Amoebae, flagellates, cilliates, tissue protozoa Flashcards
What parasite is this?
Chilomastix mensili
What is this parasite? and what stage?
Entamoeba coli
TROPHOZOITE
What is this parasite? and what stage?
Entamoeba hystolica (w/ RBC’s)
Entamoeba dispar
Entamoeba moshkovski
Entamoeba bangladeshi
TROPHOZOITE STAGE
What parasite is this? and what stage?
Endolimax nana
Cyrptosporidium hominis and Cryptosporidium parvum
route of infection fecal-oral route, Auto-infection
geography: waterborne
Lab diagnosis
stool sample – modified acid-fast stain, immunoassay, direct and indirect fluorescent antibody tests
Morphology
trophozoites - rarely seen
cysts - oocysts are generally round, 4-6 micrometers, each mature oocyst contains sporozoites
Pathology
asymptomatic, immunocompetent - self limiting
symptomatic- watery diarrhea, dehydration
chronic infection in immunocompromised: extra-intestinal infection: respiratory infection, death
Chilomastix mensili
Life cycle: ingested eggs contaminated water or fruit
Lab diagnosis
specimen source - stool sample, Lab test: ova and parasite permanent stain
Pathology – none (nonpathogen/commensal)
Giardia lambia
Life cycle: ingested ova in contaminated drinking water
Lab diagnosis
nspecimen source - stool sample
ova and parasite exam - , concentrate and permanent stain.
nparasite morphology
nother lab tests – EIA/ELISA- Giardia antigen test, duodenal aspirate, Entero-Test®, direct fluorescent antibody (DFA) test
Pathology – diarrhea, ? Eosinophilia
Cystoisopora belli
Life cycle: ingestion mature oocysts w/ sporozoites
Lab diagnosis
specimen source - stool sample
morphology–oocyst: 20-33 by 10-19 micrometers
Modified acid fast stain
Pathology
Asymptomatic, immunocompetent- self limiting
symptomatic , immunocompromised patient- anorexia, nausea, abdominal pain, diarrhea, possibly malabsorption, eosinophilia
What parasite is this?
Dientamoeba fragilis
What is this parasite? and what stage?
Entamoeba hystolitica
Entamoeba dispar
Entamoeba moshkovski
Entamoeba bangladeshi
CYST STAGE
Toxoplasma gondii
life cycle: ingestion of cysts from cat litter, animals, or food/water
specimen source for antibody (ab) - serum sample- serology; immunology test, done on children and adults
specimen source for active form: tachyzoites/ trophozoites, crescent shape 4-8 mcm (um) in length –CSF – Wright-Giemsa stain, bronchoalveolar lavage fluid, amniotic fluid- Molecular/PCR techniques: done on immunocompromised, newborn; pregnant females- amniotic fluid
Pathology
- children, adults – Infectious mononucleosis like syndrome, child affected as a fetus will have problems listed below:stillborn, mental disabilities, blindness
- fetus - stillborn, mental disabilities, blindness
- immunocompromised – Central Nervous System disease
What parasite is this?
Iodomoeba butschlii
Sarcocystis spp.
Life cycle - two host life cycle
route of infection
- Contaminated meat – ingest sarcocyst and it goes to oocyst in our GI tract, we are adult host
- Contaminated food or water- ingest sporocyst and it goes to a sarcocyst in our tissue, we are intermediate host
Lab diagnosis
specimen source - stool sample
morphology – sporocyst – 9-16 micrometers, lab stain- modified acid fast stain,
Pathology – immunocompromised patient
fever, diarrhea, abdominal pain
what parasite is this
Balantidium coli
Entamoeba histolytica
Life cycle : fecal-oral route, contaminated water Body location: intestine Lab diagnosis: o+p exam, concentrate, permanent stains, ELISA, PCR, morphology ENGULFED RBC’s! Pathology: asymptomatic, dysentery, invasion leads to organ failure, death.