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Chem 23 > Analytical Instrumentation > Flashcards

Analytical Instrumentation Flashcards

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1
Q

works like a prism and separates the light into its component wavelengths

A

diffraction grating

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2
Q

the _____________ measures the transmittance and absorbance of the sample

A

detector

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3
Q

the detector measures the _________________ and ____________ of the sample

A

transmittance, absorbance

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4
Q

It refers to the amount of light that passes completely through the sample and strikes the detector

A

Transmittance

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5
Q

It is a measurement of light that is absorbed by the sample

A

Absorbance

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6
Q

The detector senses the light being transmitted through the sample and converts this information into a _____________________

A

digital display

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6
Q

It is the study of how light interact with matter

A

Spectroscopy

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7
Q

This works because lights gets absorbed by matter

A

Spectrophotometry

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8
Q

higher concentration = light absorbed?

A

more light gets absorbed

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9
Q

higher concentration = light transmitted?

A

less lights gets transmitted

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10
Q

Components of spectrophotometry:

A

light source
collimator (lens)
monochromator (prism)
wavelength selector
sample dilution
detector
digital display

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11
Q

This measures the incoming light and the result gets displayed

A

Detector

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12
Q

This value is known as the absorbance value which is also often called?

A

optical density or od

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13
Q

This value that gets displayed is known as the?

A

absorbance value

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14
Q

It shows us how much light and at which wavelength it gets absorbed by our sample

A

Absorption spectrum

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15
Q

In the absorption spectrum, the y-axis is the?

A

absorbance

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16
Q

In the absorption spectrum, the x-axis is the?

A

wavelength

17
Q

To calculate the specific concentration of the solution used in the spectrometer, we use?

A

Beer’s law

18
Q

This can be used to quantify the amount or a substance in a solution

A

Spectrophotometry

19
Q

It is especially useful when we want to measure the change in concentration of a solution over time

A

Spectrophotometry

20
Q

It is widely used in chemistry, physics, biochemistry, and materials and chemical engineering

A

Spectrophotometry

21
Q

It is typically used to measure wavelengths of electromagnetic radiation (light) that have interacted with a sample

A

spectrometer

22
Q

It is a chromatography technique in which separation is carried out in column

A

Column Chromatography

23
Q

The column is filled with the stationary phase which can either be?

A

silica
alumina
cellulose

24
Q

The bottom of the column is packed with ______________ to support the stationary phase

A

cotton or glass wool

25
Q

The column preparation is usually carried out by two methods:

A

wet method
dry method

26
Q

In this method, the stationary phase is mixed with mobile phase to make a slurry and then packed in the column

A

wet method

27
Q

In this method, the powder of the stationary phase is directly added to the column.

A

dry method

28
Q

mobile phase = ?

A

eluent

29
Q

The mobile phase leaving the column is known as

A

eluate

30
Q

The sample leaving the column is known as

A

eluite

31
Q

Other forms of column chromatography:

A

adsorption chromatography
Ion exchange chromatography
affinity chromatography
gel permeation chromatography

32
Q

If stationary phase is adsorbent material like silica and alumina, then its known as

A

adsorption chromatography

33
Q

Is stationary phase is ion exchange resin, then its known as I

A

on exchange chromatography

34
Q

If the stationary phase is attached with the ligand that binds specific molecules such as protein or enzyme, then it is called as

A

affinity chromatography

35
Q

If the stationary phase is a porous gel or a bead, then its known as

A

gel permeation chromatography

36
Q

It is based on adsorption chromatography

A

thin layer chromatography

37
Q

Adsorbent (silica, alumina) is coated on?

A

glass slide
plastic surface
inert solid surface

38
Q

Thickness of adsorbent material = ?

A

0.1 mm to 0.3 mm

39
Q

Once adsorbent material is coated, the plates are kept in?

A

oven

40
Q

Coloured samples can be visualized directly

A

thin layer chromatography

41
Q

It is usually used to detect analytes that gives fluorescence

A

UV light

Chem 23 (7 decks)

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