Analysis Of Cell Components

Question 1

What is the primary purpose of using microscopes in biology?

Answer 1

To magnify and visualize small cell components that are not visible to the naked eye.

Question 2

True or False: An optical microscope uses light to illuminate the specimen.

Answer 2

True

Question 3

Fill in the blank: The __________ microscope provides a higher resolution than an optical microscope.

Answer 3

electron

Question 4

What is the maximum magnification typically achievable with an optical microscope?

Answer 4

Around 1000x to 2000x.

Question 5

Which type of microscope would you use to observe the surface structure of a cell?

Answer 5

Scanning Electron Microscope (SEM).

Question 6

What is the function of the objective lens in a microscope?

Answer 6

To collect light from the specimen and form a magnified image.

Question 7

True or False: Electron microscopes can only be used to view live specimens.

Answer 7

False

Question 8

What is the role of the diaphragm in a light microscope?

Answer 8

To control the amount of light that reaches the specimen.

Question 9

What type of microscopy uses fluorescent dyes to highlight specific components?

Answer 9

Fluorescence microscopy.

Question 10

What is the advantage of using a Transmission Electron Microscope (TEM)?

Answer 10

It provides detailed internal structure images of cells.

Question 11

What is the purpose of staining in microscopy?

Answer 11

To enhance contrast in the specimen for better visibility.

Question 12

Multiple Choice: Which of the following is NOT a type of electron microscope? A) TEM B) SEM C) LM D) STEM

Answer 12

C) LM

Question 13

What is the resolution limit of a typical light microscope?

Answer 13

About 200 nanometers.

Question 14

Fill in the blank: The __________ is the part of the microscope that holds the objective lenses.

Answer 14

revolving nosepiece

Question 15

True or False: Live cell imaging can be performed using electron microscopy.

Answer 15

False

Question 16

What type of sample preparation is required for electron microscopy?

Answer 16

Samples must be dehydrated and often coated with a conductive material.

Question 17

What is the function of the ocular lens in a microscope?

Answer 17

To further magnify the image formed by the objective lens.

Question 18

What is a common use of a phase contrast microscope?

Answer 18

To observe living cells without staining.

Question 19

Fill in the blank: The __________ of a microscope helps to adjust the focus.

Answer 19

coarse and fine focus knobs

Question 20

What kind of specimen can be viewed with a dark field microscope?

Answer 20

Thin or transparent specimens that are hard to see with standard light.

Question 21

Multiple Choice: Which of the following microscopy techniques provides the best resolution? A) Light microscopy B) Scanning Electron Microscopy C) Transmission Electron Microscopy D) Phase Contrast Microscopy

Answer 21

C) Transmission Electron Microscopy

Question 22

What is the primary difference between a SEM and a TEM?

Answer 22

SEM provides 3D images of surface structures, while TEM provides 2D images of internal structures.

Question 23

True or False: The field of view is larger in an optical microscope compared to an electron microscope.

Answer 23

True

Question 24

What is the advantage of using a confocal microscope?

Answer 24

It allows for the collection of sharp images at various depths within a specimen.

Question 25

Fill in the blank: The __________ microscope is particularly useful for viewing the movement of live cells.

Answer 25

live-cell imaging