ALP Flashcards
transcribe alt and give its systematic name
ALKALINE PHOSPHATASE , EC 3131
PH OH ALP AND ITS ACTIVATOR
9-10 PH, MAGNESIUM
PRODUCT OF ALP
INROGANIC PHOSPHATE ION
LIST THE MAJOR ISOENZYMES OF ALP
LIVER ,BONE, PLACENTA AND INTESTINAL
HIGHEST ELEVATION:
HEPATOBILLIARY DISORDERS AND BONE DISORDERS
BILLIARY TRACT OBSTRUCTION
CAUSED BY THE SYNTHESIS OF THE ENZYME INDUCED BY CHLESTASIS, 3-10X ULN
HEPATOCELLULAR DISORDERS
< 3 ULN
SEEN IN PAGETS DX, OSTEOmalacia, ETC…. masakit na fingers ko
BONE ISOENZYME
SEEN IN DM, RENAL FAILURE AND CIRRHOSIS, SEN IN B T O AND B
INTESTINAL ISOENZYME
elevated after MEALS
INTESTINAL ISOENZYMES
SEEN IN PREGNANT WOMEN
PLACENTAL ISOENZYMES
NORMAL ULN OF PLACENTAL ISO IN WOMEN
1 1/2 ULN
ELEVATIONS MAY ALSO BE SEEN IN COMPLICATIONS OF PREGNANCY TRUE OR FALSE?
TRUE *BASTA MAY “cLAMPSIA”
ALP IS DECREASED IN _ AND _
HYPOPHOSPHATASIA AND ABSENCE OF BONE ISO
ALP ISO IN CANCER ALSO KNOWN AS :
CARCINO PLACENTAL ALKALINE PHOSPHATASE
MOST HEAT STABLE CANCER ALP ISO
REGAN ISOENZYME
ECTOPIC PRODUCTION AND SEEN IN LUNG BREAST COLON,GYNECOLIGIC CA
REGAN SYEMPRE
REGAN MIGRATES TO THE SAME POSITION AS THE _
HYPOPHOSPHATASIA AND ABSENCE OF BONE ISO
REGAN RESISTS HEAT AT _ FOR _
65C FOR 30 MIN
REGAN IS INHIBITED BY _
PHENYLALANINE
A VARIANT OF REGAN
NAGAO ISO
NAGAO IS FOUND IN
ADENOCARCINOMA OF THE PANCREAS
NAGAO IS INHIBITED BY
L-LEUCINE NAD PHENYLALANINE
CANCER ISO THAT IS FOUND IN HEPATOMA
KASAHARA
ASSAY FOR ALP THAT USES PARANITROPHENYLPHOSOHATE SUBSTRATE
BOWERS AND MCCOMB (SZASZ MOD.)
B AND M’S ASSAY RX IS BASED ON:
MOLAR ABSORPITVITY OF PARA NITROPHENOL
_ FALSELY LOWER ACT. OF B AND M ASSAY
CHELATORS
MOST SPECIFIC METHOD FOR ALP AND STABLE AT 4C UP TO A WEEK
BOWERS AND MCCOMB (SZASZ MOD.)
B AND M MEASURES ALP AT _ MM AT _ PH
405 MM AT 10.2 PH
A LIVER FRACTION OF LIVER ISO THAT IS INCREASED IN HEPATOBILLIARY CONDITIONS
MAJOR LIVE BAND
SUBSTRATE : BETA GLYCEROL PHOSPHATE > PRODUCT: INORGANIC PHOSPHATE GLYCEROL
METHODS: BODANSKY, SHINOWARA, JONES, REINHART
FRACTION OF THE OF LIVER ISO THAT INCREASES IN METATASTIC CARCINOMA AND INDICATOR OF OBSTRUCTIVE LIVER DISEASE
FAST LIVER (a-1)
SUBSTRATE: PHENYLPHOSPHATE PHENOL > PRODUCT : PHENOL
METHODS: BODANSKY, SHINOWARA, JONES, REINHART
SUBSTRATE : PARA NITROPHENYL PHOSPHATE > PRODUCT: PARA NITROPHENOL/ YELLOW NITROPHENOXIDE ION
METHODS: BESSY LOWRY AND BLOCK, BOWERS AND MCCOMB
SUBSTRATE: PHENOLPHTHALEIN DIPHOSPHATE> PRODUCT : PHENOLPHTHALEIN RED
METHOD : HUGGINS AND TALALAY
SUBSTRATE AND PRODUCT : ALPHANAPHTHOL PHOSPHATE > ALPHA NAPHTHOL
MOSS
BUFFERED PHENOLNAPHTHALEIN > FREE PHENOLPHTHALEIN
BY KLEIN BABSON AND READ
IN CASE OF HEMOLYSIS :
ALP IS 6X MORE CONC. IN ERYTHROCYTES THAN IN SERUM
ACTICUTY IN SERUM INC. -% ON STANDING AT 25C/_C FOR SEVERAL HRS.
3-10%, 25C/4C
DIET _ ALP ACTIVITY OF BLOOD GROUP_ AND _ WHO ARE SECRETORS
ELEVATES ALP , BLOOD GROUP B AND O
TRUE/FALSE : ZINC IS A COMPONENT OF ALP
TRUE *BASTA MAY “cLAMPSIA”
_ALP THAT IS A USEFUL TUMOR MARKER IN SERUM AND CSF
PLACENTAL ALP
