B3 Organisation And Enzymes Flashcards

1
Q

What is an enzyme?

A

A biological catalyst

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2
Q

What is a catalyst?

A

A substance which increases the speed of a reaction, without being changed or used up in the reaction

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3
Q

What is the active site?

A

A unique shape that fits onto the substances involved in a reaction

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4
Q

What happens if the substrate does not fit into the active site?

A

The reaction will not be catalysed

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5
Q

What does changing the temperature of an enzyme catalysed reaction do?

A

Change the rate

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6
Q

What initially happens in an enzyme catalysed reaction with an increase in temperature?

A

The rate increases

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7
Q

What happens to an enzyme when the temperature gets too high?

A

The bonds of the enzymes break
This means the active site changes shape so the substrate does not fit
This is when the enzyme is denatured

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8
Q

What is meant by the optimum temperature of an enzyme?

A

Where the enzyme is most active

this means there are the maximum number of successful collisions between the active site and substrate

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9
Q

How does the pH affect enzymes?

A

If the pH is too high or too low, the bonds are interfered with. This changes the shape of the active site so denatured the enzyme

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10
Q

What is meant by the optimum pH?

A

Where the enzyme is most active.

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11
Q

What do you digestive enzymes break down?

A

A breakdown big molecules into smaller ones

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12
Q

Why do digestive enzymes break down molecules?

A

The broken down molecules smaller and more soluble so they can pass through the digestive system
This is an absorbed into the bloodstream

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13
Q

What do carbohydrases breakdown?

A

Carbohydrates into simple sugars

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14
Q

Where is amylase made?

A

Salivary glands
Pancreas
Small intestine

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15
Q

What do proteases breakdown?

A

Proteins into amino acids

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16
Q

Where are proteases made?

A

Stomach – Pepsin
Pancreas
Small intestine

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17
Q

What does lipases convert?

A

Lipids into glycerol and fatty acids

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18
Q

What are lipids?

A

Fats and oils

19
Q

Where are lipases made?

A

Pancreas

Small intestine

20
Q

What are the products of digestion used for?

A

Used to make new carbohydrates, proteins, lipid

Glucose is used for respiration

21
Q

Where is bile produced and stored?

A

It is produced in the liver

Stored in the gallbladder

22
Q

What does bile do?

A

It’s neutralises the acid from the stomach to make the enzymes in the small intestine work at optimum pH
It’s emulsifies fats to give them a bigger surface area so the enzymes digest them faster

23
Q

Why is hydrochloric acid produced in the stomach?

A

To kill bacteria

To give the right pH for protease enzymes

24
Q

What does Benedict’s reagent test for?

A

Reducing sugars

25
Q

What colour does Benedict’s reagent change in the presence of reducing sugars?

A

From blue to green, yellow or brick red

26
Q

What does iodine solution test for?

A

Starches

27
Q

What colour does iodine solution change to?

A

From browny orange to black or blue black

28
Q

What does biuret’s solution test for?

A

Proteins

29
Q

What colour does biuret’s solution change to?

A

From blue to purple

30
Q

What does Sudan III test for?

A

It stains lipids

31
Q

What colour does Sudan III solution change to?

A

Two separate layers with the top layer being bright red

32
Q

What are the hazards of food test?

A

Iodine is an irritant to the eyes

Biuret’s solution is dangerous Sudan three stain solution is flammable

33
Q

What safety precautions should you take during food tests?

A

Wear goggles
Wash chemicals of skin
Keep Sudan III stain solution away from Bunsen burners

34
Q

what is a tissue?

A

a group of cells with a similar structure and function

35
Q

what is an organ?

A

a group of tissues working together for a specific function

36
Q

what is an organ system?

A

a group of organs

37
Q

what does the churning action in the stomach do?

A

turns food into a liquid so it has a larger surface area to digest

38
Q

what is a substrate?

A

the molecule the enzyme breaks down

39
Q

what are proteins?

A

long chains of amino acids

40
Q

why does temperature increase the rate of reaction of an enzyme?

A

as the temperature increases, the enzyme and substrate are moving faster so there are more collisions per second between the enzyme and the substrate

41
Q

what are buffer solutions used for?

A

to control the pH of a solution in investigations

42
Q

what is the positive result of the ethanol test?

A

the solution goes from clear to a white cloudy emulsion

43
Q

what are the adaptations of the small intestine?

A

very long - provides a very large surface area for absorption
villi - increases surface area
microvilli - increase surface area further
good blood supply - increases concentration gradient
villi have thin membrane - ensures short diffusion path