Antibodies

Question 1

Opsonisation

Answer 1

Bind bacteria to promote uptake by phagoctyes

Question 2

Agglutination

Answer 2

Clear cells more efficiently via phagocytes, fewer infectious units

Question 3

ADCC

Answer 3

Antibody dependent cell-mediated cytotoxicity. Bind target cell, recruits NK cells to lyse cell

Question 4

Inflammation

Answer 4

Disrupt cell, attracts phagocytes and other immune cells

Question 5

Neutralisation

Answer 5

Neutralise toxins e.g. diptheria

Blocks adhesion of microbes to mucosa

Question 6

Types of actions of antibodies

Answer 6

Complement, opsonisation, agglutination, inflammation, neutralisation, ADCC

Question 7

Complement

Answer 7

Activate via classical pathway

C1 binds IgG, C2 and CE cleaved by C1, c2a and c4b form c3 convertase

Question 8

Monomer Abs

Answer 8

IgA, IgG, IgD, IgE

Question 9

Dimer/Trimer Abs

Answer 9

IgA

Question 10

Pentamers

Answer 10

IgM

Question 11

Antibody reactinos key in which type of infection?

Answer 11

Bacterial

Question 12

Infections showing neutralisation, agglutination and complement

Answer 12

Pertussis, Strep. Pneumonia

Question 13

Infections showing toxin neutralisation

Answer 13

Diptheria, anthrax, tetanus

Question 14

Light Chains

Answer 14

Two identical, 22kda

Question 15

Heavy chains

Answer 15

Two identical, 55kda

Question 16

Fab - Antigen binding fragment

Answer 16

Hypervariable region, include CDRs and framework regions

Question 17

Fc - Crystallising fragment

Answer 17

Region determining effector functions

Question 18

Chimeric mAbs

Answer 18

Humanised - replace constant regions

Question 19

CDR grafted mAbs

Answer 19

Humanised - Replace complementarity determining regions

Question 20

Fully humanised

Answer 20

From transgenic mice