- pH
What are the results of the experiment on the yeast?
- As the temperature increases up to 40 °C, the rate of respiration increases so the time taken for the solution to become colourless reduces
* this is because the enzymes will increase to optimum temperature where they work best * As temperature increases above 40 °C, the rate of respiration decreases so the time taken for the solution to become colourless increases
* this is because the enzymes will start to denature
Suspension meaning
The state in which the particles of a substance are dispersed but not totally dissolved in a fluid
Word equation for aerobic respiration
glucose + oxygen -› carbon dioxide + water
Balanced chemical equation for aerobic respiration
C(6)H(12)O(6) + 6O(2) -› 6CO(2) + 6H(2)O
Explain anaerobic respiration in animals
Takes place in muscle cells during vigorous exercise
* muscles have higher demand for oxygen than when resting and body can only produce so much using aerobic respiration
* as much glucose as possible is broken down with oxygen, and some glucose is broken down without it, producing lactic acid instead
* there is still energy stored within the bonds of lactic acid molecules that the cell could use; for this reason, less energy is released when glucose is broken down anaerobically
Explain the uses of anaerobic respiration in yeast
- Used during bread making where carbon dioxide is produced causing the bread to rise
- Used in brewing (production of beer) where ethanol and carbon dioxide is produced
Balanced equation for anaerobic respiration in yeast
C(6)H(12)O(6) -› 2C(2)H(5)OH + 2CO(2)
Explain the process of repaying oxygen debt
- Lactic acid builds up in muscle cells and lowers the pH of the cells (making them more acidic)
- This could denature the enzymes in cells so it needs to be removed
- Cells excrete lactic acid into the blood
- When blood passes through the liver, lactic acid is taken up into liver cells where it is oxidised, producing carbon dioxide and water which are waste products (aerobic respiration of lactic acid)
- This is the reason we continue to breath heavily and our heart rate remains high even after finishing exercise
- we need to transport the lactic acid from our muscles to the liver, and continue getting larger amounts of oxygen into the blood to oxidise the lactic acid
Features of gas exchange surfaces (4)
- Large surface area: to allow faster diffusion of gases
- Thin walls: for short distance
- Good ventillation with air: so that the diffusion gradients can be maintained
- Good blood supply: maintain high concentration gradent for faster diffusion