B1 - Cell Biology Flashcards

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1
Q

What are both Animal and Plant cells?

A

Eukaryotes

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2
Q

What are Eukaryotic cells?

A

Cells that contain their genetic material (DNA) enclosed in a nucleus.

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3
Q

What are Features all Eukaryotic cells have?

A

Cell membrane
Cytoplasm
Nucleus
Genetic Material

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4
Q

What is a Prokaryotic Cell and Give an example?

A

In Prokaryotic cells, the genetic material (DNA) is not enclosed in a nucleus. e.g. Bacteria

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5
Q

What are the Differences between Prokaryotes and Eukaryotes?

A
  • Prokaryotes do not have mitochondria
  • Prokaryotic cells are smaller than Eukaryotes
  • Prokaryotes divide using binary fission while Eukaryotes use Mitosis
    Prokaryotes
  • Eukaryotes DNA is in a nucleus while Prokaryotes have DNA as a single molecule floating with additional plasmids
  • Prokaryotes are surrounded by a cell wall while Eukaryotes are surrounded by cell wall in plants and fungi
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6
Q

What is the Formula for Magnification?

A

magnification = image size/real size

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7
Q

What does 1 order of magnitude mean?

A

10 Times

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8
Q

What is the function of the Nucleus?

A

Contains genetic material, including DNA, which controls the cell’s activities.

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9
Q

What is the Cytoplasm and its function?

A

A jelly-like material that contains dissolved nutrients and salts and structures called organelles. It is where many of the chemical reactions happen like respiration.

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10
Q

What is the cell membrane and its function?

A

Its structure is permeable to some substances but not to others.

It therefore controls the movement of substances in and out of the cell.

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11
Q

Why can’t we see Mitochondria or Ribosomes?

A

They are too small to be seen using a light microscope. They can only be seen using an Electron Microscope.

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12
Q

What is the Mitochondria and its function?

A

Organelles that contain the enzymes for respiration, and where most energy is released in respiration.

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13
Q

What is a Ribosome and what do they do?

A

Tiny structures where protein synthesis occurs.

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14
Q

Draw an Animal cell and label it

A
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15
Q

Draw and Label a Plant cell

A
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16
Q

What are the three additional structures plant cells have over animal cells?

A

Chloroplast

Cell wall

Permanent vacuole

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17
Q

What is a Chloroplast and it’s function?

A

Organelles that contains the green pigment, chlorophyll, which absorbs light energy for photosynthesis. Contains the enzymes needed for photosynthesis.

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18
Q

What is a Cell wall and it’s function?

A

Made from cellulose fibres and strengthens the cell and supports the plant.

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19
Q

What is a permanent vacuole and its function?

A

Filled with cell sap to help keep the cell turgid.

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20
Q

Can Animal cells have a vacuole?

A

Animal cells may also have vacuoles, but these are small and temporary. In animals, they are commonly used to store or transport substances.

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21
Q

What does it mean if most animal and planat cells are specialised?

A

They have adaptations which help them to carry our their particular function.
When cells become specialised, scientists call that differentiation.

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22
Q

What is the job of the sperm cell?

How are they adapted to this?

A

Its job is to join with an ovum(egg cell). We call this Fertilisation. During Fertilisation the genetic information of the ovum and sperm combine.

  • The head of the sperm contains the genetic material for fertilisation.
  • The acrosome in the head contains enzymes so that the sperm can penetrate an egg.
  • The middle piece is packed with mitochondria to release energy needed to swim and fertilise the egg.
  • The tail enables the sperm to swim.
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23
Q

What is the job of the Nerve cell?
How is it adapted to it’s function?

A

The Nerve cell’s job is to send electrical impulses around the body.

  • The nerve cell is extended, so that nerves can run to and from different parts of the body to the central nervous system.
  • Dendrites increase the surface area so other nerve cell can connect more easily.
  • The end of the Axon has synapses which are junctions that allow the impulse to pass from one nerve to another.
  • The nerve cell is covered with a fatty sheath called Myelin, which insulates the nerve cell and speeds up the nerve impulse
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24
Q

What is the job of a muscle cell and how is it adapted to its function?

A

Their job is to contract

  • Muscle cells contain filaments of protein that slide over each other to cause muscle contraction.
  • They contain many well-developed mitochondria to provide the energy for muscle contraction.
  • In skeletal muscle, the cells merge so that the muscle fibres contract in unison.
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25
Q

What is the job of a root hair cell and how is it adapted to its function?

A

Their job is to absorb water and dissolved minerals.

  • It achieves this by having a increased surface area.
  • It does not have chloroplasts as it is underground.
  • It has thin walls so as not to restrict the movement of water.
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26
Q

What is the job of a xylem cell and how is it adapted to its function?

A

They form long tubes which carry water and dissolved minerals from the roots to the leaves.

They achieve this by:

  • very thick walls containing lignin. This provides support to the plant. But because the cell walls are sealed with lignin this causes the xylem cells to die.
  • There are no top and bottom walls between xylem vessels, so there is a continuous column of water running through them flowing more easily.
  • They have no nucleus, cytoplasm, vacuole or chloroplasts which makes it easier for water and minerals to flow.
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27
Q

What is the job of a Phloem cell and how is it adapted to its function?

A

Phloem tubes carry dissolved sugars up and down the plant.

They have two different types of cells:

    • Phloem cells which have no nucleus and only limited cytoplasm. The end walls of the vessel cells have pores called sieve plates which allow dissolved sugars to move cell interior.
    • Each Phloem Vessel cell has a companion cell connected by pores. Adjacent to the sieve tubes they provide energy required to transport substances in the phloem
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28
Q

What is the problem with light microscopes?

A
  • They have a limited magnification.
  • They have a limited resolution which means we cannot see fine details.
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29
Q

What is resolution?

A

the shortest distance between two points on a specimen that can still be seen as two different points by the observer.

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30
Q

What is an Electron Microscope and its advantages?

A

Electron microscopes use a beam of electrons instead of light rays.

There are two types of electron microscope:

The scanning electron microscope (SEM) has a large field of view so can be used to examine the surface structure of specimens. SEMs are often used at lower magnifications.
The transmission electron microscope (TEM) is used to examine thin slices or sections of cells or tissues.

  • Higher Magnification
  • Higher Resolution
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31
Q

What is Binary Fission?

A

A form of cell division used by prokaryotes to reproduce which is a simple form of cell division.

The bacteria splits into two bacterial cells

This level of replication will depend on the availability of nutrients and other suitable conditions such as temperature.

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32
Q

How do you calculate the number of bacterium produced in a certain amount of time?

Example Question) Under ideal conditions, a type of bacterium divides every twenty minutes. Calculate the number of bacteria present after three hours.

A

3 Hours = 180 minutes
180/20 = 9 rounds
2^9 = 512 = Number of Bacteria
or 5.12*10^2

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33
Q

What do we find in the Nucleus

A

We find Chromosomes that carry genetic information in a molecule called DNA.

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34
Q

What is a Gene?

A

a small section of DNA on a chromosome, that codes for a particular sequence of amino acids , to make a specific protein .

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35
Q

How many Chromosomes does each body cell contain?
How many pairs of chromosomes do each human body cells contain?

A

Each body cell contains two of each chromosome. They are paired

Human body cells contain 23 pairs of Chromosome.

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36
Q

What is the Female and Male Chromosome?

A

Each human body cell contains 46 chromosomes. These can be arranged into 23 pairs.

Each chromosome in a pair carries the same types of genes. The 23rd pair are the sex chromosomes:

In females, the two chromosomes are identical in shape. There are two X chromosomes. Females are referred to as XX.
In males, one of the chromosomes is a different in shape. There is an X and also a Y chromosome. Males are referred to as XY

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37
Q

What is the cell cycle?

A

A growing and dividing cell goes through a series of stages called the cell cycle.

38
Q

What are the stages of the Cell Cycle?

A

In the first stage(GROWTH STAGE) - , the DNA replicates to form two copies of each Chromosome. The cell also grows and copies its internal structures such as mitochondria and ribosomes.

In the second Stage - Mitosis takes place. One set of Chromosomes is pulled to each end of the cell. The nucleus also divides.

In the Final stage(Cytokinesis) of the cell cycle, the cytoplasm and the cell membrane divide to form two identical cells.

39
Q

What is the Process of Mitosis?

A

For a multicellular organism to grow, cells must divide by mitosis to produce two new identical daughter cells

During mitosis, one chromosome from each set is pulled to each end of the cell, and a new nucleus forms around each group of chromosomes

After this, the cytoplasm and cell membranes divide in a process known as cytokinesis. This results in the formation of two genetically identical daughter cells

40
Q

What happens to Chromosome during Mitosis?

A

So, if a human cell with 46 chromosomes in 23 pairs needs to divide by mitosis:
Its 46 chromosomes are doubled, so that there are two copies of each of the 46 chromosomes (or 92 chromatids) in total in the cell

The number of ribosomes and mitochondria subcellular structures also increases

During mitosis, the chromatids are pulled apart, with a complete set of 46 chromosomes in 23 pairs moving to each end of the cell

The cytoplasm and cell membrane divide, forming two new daughter cells

41
Q

What is the Function of Mitosis?

A
  1. Mitosis is essential for Growth and Development for multicellular organisms.
  2. Mitosis takes place when an organism repairs itself.
  3. Mitosis happens during Asexual Reproduction.
42
Q

How do you calculate how many cells are in each stage of the cell cycle to estimate how long each stage of the cell cycle lasts

A
43
Q

What is Differentiation?

A

Multicellular organisms are organisms made from more than one cell
You (a human) are multicellular, being made from trillions of cells

Specialised cells have a particular structure and composition that allow them to perform a specific function, and they form through a process known as differentiation
There are about 250 specialised cell types in the human body (e.g. red blood cell, motor neurone cell etc.)

If a specialised cell is able to divide by mitosis, the daughter cells produced will be the same type of specialised cell. These cells have lost their ability to differentiate into other types of cell

44
Q

What are Stem cells?

A

A stem cell is an undifferentiated cell of an organism which is capable of dividing (giving rise) to many more cells of the same type (undifferentiated stem cells)

From these cells, other cells can arise through the process of differentiation

45
Q

What are Embryonic Stem cells?

What are it’s Advantages and Disadvantages?

A

An embryo develops from a fertilised egg. Cells at the early stages in the development of the embryo are stem cells.

If cells are removed from the embryo – called embryonic stem cells - they will differentiate into any cell type however they have to be taken from a embryo which can be seen as unethical.

46
Q

What are Adult Stem Cells and where can they be found?

A

A type of stem cell found in specific locations in adults. Adult stem cells can only differentiate into a limited number of related cell types.

Adult stem cells can be found in several regions of the body, including the:

brain
eyes
blood
heart
liver
bone marrow
skin
muscles

Adult stem cells can differentiate into related cell types only, for example, bone marrow cells can differentiate into blood cells and cells of the immune system but not other cell types.

47
Q

Where are stem cells found in plants and how is it different to Animals.

A

Cell division in plants occurs in regions called meristems.

Cells of the meristem can differentiate to produce all types of plant cells at any time during the life of the plant.

The main meristems are close to the tip of the shoot, and the tip of the root.

In a growing shoot, new cells are being produced continuously near the tip. As the cells become older, further away from the tip, they become differentiated – they enlarge and develop vacuoles

48
Q

What are cells in the Circulatory system specialised to do?

A

Transport substances, defend the body, regulate temperature

49
Q

What are cells in the Excretory system specialised to do?

A

Remove waste products

regulate the water content of the body.

50
Q

What are cells in the Muscular system specialised to do?

A

Bring about movement

51
Q

What are cells in the Nervous system specialised to do?

A

Respond to internal and external stimuli and conditions, carry messages for the body work as a coordinated whole

52
Q

What are cells in the Respiratory System specialised to do?

A

Deliver oxygen for respiration and remove waste

53
Q

What are cells in the Reproductive system specialised to do?

A

Bring about fertilisation to produce new offspring

54
Q

What are cells in the Skeletal system specialised to do?

A

To bring about movement, support and protect internal structures, produce blood cells, store and release calcium

55
Q

What are cells in Palisade mesophyll specialised to do?

A

Carry out photosynthesis

56
Q

What are cells in Spongy mesophyll specialised to do?

A

Allow gases to circulate for the exchange of gases between the leaf and the environment, carry out some photosynthesis

57
Q

What are Guard cells specialised to do?

A

Open and close to control the exchange of gases – carbon dioxide, water vapour and oxygen

58
Q

What are sieve tubes specialised to do?

A

Transport products of photosynthesis, including sugars and amino acids, from the leaf to where they are needed

59
Q

What are Companion cells specialised to do?

A

Provide the energy required for transporting substances in sieve tubes

60
Q

What are Xylem vessels specialised to do?

A

Transport water and dissolved minerals from the roots, up the plant

61
Q

What are cells in the Meristem specialised to do?

A

Produce new cells as they divide

62
Q

How do we clone plant from meristem cells?

A

Cells from meristems can be cloned. Meristematic cells are removed from a plant and grown in tissue culture. The cells are grown in a culture medium that contains agar – to provide support and water for the growing cells – along with nutrients and plant hormones to stimulate growth and cell division.

63
Q

Why do we clone plants?

A

Plants are cloned to produce identical plants quickly and economically.

Producing new plants by cloning is quicker than allowing plants to reproduce and collecting and sowing seeds. It’s therefore an effective way of producing new individuals from rare and endangered plants, helping to preserve the species.

Clones will be genetically identical to the original plant providing the meristem cells. This is useful to provide crop plants for large-scale cultivation that have desirable characteristics such as disease resistance.

Unlike in animals, plant stem cells can be obtained easily, and without ethical objection, from meristems. They can then be used to produce clones of plants quickly and economically

Plant clones can be produced in weeks at a relatively low cost for their potential value
Rare species at risk of extinction as a result of human activity can be cloned to protect them and maintain biodiversity

The plants produced are clones, so although numbers increase, genetic diversity within clones is low

Cloning rare plants gives scientists more time to study them to see if they can produce any compounds of interest

Crop plants with special features such as disease or pest resistance can be cloned to produce large numbers of identical plants for farmers
This reduces the need to use chemicals such as pesticides, which has environmental benefits

64
Q

Why are stem cells useful in medicine

A

Stem cells can divide to produce new cells, which can then divide into different cell types. They therefore have the potential to be transplanted into patients to treat medical conditions and disease. They could be used to replace cells that have been damaged or destroyed, eg:

  • type 1 diabetes
  • multiple sclerosis, which can lead to paralysis
  • spinal cord or brain injury, that have led to paralysis
65
Q

How can stem cells treat type 1 Diabetes and why?

A

As there is an inability on the pancreas to produce insulin to regulate blood sugar levels as a result Stem cells can differentiate into pancreatic cells and transported into the patient which produces insulin and as a result regulate blood sugar levels.

They have to be sourced from Stem cell donors, embryos or through therapeutic cloning.

66
Q

How and why can stem cells treat paralysis

A
67
Q

What can Adult stem cells be used for medicine?

A

Adult stem cells will differentiate into a narrower range of cell types. Bone marrow transplants are an example of adult stem cell transplant. Bone marrow cells will differentiate into different types of blood cell. Bone marrow transplants are carried out:

  • in cases of blood cell cancer such as leukaemia and lymphoma
  • when blood cells have been destroyed by cancer treatment
68
Q

What are advantages with Adult stem cells?

A

Adult stem celltransplants use a patient’s own stem cells. They are therefore genetically identical and will not be rejected by the patient’s immune system.

There are also fewer complications if the stem cells come from a close member of the family. Alternatively, donor cells can come from a person with closely-matched tissue types.

Embryonic stem cells will always come from a donor – unless stem cells were collected from the patient as an embryo.

69
Q

What is Therapeutic cloning and how does it take place?
What are it’s advantages?

A

Therapeutic cloning could produce stem cells with the same genetic make-up as the patient.

The technique involves the transfer of the nucleus from a cell of the patient, to an egg cell whose nucleus has been removed.

Stem cells produced in this way could be transferred to the patient. Although human stem cells have been produced in this way, and used in research, there is no evidence that, as yet, they have been used to treat anyone.

70
Q

What are clinical issues with Stem cells?

A
  • There is no guarantee how successful these therapies will be, for example the use of stem cells in replacing nerve cells lost in Parkinson’s disease patients.
  • difficulty in finding donors.
  • difficulty in obtaining and storing a patient’s embryonic stem cells.
  • Mutations have been observed in stem cells
  • Cultured stem cells could be contaminated with viruses which would be transferred to a patient.
71
Q

What are Ethical issues with Stem cells?

A
  • A source of embryonic stem cells is unused embryos produced by in vitro fertilisation (IVF)
  • For therapeutic cloning is it right to create embryos for therapy, and destroy them in the process?
  • Embryos could come to be viewed as a commodity, and not as an embryo that could develop into a person.
  • At what stage of its development should an embryo be regarded as, and treated as a person?
72
Q

What are Social issues with Stem cells?

A
  • Educating the public about what stem cells can, and can’t do, is important.
  • Whether the benefits of stem cell use outweigh the objections.
  • Much of the research is being carried out by commercial clinics, so reported successes are not subject to peer review. Patients could be exploited by paying for expensive treatments and being given false hope of a cure as stem cell therapies are only in their developmental stages.
73
Q

Benefits of Stem Cells

A
  • No need for Organ transplant
  • Could treat paralysis or Type 1 diabetes
  • There have been many successful trials
74
Q

What are the three transport processes?

A

There are three transport processes that living organisms use for exchange: diffusion, osmosis and active transport

75
Q

Explain what diffusion is

A

Diffusion is the spreading out of the particles of any substance in solution, or particles of a gas, resulting in a net movement of particles from an area of higher concentration to an area of lower concentration. It is a passive process as it requires no energy.

This means:
Particles in a solution or a gas are always moving about randomly
If there happen to be more particles in one area/region, then there tends to be an overall (net) movement of particles from this region of higher concentration to a region where there are fewer particles (an area of lower concentration)

76
Q

How does diffusion take place in living organisms?

A

All cells are surrounded by a cell membrane which separates the inside of the cell from its outside environment
Substances have to be able to diffuse through the cell membrane to enter or exit the cell unaided (there are other ways for substances to get in)
Not all substances are able to diffuse across the cell membrane which is why it is described as being ‘partially permeable’; some substances are able to enter or leave the cell whilst others aren’t
For example, oxygen and carbon dioxide can diffuse across the cell membrane during gas exchange whereas the carbohydrate starch cannot (it’s too big)

77
Q

Give examples of substances moving in and out of organisms using diffusion

A

Examples of substances that enter cells by diffusion:
-Oxygen must diffuse into cells for aerobic respiration

-Carbon dioxide must diffuse into photosynthesising plant cells to be available for photosynthesis

Examples of substances that leave cells by diffusion:
-Liver cells break down excess amino acids into the waste product urea which is highly toxic and must diffuse out of cells to be excreted by the kidneys

-Carbon dioxide produced by aerobic respiration must diffuse out of cells

78
Q

Why A photosynthesising leaf cell will likely have a lower carbon dioxide concentration on a bright day compared to its environment?

A

A photosynthesising leaf cell will likely have a lower carbon dioxide concentration on a bright day compared to its environment, as carbon dioxide is used in photosynthesis faster than it is produced in aerobic respiration. At night, when the plant is not photosynthesising the carbon dioxide concentration inside the same leaf cell will be higher than the environment as the leaf cell is always respiring.

79
Q

What are the three factors that affect the rate of diffusion and why?

A
  • Temperature
  • Surface Area
  • Concentration gradient
80
Q

How is the small intestine adapted for gas exchange?

A

Role in the body:
- Most absorption of digested food molecules into the bloodstream occurs across the wall of the small intestine.

How it’s adapted to its role:
- Huge surface area, produced by thousands of villi.

  • Only one layer of epithelial cells covers the surface of each villus – this decreases diffusion distance
  • Each villus has a good blood supply – this maintains a concentration gradient
81
Q

How are the Alveoli adapted for gas exchange?

A

The alveolus is the gas exchange surface in humans

Role in the body:
- Gas exchange between air in the alveoli and the blood, to supply cells with oxygen for aerobic respiration and to remove carbon dioxide

How it’s adapted to its role:

  • Millions of alveoli (singular: alveolus) which collectively provide a huge surface area this increases SA
  • The wall of each alveolus is one cell thick, with a moist lining and excellent blood supply - this maintains a concentration gradient
  • Extensive blood supply - ensuring oxygen rich blood is taken away from the lungs and carbon dioxide rich blood is taken to the lungs.
82
Q

How are gills adapted to gas exchange?

A

-Each gill is made from lots of smaller plates called filaments, which themselves are covered in projections called lamellae – this increases SA

-Dense capillary network ensures a good blood supply which flows in the opposite direction to water passing through the gills – this maintains a concentration gradient

83
Q

How are Roots adapted to gas exchange?

A

Role in the plant:
- To absorb water and mineral ions (such as magnesium and nitrate ions) from the soil and anchor the plant

How it’s adapted to its role:

  • Mitochondria for active transport of mineral ions
  • The surface of the roots are covered in root hair cells – this increases SA
  • Thinner walls (due to shorter diffusion distance)
  • Permanent vacuole contains cell sap which is more concentrated than soil water, maintaining a water potential gradient
  • Remember that chloroplasts are not found in these cells – there’s no light for photosynthesis underground!
  • The root network is highly branched - this increases SA
84
Q

How is the Leaf adapted for gas exchange?

A

Role in the plant:

  • The leaves contain most of a plant’s photosynthetic cells
  • Photosynthesis requires efficient gas exchange between air surrounding the leaf and the photosynthetic cells, whilst minimising water loss

How it’s adapted to its role:

  • Stomata are tiny openings that predominantly cover the lower side of the leaf, allowing air to circulate inside the leaf – this decreases diffusion distance for carbon dioxide and oxygen
  • The lower layer of the leaf is made from spongy mesophyll cells which allow air to circulate inside the leaf
85
Q

What is Osmosis?

A

Water moves from a high water to a low water concentration across a partially permeable membrane and down a concentration gradient

Osmosis is a just a special type of diffusion, so water molecules move about randomly just like any other particle that can diffuse

Water will always move from a more dilute solution to a more (solute) concentrated solution to even up the concentration of water molecules on each side of the membrane

86
Q

What does dilute and concentrated mean?

A
87
Q

What is the Effect of Osmosis on Plant Tissue

A

The movement of water by osmosis can easily be observed by placing two similar pieces of plant tissue into solutions with differing concentrations

If the plant tissue gains mass:

  • *- Water must have moved into the plant tissue from the solution surrounding it by osmosis
  • The solution surrounding the tissue is more dilute than the plant tissue (which is more concentrated)**

If plant tissue loses mass:

  • *- Water must have moved out of the plant tissue into the solution surrounding it by osmosis
  • The solution surrounding the tissue is more concentrated than the plant tissue (which is more dilute)**

If there is no overall change in mass:

  • There has been no net movement of water as the concentration in both the plant tissue and the solution surrounding it must be equal
  • Remember that water will still be moving into and out of the plant tissue, but there wouldn’t be any net movement in this case
88
Q

What is Active Transport?

A

Diffusion and osmosis rely upon the passive transport of substances down concentration gradients, relying on the random movement of particles
However, cells also need to be able to transport substances across the cell membrane against a concentration gradient. This requires energy released by respiration
The energy from respiration is used by protein transport molecules embedded in the cell membrane to move substances into or out of the cell
Active transport moves substances from a more dilute solution to a more concentrated solution

89
Q

What is Active Transport?

A

Diffusion and osmosis rely upon the passive transport of substances down concentration gradients, relying on the random movement of particles
However, cells also need to be able to transport substances across the cell membrane against a concentration gradient. This requires energy released by respiration
The energy from respiration is used by protein transport molecules embedded in the cell membrane to move substances into or out of the cell
Active transport moves substances from a more dilute solution to a more concentrated solution

90
Q

Give an example of active transport in plants and animals

A

Plants

  • Root hair cells lining the surface of plant roots need to move minerals such as magnesium ions from a region of lower concentration (the very dilute solution of minerals in the soil surrounding the roots) to a region of higher concentration (inside the cytoplasm of the cell)
  • Mineral ions are needed by plants to function healthily.
  • Magnesium ions are needed to make chlorophyll
  • Nitrate ions are needed to make amino acids and therefore for protein synthesis (and subsequently growth)

Animals
- Food molecules (such as the sugar glucose) can be absorbed across the wall of the small intestine by diffusion, but this is dependent on a concentration gradient existing between the lumen of the intestine and the bloodstream
- Active transport allows sugar such as glucose to be transported into the bloodstream from the lumen of the small intestine (the gut) when the concentration of sugar molecules in the blood is higher
Sugar molecules are used in respiration to release energy for cells to function

91
Q

Compare Transport Processes

A