ANACHEM LAB Flashcards
is a form of energy that propagates as both electrical and magnetic waves travelling in packets of energy called photons
Electromagnetic Radiation (EMR)
Wavelength of the Color Violet
400-450nm
Wavelength of the Color Blue
450-495nm
Wavelength of the Color Cyan
495-520nm
Wavelength of the Color Green
520-565nm
Wavelength of the Color Yellow
565-590nm
Wavelength of the Color Orange
590-620nm
Wavelength of the Color Red
620-700nm
Wavelength of the Ultraviolet Light
1-400nm
Wavelength of the Infrared Light
2.5nm- 25nm
Wavelength of Microwave
25um to 1mm
Wavelength of Radiowaves
> 1mm
Wavelength of X rays
1nm- 1pm
Wavelength of Gamma Rays
<10^-12m
Describes the relationship between Absorbance of light and the properties of their medium through which the light is travelling.
Beer- Lambert Law
Formula for Beer Lamber Law
A= ECl
A= Absorbance
E= Molar Absorptivity
l= Path Length
C= Concentration
shorter wavelength have ________________ energy while longer wavelength will have _____________________
Higher, Lower
Photometry that uses the principle of atoms when in a high energy state emit light when they are returned to a lower energy state. The emitted light is what is used to determine the concentration of specific elements in a sample
ATOMIC EMISSION SPECTROPHOTOMETRY
Photometry that measure the absorption of light by atoms in a lower energy state.
ATOMIC ABSORPTION SPECTROPHOTOMETRY
Photometry that revolves around the emission of light by molecules that have absorbed energy (Fluorescence and Phosphorescence)
MOLECULAR LUMINESCENCE SPECTROSCOPY
Measures the intensity of scattered light caused by particles in a suspension
Nephelometry
measures the reduction in light intensity as it passes through a turbid solution
Turbidity
is based on the bending of light as it passes through a substance
Refractometry
Measures the osmotic pressure of a solution
Osmometry
Measures the Hydrogen Ion Concentration in a solution, indicates its acidity or alkalinity
pH Electrode
Measures the partial pressure of Carbon Dioxide in a solution or Gas
pCO2 Electrode
Selectively measures the concentration of specific ions by detecting changes in voltage
Ion-selective Electrode
separates charged particles, typically proteins or nucleic acid in a gel or other medium using an electric field
ELECTROPHORESIS
Separates Components in a mixture based on their movement through a stationary phase under the influence of a mobile phase
Chromatography
separates volatile compounds based on their retention times in a gas phase
Gas Chromatography
Identifies and quantifies volatile compounds based on their mass-to-charge ratio
Mass Spectrometry
Principles Included in Photometry
- Atomic Emission Photometry
- Atomic Absorption Photometry
- Molecular Luminescence Spectroscopy
Principles included in Non-absorptive Methods
- Nephelometry
- Turbidimetry
- Refractometry
- Osmometry
Principles included in Electrochemistry
- pH Electrode
- pCO2 Electrode
- Ion-selective Electrode