AUBF LAB - FINSLS Flashcards
16 Macroscopic Stool Characteristics
Color / Appearance
Black
Red
Pale, Yellow, White, Gray
Green
Bulky/frothy
Ribbon-like
Mucus/Blood-streaked mucus
16 Macroscopic Stool Possible Causes B R P G B R M
Upper GI bleeding Iron therapy Charcoal Bismuths (antacids) ---------------- Lower GI bleeding Beets & food coloring Rifampin ------------------ Bile-duct obstruction Barium sulfate ------------------ Biliverdin/Oral antibiotics Green Veggies ------------------- BD obstruction Pancreatic disorder -------------- Intestinal constriction -------------- Colitis Dysentery Malignancy Constipation
16 Stool Consistency
Formed Semi-formed Soft Watery Mucoid
17 Fecal occult blood
produce a black, tarry stool
BLEEDING IN THE UPPER GIT
17 Fecal occult blood
result in an overly bloody
BLEEDING IN THE LOWER GIT
17 Fecal occult blood
meat free diet for _ days
produce false positive blood test
4 days
Salmon,
Sardines,
Mutton
17 Fecal occult blood
PROCEDURE - RESULTS
BENZIDINE TEST - BLUE TO GREEN
GUAIAC TEST - BLUE TO GREEN
18 FECAL UROBILIN
Increases the amount of urobilinogen
increased destruction of RBC (hemolytic anemia)
18 FECAL UROBILIN
reduces the flow of bilirubin in the intestine and decreases the fecal excretion of urobilinogen
Liver dse
18 FECAL UROBILIN
reduces the flow urobilinog to very low levels
complete obstruction of BD
18 FECAL UROBILIN
this test investigates __ and ___ conditions
hemolytic disease and hepatic obs. conditions
18 FECAL UROBILIN
Reference values
50-300mg/24hrs or 100-400Ehrlich units/100g
Newborns - 6months (-)
18 FECAL UROBILIN
PROCEDURE - RESULT
Schmidt’s test
Urobilin (normally) - red
Bile (not normal) - green
19 MICROCOSPIC EXAMINATION OF FECES
detect the presence of ___ assoc with ___ ___ and ___ __ __ and __ assoc with ___
leukocyte
microbial diarrhea
undigested muscle fibers and fats
19 MICROCOSPIC EXAMINATION OF FECES
reagent:
NSS
19 MICROCOSPIC EXAMINATION OF FECES
Fecal leukocyte primarily __
seen in___
neutrophils
conditions that affect the intestinal mucosa
1) Ulcerative colitis
2) bacterial dysentery
19 MICROCOSPIC EXAMINATION OF FECES
_ _ _ can be helpful in the dx and monitoring the patients with __ __ such as in cases of __ ___
Undigested striated muscle fibers
pancreatic insufficiency
cystic fibrosis
19 MICROCOSPIC EXAMINATION OF FECES
PROCEDURE - RESULT
Direct fecal smear - motile stages in fresh stools (cyst) Lugol’s
20 MACROSCOPIC EXAMINATION OF CSF
__ is a clear, colorless fluid formed within the cavities (ventricles of the brain)
CSF
20 MACROSCOPIC EXAMINATION OF CSF
The _____ produces about 70% of the CSF by ___ and ___
choroid plexus
ultrafiltration and secretion
20 MACROSCOPIC EXAMINATION OF CSF
The ___ ___ of the ventricles and ___ __ ___ produce the remainder of the CSF total volume.
ependymal lining
cerebral subarachnoid space
20 MACROSCOPIC EXAMINATION OF CSF
Approximately __ of CSF fluid is formed per day, although only __ to __ is present in the system at any one time.
500mL
90 to 150mL
20 MACROSCOPIC EXAMINATION OF CSF
Reabsorption of CSF occurs at the ___ __
arachnoid villi
20 MACROSCOPIC EXAMINATION OF CSF
The initial appearance of the normally ______ can provide valuable diagnostic information
crystal clear CSF
20 MACROSCOPIC EXAMINATION OF CSF
Appearance–
Crystal, clear
Hazy, turbid, cloudy,
milky
Oily
Bloody
Xanthochromic
Clotted
Pellicle
20 MACROSCOPIC EXAMINATION OF CSF
Cause–
--- WBCs RBCs Microorganisms, Protein - Radiographic contrast media - RBCs - Hemoglobin Bilirubin Carotene Protein Melanin - Protein Clotting factors - Protein Clotting factors
20 MACROSCOPIC EXAMINATION OF CSF
Normal
- -Meningitis, Hemorrhage.
- -Traumatic tap, disorders that affect BBB, production of IgG within the CNS
–Hemorrhage, Traumatic Tap
- -Old hemorrhage
- -Lysed cells from tap
- -RBC degradation
- -Elevated serum bilirubin levels
- -Increased carotene blood levels
- -Meningeal sarcoma
–Traumatic tap
- -Disorders that affect the blood-brain barrier
- -Tubercular meningitis
21 CHEMICAL DETERMINATION OF PROTEIN CSF
Reagents:
Reagents:
phenol crystals,
distilled water,
sat. aqueous solution of phenol, ammonium sulfate, conc. HCL, 2% solution of formaldehyde, 0.06% solution of Sodium nitrate
21 CHEMICAL DETERMINATION OF PROTEIN CSF
Protein concentration normally increases from the __ to the ___ and finally to the ____
Protein concentration
ventricles
cisterns
lumbar sac
21 CHEMICAL DETERMINATION OF PROTEIN CSF
The CSF protein is a nonspecific but reliable indication of CNS pathology such as __ __ __ and other ___ causing ____
meningitis, brain abscess, MS,
degenerative processes causing neoplastic disease.
21 CHEMICAL DETERMINATION OF PROTEIN CSF
Elevated csf levels may be caused by 4
- increased permeability of the BBB
- decreased resorption of the arachnoid villi
- mech. obstruction of the CSF flow
- increased intrathecal immunologic synth
21 CHEMICAL DETERMINATION OF PROTEIN CSF
Normal RV
Adults: 15-45mg/dL
15-25mg/dl
5-15mg/dl
Neonates: 15-100mg/dl
Elderly: (>60yo): 15-60mg/dl
21 CHEMICAL DETERMINATION OF PROTEIN CSF
PROCEDURE
Pandy’s test - bluish white cloud
Ross-Jones test
1+ = thin white ring at zone of contact (disappears)
4+ = heavy cloud (not disappear)
Tryptophan
22 CHEM DET OF GLUCOSE IN CSF (Qualitative)
Reagents:
Benedict’s qualitative rgt
Distilled water
22 CHEM DET OF GLUCOSE IN CSF
The CSF glucose level varies
with the blood glucose levels.. It is usually about _ of BG lvl
_ min
60%
60min
22 CHEM DET OF GLUCOSE IN CSF helpful in det 3
impaired transport of glucose from plasma to CSF,
INC USE OF Glucose in the CSF,
glucose utilization by leukocytes and m.o
22 CHEM DET OF CSF GLUCOSE
Markedly dec CSF glu accompanied by an inc wbc count with a large percentage of neutro is indc of
BACTERIAL MENINGITIS
22 CHEM DET OF CSF GLUCOSE
NRV
Adult 40-7mgdl or 2.2-3.9mmol/l
Child 60-80 or 3.3 to 4.4
CSF-to-plasma glucose ratio <0.5
CSF glucose lvl 60-70% BGL
22 CHEM DET OF CSF GLUCOSE
Procedure
Benedicts test
Normal sugar - turbid greenish yellow
Abs of sugar (patho) - no color change
excess of prot but no sugar-deep purplish violet or pinkish-violet color
23 MICRO EXAM OF CSF
Rgts
wright’s stain
methanol
23 MICRO EXAM OF CSF Normal csf contains _ and _
ratio of _
lympho and mono
70:30 in adults
23 MICRO EXAM OF CSF a higher proportion of _ is present in young children
monocytes
23 MICRO EXAM OF CSF
inc WBC
pleocytosis
23 MICRO EXAM OF CSF
elevated WBC
inflam dse
hemorrhage
neoplasms
trauma
23 MICRO EXAM OF CSF
NRV
adult 0-5wbc/ul or 5x10^6wbc/L
nb - 0.30
child 0-15
23 MICRO EXAM OF CSF
L
M
P (neut)
40-80—5-35
15-45—50-90
0-6—0-8
23 MICRO EXAM OF CSF
PROCEDURE
total leuko count
23 MICRO EXAM OF CSF
Princip
cells are counted by _
___are due to traumatic bleeding
manual couning chamber
bloody taps