Aplication Of DNA Tech Flashcards
1
Q
What is DNA fingerprinting?
A
-DNA fingerprinting is a laboratory technique used to determine the probable identity of a person based on the nucleotide sequences of certain regions of human DNA that are unique to individuals
2
Q
How is the nucleotide sequence used in DNA fingerprinting arranged?
A
- it is arranged in repeating sequences of base pairs (STRs - Short Tandem Repeats)
3
Q
What type of DNA is used in DNA fingerprinting?
A
- the non-coding DNA is used as it differs from person to person
4
Q
What are the uses in DNA profiling? (5)
A
- to identify crime suspects in forensic investigations
- as proof of paternity
- to determine the probability and cause of genetic defects
- to trace missing persons
- to establish the compatibility of tissue types for organ transplants
5
Q
What is PCR?
A
- Polymerase Chain Reaction
6
Q
What does PCR do?
A
- PCR replicates DNA because evidence samples often do not contain enough DNA for testing.
- It can make millions of DNA segments from just a few copies of sample DNA
7
Q
How does PCR replicate DNA segements?
A
- Through cycles of heating and cooling and using enzymes and nucleotides, the DNA fragments are replicated
8
Q
What is the importance of PCR?
A
- is important in DNA fingerprinting as it ensures that there is enough DNA for testing
9
Q
What is Gel Electrophoresis?
A
Gel Electrophoresis is a method that separates Nucleic acids according to their size
