B3 practical investigate the effect of pH on the rate of reaction of amylase enzyme

Question 1

explain the steps of this reaction

Answer 1

1. put a drop of iodine solution into every well of a spotting tile
2. place a bunsen burner on a heat proof may and a tripod and gauze over the bunsen burner and heat the water until its 35 degrees C
3. use a syringe to add 1cmcubed of amylase solution and 1cmcubed of a buffer solution pH5 to the boiling tube and put the tube into the beaker of water, wait 5 mins
4. add 5cmcubed of a starch solution to the boiling tube
   5.mix and start a stop clock
5. record how long it take the amyalse to break down all of the starch. drop iodnie solution in every 30 secs- when the iodine solution remains browny-orange starch is no longer present
6. repeat with buffer solutions of different pH values to see how pH affects the time taken for the starch to be broken down.

Question 2

give a control variable of this experiment

Answer 2

concentration and volume of amylase solution