AMYLASE Flashcards
sytematic name of amylase
EZ3211
AMY CATALIZES THE BREAKDOWN OF THE _ AND _
STARCH AND GLYCOGEN
STARCH IS MADE OF _ AND _
AMYLOSE AND AMYLOPECTIN
AMYLOSE IS _ WHILE AMYLOPECTIN IS _
AMYLOSE HAS UNBRANCHED CHAIN AND AMYLOPECTIN HAS BRANCHED CHAIN
AMYLOPECTIN IS A POLYSASCHARIDE WITH A _
a-1-6 LINKAGE
AMYLOSE IS A GLUCOSE LINKED BY _
a-1-4 GLYCSIDIC BONDS
WHAT IS THE SOURCE OF ENERGY FOR AMYLASE ?
GLUCOSE
ALPHA AMY ATTACKS ONLY THE _ TO PRODUCE DEGRADATION PRODUCTS
a-1-4 GLOSIDIC BONDS
DEGRADATION PRODUCTS OF AMY
GLUCOSE, MALTOSE, AND INTERMIDIATE CHAINS(DEXTRINS)
MW OF AMY
50,000-55,000
_ IS THE MOST IMPORTANT ENZYME IN THE DIGESTION OF STARCHES AND IT REQUIRES _ AND _ FOR ACTIVATION
AMYLASE, CALCIUM AND CHLORIDE IONS
THIS ISOENZYME OF AMY IS FOUND IN THE SALIVARY GLANDS, FALLOPIAN TUBE AND LUNG
S-TYPE ISOAMYLASE (PYTYALIN)
IN WHAT TISSUES ARE AMY FOUND IN
PANCREAS AND SALIVARY GLAND
P-TYPEN ISOAMYLASE( AMYLOPSIN) : WHERE IS IT DERIVED FROM AND WHAT INHIBITS THE ISO
DEREIVED FROM PANCREATIC TISSUE AND INHIBITED BY MONOCLOCNAL ANTIBODY ONLY
TRUE OR FALSE: PTYALIN CAN BE INHIBITED BY MONOCLONAL ANTIBODY ONLY
FALSE PTYALIN ( S TYPE) CAN ALSO BE INHIBITED BY PROTEIN FROM WHEAT
ISOENZYMES THAT MIGRATES MOST QUICKLY:
S1,S2,S3
ACUTE PANCREATITIS(AMS )
P3 PREDOMINATES
MOST COMMONLY OBSERVED FRACTIONS OF AMY ISOENZYMES :
S1,S2 AND P2
_OF STYPE AMS ACT. OF NORMAL SERUM
2/3 AMS ACT.
P-TYPE PREDOMINATES IN _
NORMAL URINE
ELEVATED AMY = _
ACUTE PANCREATITIS
ABNORMAL AMY IN COMBINATION W/ IGG WITH HIGH MW
MACROAMYLASE
MACRO AMY IS NOT FOUND IN _ AND CONC. IN _
URINE AND CONC. IN SERUM/PLASMA
MACRO AMY SHOULD BE DIFFERENTIATED FROM OTHER CAUSES OF _
HYPERAMYLESIA
AMS ACT ACCORDING TO HENRY:
RISE: 2-12 HRS PEAK @ 48 HRS ,< IN THE 3/5 DAY
AMS ACT ACCORDING TO TIETZ
RISE 5-8 HRS, PEAK 12-72 HRS , , IN 3/4 DAY
METHOD FOR AMS THAT IS MORE CONTROLLED AND CONSISTENT HYDROLYSIS CONDITIONS AND USED TO MONITOR AMS ACT.
CONTINOUS MONITORING ASSAY
LIST THE HISTORICAL METHODS FOR AMS
SACCHAROGENIC, AMYLOCLASTIC, CHROMOGENIIC
DEFINE SOMOGYI UNITS
NUMBER OF MM OF GLUCOSE IN 30 MIN. @37C
THIS IS A IODOMETRIC METHOD AND THE HYDROLYSIS OF STARCH >DEC. IN COLOR INTENSITY
AMYLOCLASTIC METHOD
CONVERSION FACTOR FOR IU AND SOMOGYI:
1.85
REFERENCE METHOD FOR AMS AND USES SOMOGYI UNIT
SACCHAROGENIC( NELSON SOMGYI MOD. BY HENRY AND CHIAMON)
IN THE NAME OF KLEIN, FOREMAN AND SEARCY, IT USES STARCH ATTACHED WITH DYE
CHROMOGENIC METHOD
COLOR OF CHROMOGENIC METHOD
BLUE LIKE THE OCEAN
CHROMOGENIC METHOD MEASURES THE _ IN COLOR INTENSITY
INCREASE
THIS METHOD MEASURESS THE TURBIDITY OF THE SAMPLE
TURBIDITY AND NEPHELOMETRY
THIS OPIATES BLOOD SAMPLES>FALSE DEC IN AMS LEVELS
MORPHINE ( MAY KANTA YATA SI ARAINA GRANDE ABOUT THIS)
SALIVARY AMY IS INHIBITED BY_
WHEAT GERM LECTIN
NORMAL SERUM HAS :
1 TO 4 % (O.O1-O.O4)
A:C RATIO :
> 4% UP YO 15 %
