Antibody Detection

Question 1

What is the focus of antibody detection methods?

Answer 1

Irregular/Unexpected antibodies
Immune Allantibodies

Question 2

What is produced in response to stimulation to RBC by transfusion, transplantation, or pregnancy?

Answer 2

Unexpected Antibodies

Question 3

What is produced in one individual and then transmitted to another? Give an example.

Answer 3

Passive (Acquired) Antibodies
Intravenous Immunoglobulin (IVIG)

Question 4

Who does the AABB require the use of an antibody screen for?

Answer 4

Pretransfusion
Prenatal Obstetric patients
Allogenic blood donors

Question 5

What are the Tube Method phases?

Answer 5

Immediate Spin (IS)
37 C incubation phase
AHG phase

Question 6

What doe we use check cells for?

Answer 6

Confirming negative tests

Question 7

How do we make Screen cells?

Answer 7

From Group O individuals

Question 8

What are the Enhancement Reagents we can use on antibody detection?

Answer 8

Albumin (Disperse the charges)
LISS (Increase the uptake of antibody)
Polyethylene Glycol (PEG) (Increases the degree of RBC sensitization)

Question 9

What are the advantages and disadvantages of the Gel Method on antibody screens?

Answer 9

Advantage: Standardized Pipetting, Reduced Specimen Volume
Disadvantage: Longer turnaround, Less sensitive, Need for incubators and centrifuges

Question 10

What are the advantages of the Solid Phase Adherence Method?

Answer 10

Automation
Small sample size

Question 11

What factors can effect sensitivity in identifying antibodies?

Answer 11

Cell to serum ratio
Temperature and reactivity phase
Incubation length
pH (6.8 - 7.2)