ALP Flashcards

1
Q

transcribe alt and give its systematic name

A

ALKALINE PHOSPHATASE , EC 3131

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2
Q

PH OH ALP AND ITS ACTIVATOR

A

9-10 PH, MAGNESIUM

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3
Q

PRODUCT OF ALP

A

INROGANIC PHOSPHATE ION

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4
Q

LIST THE MAJOR ISOENZYMES OF ALP

A

LIVER ,BONE, PLACENTA AND INTESTINAL

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5
Q

HIGHEST ELEVATION:

A

HEPATOBILLIARY DISORDERS AND BONE DISORDERS

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6
Q

BILLIARY TRACT OBSTRUCTION

A

CAUSED BY THE SYNTHESIS OF THE ENZYME INDUCED BY CHLESTASIS, 3-10X ULN

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7
Q

HEPATOCELLULAR DISORDERS

A

< 3 ULN

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8
Q

SEEN IN PAGETS DX, OSTEOmalacia, ETC…. masakit na fingers ko

A

BONE ISOENZYME

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9
Q

SEEN IN DM, RENAL FAILURE AND CIRRHOSIS, SEN IN B T O AND B

A

INTESTINAL ISOENZYME

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10
Q

elevated after MEALS

A

INTESTINAL ISOENZYMES

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11
Q

SEEN IN PREGNANT WOMEN

A

PLACENTAL ISOENZYMES

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12
Q

NORMAL ULN OF PLACENTAL ISO IN WOMEN

A

1 1/2 ULN

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12
Q

ELEVATIONS MAY ALSO BE SEEN IN COMPLICATIONS OF PREGNANCY TRUE OR FALSE?

A

TRUE *BASTA MAY “cLAMPSIA”

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13
Q

ALP IS DECREASED IN _ AND _

A

HYPOPHOSPHATASIA AND ABSENCE OF BONE ISO

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13
Q

ALP ISO IN CANCER ALSO KNOWN AS :

A

CARCINO PLACENTAL ALKALINE PHOSPHATASE

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14
Q

MOST HEAT STABLE CANCER ALP ISO

A

REGAN ISOENZYME

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15
Q

ECTOPIC PRODUCTION AND SEEN IN LUNG BREAST COLON,GYNECOLIGIC CA

A

REGAN SYEMPRE

16
Q

REGAN MIGRATES TO THE SAME POSITION AS THE _

A

HYPOPHOSPHATASIA AND ABSENCE OF BONE ISO

17
Q

REGAN RESISTS HEAT AT _ FOR _

A

65C FOR 30 MIN

18
Q

REGAN IS INHIBITED BY _

A

PHENYLALANINE

19
Q

A VARIANT OF REGAN

A

NAGAO ISO

20
Q

NAGAO IS FOUND IN

A

ADENOCARCINOMA OF THE PANCREAS

21
Q

NAGAO IS INHIBITED BY

A

L-LEUCINE NAD PHENYLALANINE

22
Q

CANCER ISO THAT IS FOUND IN HEPATOMA

A

KASAHARA

23
Q

ASSAY FOR ALP THAT USES PARANITROPHENYLPHOSOHATE SUBSTRATE

A

BOWERS AND MCCOMB (SZASZ MOD.)

24
Q

B AND M’S ASSAY RX IS BASED ON:

A

MOLAR ABSORPITVITY OF PARA NITROPHENOL

25
Q

_ FALSELY LOWER ACT. OF B AND M ASSAY

A

CHELATORS

26
Q

MOST SPECIFIC METHOD FOR ALP AND STABLE AT 4C UP TO A WEEK

A

BOWERS AND MCCOMB (SZASZ MOD.)

27
Q

B AND M MEASURES ALP AT _ MM AT _ PH

A

405 MM AT 10.2 PH

28
Q

A LIVER FRACTION OF LIVER ISO THAT IS INCREASED IN HEPATOBILLIARY CONDITIONS

A

MAJOR LIVE BAND

29
Q

SUBSTRATE : BETA GLYCEROL PHOSPHATE > PRODUCT: INORGANIC PHOSPHATE GLYCEROL

A

METHODS: BODANSKY, SHINOWARA, JONES, REINHART

30
Q

FRACTION OF THE OF LIVER ISO THAT INCREASES IN METATASTIC CARCINOMA AND INDICATOR OF OBSTRUCTIVE LIVER DISEASE

A

FAST LIVER (a-1)

31
Q

SUBSTRATE: PHENYLPHOSPHATE PHENOL > PRODUCT : PHENOL

A

METHODS: BODANSKY, SHINOWARA, JONES, REINHART

32
Q

SUBSTRATE : PARA NITROPHENYL PHOSPHATE > PRODUCT: PARA NITROPHENOL/ YELLOW NITROPHENOXIDE ION

A

METHODS: BESSY LOWRY AND BLOCK, BOWERS AND MCCOMB

33
Q

SUBSTRATE: PHENOLPHTHALEIN DIPHOSPHATE> PRODUCT : PHENOLPHTHALEIN RED

A

METHOD : HUGGINS AND TALALAY

34
Q

SUBSTRATE AND PRODUCT : ALPHANAPHTHOL PHOSPHATE > ALPHA NAPHTHOL

A

MOSS

35
Q

BUFFERED PHENOLNAPHTHALEIN > FREE PHENOLPHTHALEIN

A

BY KLEIN BABSON AND READ

36
Q

IN CASE OF HEMOLYSIS :

A

ALP IS 6X MORE CONC. IN ERYTHROCYTES THAN IN SERUM

37
Q

ACTICUTY IN SERUM INC. -% ON STANDING AT 25C/_C FOR SEVERAL HRS.

A

3-10%, 25C/4C

38
Q

DIET _ ALP ACTIVITY OF BLOOD GROUP_ AND _ WHO ARE SECRETORS

A

ELEVATES ALP , BLOOD GROUP B AND O

39
Q

TRUE/FALSE : ZINC IS A COMPONENT OF ALP

A

TRUE *BASTA MAY “cLAMPSIA”

40
Q

_ALP THAT IS A USEFUL TUMOR MARKER IN SERUM AND CSF

A

PLACENTAL ALP