Automated Hematology

Question 1

Name two automated cell counting technologies

Answer 1

Electrical impedance and light scatter

Question 2

Give an example of an electrical impedance instrument and light scatter instrument

Answer 2

EI- Beckmann coulter onyx, Beckmann coulter act diff2, serono baker

LS- Beckmann coulter vcs, stks, gen-s, LH 755, sysmex XT 2000i

Both - abbott cell dyno 4000

Question 3

Identify and describe common interfering substances that may affect the reliability of automated results

Answer 3

WBC, RBC, HGB, HCT, PLT. See notes

Question 4

Discuss the operation and maintenance associated with automated hematology analyzers

Answer 4

Maintenance procedures outlined in manufacturers manual. Preventative maintenance. Procedures should be documented carefully. See notes

Question 5

Compare the accuracy and precision of manual and automated blood cell counting methods

Answer 5

The automated measurements are more accurate and precise than manual. Manila counts are plus/minus 10%. Automated plus/minus 1-2%