B3.003 Prework 2 Intro to Lab Diagnostics No Culture Flashcards
What are advantages of molecular microbiology
increased sensitivity over culture
rapid
What are limitations of molecular microbiology
Expensive
Targeted
No susceptibility testing
No test of cure
What methods are there for molecular microbiology testing
Nucleic acid hybridization
NAAT
Sequencing
Mass Spec
Describe the steps of PCR
Extract NA from sample
Head DNA to denature
Cool to anneal primers
Elongation
Repeat leading to exponential synthesis of DNA
Fluorescent reporter allows fro detection and quantification
What is NAAT
Amplification and detection
What types of NAAT are there
Single target
Multiple targets
Rank types of sequencing in order of most sensitive to least sensitive
Single > Multiplex»_space; Broad range
Describe the technique of MALDI-TOF MS
Isolate spotted onto metal plate Laser vaporized bacterial proteins and matrix Protiens travel up vacuum tube Detector/analyzer creates profile Profile compared to database
What types of serology are there
Antigen and Antibody
What is the method of Ag serology
use lab generated antibodies to detect patient antigen specimen
What is the method of Ab serology
use lab generated antigens to detect IgG, IgM or total antigen specific patient antiboides
Describe IgM
Associated with acute infection
Detectable within 7 days of infection
Wanes after 2-3 months
Describe IgG
Associated with acute or past infection
Detectable within 14 days
Detectable for entire life
What is agglutination
Antibody or antigen fixed to latex beads
Specimen added to lated Ab or latex Ag leads to visible clumping of beads
Whats an example of a later flow immunoassay
pregnancy test
