Antibiotic Resistant and Diagnostic Techniques Flashcards

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1
Q

what is the definition of antibiotics?

A

-drugs designed to kill bacteria

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2
Q

what are the 2 ways that antibiotics usually kill bacteria?

A

-disrupt cell wall formation by stopping the enzyme that is involved in this process, causes cell lysis as the bacterial cell cannot resist the change is osmotic pressure
-inhibit metabolic processes like protein synthesis

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3
Q

what does antibiotic resistance mean?

A

-when bacteria are resistant to one or more antibiotics and are no longer affected by them

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4
Q

what occurs in the bacterial cells which makes antibiotics no longer effective to them?

A

-mutations in the bacterial genome leads to metabolic changes which results in antibiotics being no longer effective

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5
Q

why can a large population of bacteria become antibiotic resistant very quickly?

A

-bacteria produce very rapidly by binary fission which means if one bacterium is resistant a large population become resistant very quickly

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6
Q

what does the term antiobiotic winter mean?

A

-some antibiotic resistant bacteria are resistant to many types of antibiotics which poses the threat that some could become resistant to all antibiotics

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7
Q

the number of deaths in recent years from MRSA has decreased (bacteria that is resistant to a lot of antibiotics) why is this?

A

-not due to the amount of antibiotic resistance decreasing but it is due to better hygiene practice in hospitals, more effective isolation and new targeted drug programmes

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8
Q

what are the benefits to society and economy of discovering new antibiotics?

A

-more effective treatment
-less treatment costs
-less time off work

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9
Q

where is one of the new places that scientists are investigating for new antibiotics?

A

-in the soil
-where some types of microbes naturally produce antimicrobial substances

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10
Q

what are some of the factors that affect how quickly a disease spreads?

A

-how easily it is spread
-how likely someone will fall ill to it if infected
-whether there is a vaccination for that disease
-whether bacterial resistance has occurred

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11
Q

what is the definition of an epidemic?

A

-a disease that spreads through a small region usually one country which affects a high amount of the population

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12
Q

what is the definition of a pandemic?

A

-diseases affecting many thousands of people or several countries at the same time

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13
Q

what are most major epidemics and pandemics caused by?

A

-viruses

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14
Q

what are the 3 reasons why viruses are more likely to cause epidemics and pandemics?

A

-they are very small and prone to mutation
-many viruses are known as retroviruses with RNA which makes them less stable
-antibiotics are not effective against viruses

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15
Q

what are reservoir species?

A

-animal species that harbour viruses that subsequently cause diseases in humans

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16
Q

what are some characteristics about a reservoir species?

A

-they suffer little harm from the virus
-not adapted to transfer the pathogen
-if transfer occurs it us usually by chance

17
Q

what animal is most common as a reservoir species and why?

A

-bats
-similar physiology to humans
-social animals with other bats making the possibility of cross infection likely
-fly large ranges so greater number will be infected

18
Q

what is the most common factor which contributes to the spread of infection from bats to humans?

A

-urbanistaion and clearing of woodlands

19
Q

what can earlier and more accurate diagnosis lead to?

A

-more effective treatment

20
Q

what are the two things that have helped the advances in diagnosing in recent years?

A

-use of ELISA techniques
-detection of cytokines as biomarkers

21
Q

what are cytokines?

A

-molecules that are involved in signaling between different types of immune cells
-an increase in cytokines show an increase in immune responses

22
Q

what does ELISA stand for?

A

-Enzyme-linked immunosorbent assay

23
Q

what does the ELISA techniques involve?

A

-uses antibodies, enzymes and other molecules as biomarkers to detect the presence of particular molecules in the body
-these tests can screen for a large number of antigens at the same time for a number of possible conditions

24
Q

what do ELISA tests use from the individual? what happens to it?

A

-body fluids are added to a sophisticated spotting tray and a range of antibodies are added
-a reaction between antibody and antigen triggers an enzyme which causes a colour change which identifies the antibody/ antigen present

25
Q

what are the 4 things that ELISA kits can be used to detect?

A

-pathogens in the body
-cancer cell markers
-cardiac disease markers
-pregnancy in at home kits

26
Q

what type of feedback do ELISA kits provide?

A

-a wide range of feedback from one test

27
Q

what are two examples of these wide range of diagnostic feedbacks that can occur from one test?

A

-pregnancy testing- following egg implantation the levels of hCG hormone increase it is this increase that is detected in the ELISA plate
-testing for viral pathogens- the ELISA plate in impregnated with viral antigens which is then coated with blood serum from the individual, if the patient has complementary antibodies are triggered which causes an enzyme reaction which leads to a colour change