automated

Question 1

What is the operating temperature range?

Answer 1

10 to -30

Question 2

What is the optimum working temperature range?

Answer 2

-18 to -20

Question 3

At what temperature should fatty tissue, skin with fatty subcutis, fatty breast, and omental tissue be kept?

Answer 3

-35

Question 4

What is the appropriate temperature range for brain, lymph nodes, liver, spleen, kidney, testis, uterine curettings, soft cellular tumor, and thyroid tissue?

Answer 4

-5 to -15

Question 5

What temperature range should muscle, connective tissue, pancreas, uterus, cervix, non-fatty skin, non-fatty breast tissue, ovary, prostate, tongue, and gut be kept at?

Answer 5

-15 to -25

Question 6

What freezing methods are used for tissue preservation?

Answer 6

Liquid nitrogen, isopentane, CO2, aerosol spray

Question 7

For what purposes is liquid nitrogen used in tissue freezing?

Answer 7

For histochemistry and intraoperative procedures

Question 8

What should be allowed to equilibrate when using liquid nitrogen for freezing tissues?

Answer 8

The tissue

Question 9

What are some mounting media used for tissue sections?

Answer 9

Water, 20 to 30% bovine albumin, Von Apathy gum syrup, synthetic water-soluble glycols and resins

Question 10

What procedure should be followed for fresh unfixed tissue sections?

Answer 10

Wave for a few seconds, or fix immediately in formol-alcohol (15 + 85 ml)

Question 11

What should be used for mounting formalinized/fixed tissues?

Answer 11

Albumin or Zwemer’s chrome-glycerin jelly

Question 12

Where should the mounted section be placed after mounting?

Answer 12

In a covered coplin jar containing 40% formaldehyde for 1 to 5 minutes

Question 13

What should be done to the microtome for proper operation and maintenance?

Answer 13

Leave ON at all times and apply special low-temperature oil