Application of Molecular Techniques in the Diagnosis IN GENETICS AND FORENSICS Flashcards
Genetics in Molecular Biology specifically deals with the?
Human Chromosome
This refers to the complete set of the chromosomes in the cells of organisms
Karyotyping
It is a new technique under molecular biology
In situ Hybridization
This technique before uses radiolabeled probes as target labels
In Situ Hybridization
This is a tagging technique in FISH
Nick Translation
It is a method on how we add the probe into the sample or reagent
Nick Translation
It is a process wherein the DNA polymerase I is used to replaced some nucleotides of DNA sequences
Nick Translation
Give the advanced application of FISH
- Interphase FISH
- WCP (Whole Chromosome Painting
- MFISH (Multicolor FISH)
- SKY (Spectral Karyotyping)
- ALL-TELOMERE0FISH
- Comparative Genomic Hybridization
There is no cell culture required here, thus the diagnosis is much rapid
Interphase FISH
In this application, different or many types of probes are used from a SINGLE chromosome
WCP (Whole Chromosome Painting)
The main principle of this application is, on the single chromosome, there are LOTS OF UNIQUE DNA associated with it
WCP (Whole Chromosome Painting)
The main advantage of this application is that it is much more automated and fixed profile
MFISH (Multiple FISH)
This application is a computer based
MFISH (Multiple FISH)
In this type of application, you will be able to observe multiple chromosomal aberrations or changes
SKY (Spectral Karyotyping)
This application is almost the same with WCP, but instead of a single chromosome, multiple chromosomal aberrations are detected
SKY (Spectral Karyotyping)
The main probe/label of this application is a telomere probe
ALL-Telomere FISH
This probe is used to recognize six base repeats which is present at the end of all choromosomes
Telomere probe
This application detects six base repeats sequence present at the ends of all chromosome
ALL-Telomere FISH
The main advantage of this application is used as a confirmatory assay
ALL-Telomere FISH
In this type of application, you simply compare the DNA content of a differentially labeled normal and abnormal cell population
Comparative Genomic Hybridization
In this application, we only compare DNA content
Comparative Genomic Hybridization
Give the main application of FISH
- Diagnosis
- Prognosis
- Evaluate
It refers to some kind of the inherited diseases associated with mutation
Genetics
It is a certain condition whether there is a presence or absence of a mutated gene
Prognosis
Advantages of FISH
- much more specific
- distinguish one disease from another
- detected disease more easily
- can be quantitated through automated methods
It is one of the most powerful tools in the fight against crime and other legal matters
DNA Testing
It is a common specimen that can be used in identifying criminal suspects in forensic
Hair
Age, sex, and sometimes medical history can be determine by this
bones
This evidence can be used in police and court matters
Fingerprint
This is considered as a best sample to use in forensics
Blood
Where can we obtain DNA?A
It can be obtain from blood, semen, saliva, bone, teeth, or microscopic flecks of skin
The highest amount of DNA can be obtained in?
Male semen
It is the best specimen if one wants to investigate crime associated with sexual assault
Semen
True or False:
Forensics may also be used in identifying issues in relation to that of the family
True
How will you know if a certain sequence is a Satellite DNA?
As long as there are two repeating sequences
