B1 Flashcards
Who and when first described cells
1665 Robert Hooke
Who and when was the first living cell absorbed
1674-1683 Anton van Leeuwenhoek first to observe bacteria and protoctista from pond water samples
Who and when was the first person to observe a nucleus
1831 and Robert Brown
Who created the universal theory?
What is it?
Who made it?
1839-Matthias Schleiden
All plant materials is composed of cells
The scientist credited for theory is Theodor Schwann-he proposed all living things composed of cells
Who and when was spontaneous generation disproved
1861-Louis Pasteur
He disproved as he demonstrated that bacteria will only grow in sterile nutrient broth after exposed to air
Who and when demonstrated evidence for the origin of new cells
1852-Robert Ramek observed cell division in animal cells-findings were not accepted at the time
Rudolf Virchow published the findings as his own to show new cells form from existing ones
When were light microscopes first developed
16th century
Limitations of light microscopy
Lower magnification and resolution compared to others
Maximum magnification of a light microscope is ×1500 +resolution is 200nm
Benefits of light microscope
Can observe sub-cellular structures/organelles e.g nucleus, mitochondria and chloroplasts
Resolution definition
The ability to distinguish between objects that are close together
How do electron microscopy work
Use a beam of electrons in a vacuum with a wavelength of less than 1nm to visualise specimen
Advantages of electronic miscroscopy
See more detail
Produce images called electron micrograph
Magnification of ×500,000
Higher resolution of 0.1nm
More stains can be used other than methylene blue which is only used for light microscopy
Magnification calculation
Size of image/actual image
How many nanometers is in a micrometre
1000
How many micrometers is in a millimetre
1000
How many millimetres is in a metre
1000
What is a prokaryotic cell
Single-celled organisms
Much smaller than eukaryotic
Bacteria cells
DNA is not contained in a nucleus
What is in a prokaryotic cell
Cell wall, capsule, ribosomes,nucleoid and plasmid
Cell wall structure and definition
Made of peptidoglycan +protects and supports cell
Capsule
Slippery layer outside cell wall of some species of bacteria+
Protects the cell and prevents dessication
Ribsomes
Smaller than ones in eukaryotic
Consist of two subunits and not surrounded by membrane
Nucleoid
Irregular shape that holds nuclear material without a nuclear membrane + holds genetic material
The Dna forms one circular chromosome
It is where genetic info can be found and controls cells activity
Plasmid
Small loops of DNA
They carry genes that benefit the survival of organism
Function of bacterial cells
Transcription proceess- RNA is formed (molecule with long chain of nucleotides) as it is paired with DNA containing a genetic code and when paired the hydrogen bond breaks
What is the practical for bacterial cells
Gram stain
What does gram stain identify
Bacteria that causes infections to enable them to decide most effective treatment