AT- i

Question 1

Describe microbiological aseptic techniques

agar plate and broth

Answer 1

Bench or work surface has been thoroughly cleaned and disinfected

Light a bunsen burner and work near the lit Bunsen throughout the practical, the warm air current will create an updraft that reduces the risk of air-borne microbes contaminating your agar.

Use sterile equipment by autoclaving (heating at high temperature and pressure)- using bunsen burner

flame the neck of the bottle

open lid of Petri dish as little as possible

use sterile spreader to evenly distribute e.g bacteria