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Microbiology > antibodies > Flashcards

antibodies Flashcards

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1
Q

Ig G

A

main serum antibody
has less carbohydrates
half life 23 days
concentration dependant metabolism

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2
Q

Ig G

A

8-16 mg per ml

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3
Q

Ig G functions

A 
opsonization
cross placental
present ag to k cells
stimulate fc receptors on platelet
cft
ppt
neutralisation
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4
Q

Ig A

A

0.6-4.2 mg per ml

half life - 6-8 days

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5
Q

Ig A functions

A

phagocytosis
intracellular killing
in secretions

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6
Q

IgM

A

0.5-2mg per ml

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7
Q

IgM half life

A

5 days

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8
Q

J chains

A

Ig A and Ig M

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9
Q

extra CH present in

A

IgM

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10
Q

2 mercaptoethanol - 0.12 M

A

destroy IgM only

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11
Q

better neutralisation antibodies

A

IgM

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12
Q

IgM functions

A 
immune hemolysis
opsonization
bactericidal
agglutination
recognisation receptors on b cells
neutralisation
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13
Q

intravascular

A

IgM, IgD

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14
Q

IgD

A

3 mg per 100ml

recognition receptors on b lymphocytes - activation

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15
Q

IgE

A

homocytotropism
prausnitz-kustner reaction

susceptibility to merceptoethanol

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16
Q

multiple myeloma

A

bence jones proteins - light chain - either k or l - monoclonal
b cell proliferation - abnormal
Ig, G A D E

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17
Q

waldenstrom macroglobulinemia

A

excessive m proteins (IgM) and their bence Jones protein

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18
Q

heavy chain protein

A

lymphoid neoplasms - produce fc portion

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19
Q

precipitation reaction

A 
blood
semen
adulteration in food
streptococcus grouping
vdrl syphilis
standardisation
diphtheria toxigenicity
20
Q

precipitation tests

A

ring test
slide test
tube test
immunodiffusion

21
Q

ring test ppt

A

thermoprecipitin ascoli’s

lancefield strept group

22
Q

slide test ppt

A

vdrl syphilis

23
Q

tube test ppt

A

kahn test for syphilis

standardized toxins

24
Q

LF (lime flocculation) dose of toxoid

A

amount of toxin that forms floccules with one unit of antitoxin

25
Q

elek’s test

A

diphtheria toxigenicity

26
Q

counterimmunoelectrophoresis

A

for alpha fefo protein

cryptococcus and meningococcus in csf

27
Q

agglutination

A

routine ag detection

blood grouping

28
Q

agglutination types

A

tube agglutination
heterophile - weil felix and strept MG, Paul bunnell test
coombs test
passive agglutination - rose waaler, latex agglutination, co agglutination

29
Q

tube agglutination

A

typhoid
typhus
brucellosis

30
Q

weil felix agglutination test

A

typhus

rickettsia and proteus strqins share common qg so proteus antigen can be used to detect rickettsia anitbodies

31
Q

strept MG agglutination test

A

primary atypical pneumonia

32
Q

cold agglutination test

A

primary atypical pneumonia

antibodies to human o group rbc - agglutination at 4 degree Celsius

33
Q

paul bunnell agglutination test

A

sheep erythrocytes antibodies
ox red cells (not guinea pig kidneys)

infectious mononucleosis

34
Q

Coombs test

A

direct - in vivo

indirect - in vitro

35
Q

coombs test

A

brucellosis

rh incompatibility

36
Q

carrier particles in passive agglutination

A

latex, rbc, bentonite

37
Q

rose waaler test - passive agglutination

A

rheumatoid arthritis

sheep rbc + sheep rbc antibodies (rabbit) (non agglutinatable)
+ RA FACTOR

38
Q

latex agglutination

A

ASO, CRP, RA FACTOR, HCG

39
Q

coagglutination

A

staph protein A bunds to antibodies that agglutinate other bacteria (legionella)

39
Q

coagglutination

A

staph protein A bunds to antibodies that agglutinate other bacteria (legionella)

40
Q

coagglutination

A

staph protein A bunds to antibodies that agglutinate other bacteria (legionella)

40
Q

coagglutination

A

staph protein A bunds to antibodies that agglutinate other bacteria (legionella)

41
Q

coagglutination

A

staph protein A bunds to antibodies that agglutinate other bacteria (legionella)

41
Q

coagglutination

A

staph protein A bunds to antibodies that agglutinate other bacteria (legionella)

41
Q

coagglutination

A

staph protein A bunds to antibodies that agglutinate other bacteria (legionella)

42
Q

Wasserman reaction cft

A

serodiagnosis of syphilis

Microbiology (7 decks)

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