Amoebae, flagellates, cilliates, tissue protozoa Flashcards

1
Q

What parasite is this?

A

Chilomastix mensili

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2
Q

What is this parasite? and what stage?

A

Entamoeba coli

TROPHOZOITE

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3
Q

What is this parasite? and what stage?

A

Entamoeba hystolica (w/ RBC’s)

Entamoeba dispar

Entamoeba moshkovski

Entamoeba bangladeshi

TROPHOZOITE STAGE

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4
Q

What parasite is this? and what stage?

A

Endolimax nana

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5
Q

Cyrptosporidium hominis and Cryptosporidium parvum

A

route of infection fecal-oral route, Auto-infection

geography: waterborne

Lab diagnosis

stool sample – modified acid-fast stain, immunoassay, direct and indirect fluorescent antibody tests

Morphology

trophozoites - rarely seen

cysts - oocysts are generally round, 4-6 micrometers, each mature oocyst contains sporozoites

Pathology

asymptomatic, immunocompetent - self limiting

symptomatic- watery diarrhea, dehydration

chronic infection in immunocompromised: extra-intestinal infection: respiratory infection, death

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6
Q

Chilomastix mensili

A

Life cycle: ingested eggs contaminated water or fruit

Lab diagnosis

specimen source - stool sample, Lab test: ova and parasite permanent stain

Pathology – none (nonpathogen/commensal)

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7
Q

Giardia lambia

A

Life cycle: ingested ova in contaminated drinking water

Lab diagnosis

nspecimen source - stool sample

ova and parasite exam - , concentrate and permanent stain.

nparasite morphology

nother lab tests – EIA/ELISA- Giardia antigen test, duodenal aspirate, Entero-Test®, direct fluorescent antibody (DFA) test

Pathology – diarrhea, ? Eosinophilia

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8
Q

Cystoisopora belli

A

Life cycle: ingestion mature oocysts w/ sporozoites

Lab diagnosis

specimen source - stool sample

morphology–oocyst: 20-33 by 10-19 micrometers

Modified acid fast stain

Pathology

Asymptomatic, immunocompetent- self limiting

symptomatic , immunocompromised patient- anorexia, nausea, abdominal pain, diarrhea, possibly malabsorption, eosinophilia

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9
Q

What parasite is this?

A

Dientamoeba fragilis

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10
Q

What is this parasite? and what stage?

A

Entamoeba hystolitica

Entamoeba dispar

Entamoeba moshkovski

Entamoeba bangladeshi

CYST STAGE

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11
Q

Toxoplasma gondii

A

life cycle: ingestion of cysts from cat litter, animals, or food/water

specimen source for antibody (ab) - serum sample- serology; immunology test, done on children and adults

specimen source for active form: tachyzoites/ trophozoites, crescent shape 4-8 mcm (um) in length –CSF – Wright-Giemsa stain, bronchoalveolar lavage fluid, amniotic fluid- Molecular/PCR techniques: done on immunocompromised, newborn; pregnant females- amniotic fluid

Pathology

  • children, adults – Infectious mononucleosis like syndrome, child affected as a fetus will have problems listed below:stillborn, mental disabilities, blindness
  • fetus - stillborn, mental disabilities, blindness
  • immunocompromised – Central Nervous System disease
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12
Q

What parasite is this?

A

Iodomoeba butschlii

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13
Q

Sarcocystis spp.

A

Life cycle - two host life cycle

route of infection

  • Contaminated meat – ingest sarcocyst and it goes to oocyst in our GI tract, we are adult host
  • Contaminated food or water- ingest sporocyst and it goes to a sarcocyst in our tissue, we are intermediate host

Lab diagnosis

specimen source - stool sample

morphology – sporocyst – 9-16 micrometers, lab stain- modified acid fast stain,

Pathology – immunocompromised patient

fever, diarrhea, abdominal pain

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14
Q

what parasite is this

A

Balantidium coli

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15
Q

Entamoeba histolytica

A

Life cycle : fecal-oral route, contaminated water Body location: intestine Lab diagnosis: o+p exam, concentrate, permanent stains, ELISA, PCR, morphology ENGULFED RBC’s! Pathology: asymptomatic, dysentery, invasion leads to organ failure, death.

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16
Q

What parasite is this? and what stage?

A

Entamoeba (dispar, moshkovskii, bangladeshi, coli)

CYST

17
Q

What parasite is this?

A

Giardia lambia

18
Q

Blastocystis hominis

A

Life cycle(route of infection, body location); geography Lab diagnosis specimen source - stool sample ova and parasite exam: parasite morphology: cyst stage: central body, 2-4 nuclei, usually 5-15 mcm Count the number present/field Pathology - when present in large numbers in the absence of other parasites, bacteria or viruses, it may be the cause of diarrhea, cramps, nausea, fever, vomiting and abdominal pain and require therapy.

19
Q

Microsporidia

A

Life cycle: inhalation or ingestion of spores

Diagnosis: electron microscopy, biopsy

Pathology: Severe diarrhea, weight loss, cachexia (immunocompromised)

20
Q

What parasite is this? and what stage?

A

Entamoeba coli

CYST

21
Q

What parasite is this?

A

Trichomonas vaginalis

22
Q

What parasite is this?

A

Trichomonas hominis

23
Q

Entamoeba dispar, E. moshkovskii, E. bangladeshi

A

Life Cycle: fecal-oral, contaminated water Lab diagnosis specimen source - stool sample ova and parasite exam -direct, concentrate, and permanent stain parasite morphology – same as Entamoeba histolytica, but no RBCs are ever found in trophozoites; differentiate by molecular techniques – eg. PCR Pathology - asymptomatic

24
Q

Trichomonas hominis

A

Life cycle: ingestion of trophs in comtaminated water, fruit

Lab diagnosis

specimen source - stool sample

Lab test: ova and parasite permanent stain

morphology = no cyst stage

Pathology - considered nonpathogen,

25
Q

Trichomonas vaginalis

A

Life cycle: infection via trophs in urogenital tract

specimen source - urine, vaginal swab

lab tests - wet prep, vaginal swab culture media – stain with Giemsa, molecular tests

Pathology

female - itching, burning

male - asymptomatic

26
Q

Entamoeba hartmani

A

Life cycle (route of entry, body location); geography Lab diagnosis specimen source - stool sample Lab test: ova and parasite exam: permanent stain Nonpathogen/commensal morphology Looks like Entamoeba histolytica but smaller, differentiate by size Trophozoite size: 5-12 micrometers* (and no RBCs) Cyst size: 5-10 micrometers* Pathology - none (nonpathogen/commensal)

27
Q

Endolimax nana

A

Life cycle (route of entry, body location); geography Lab diagnosis specimen source - stool sample Lab test: ova and parasite exam: permanent stain Nonpathogen/commensal morphology Looks like Entamoeba histolytica but smaller, differentiate by size Trophozoite size: 5-12 micrometers* (and no RBCs) Cyst size: 5-10 micrometers* Pathology - none (nonpathogen/commensal)

28
Q

Balantidium coli

A

Life cycle, fecal-oral route, ww

Lab diagnosis

specimen source - stool sample

Lab test: ova and parasite permanent stain

Stain – Hematoxylin and Eosin may be better than trichrome or iron hematoxylin , or a wet prep

Pathology

asymptomatic, diarrhea, dysentery, abscesses, extraintestinal rare- bronchioalveolar lavage, urinary tract infection, vaginitis

29
Q

Dientomoeba fragilis

A

Life cycle, route of infection,- possibly via helminth ova

Lab diagnosis

specimen source - stool sample

Lab test: ova and parasite permanent stain

parasite morphology – there is a cyst stage, but rare to find.

WBC changes-and unexplained eosinophilia, and Charcot-Leyden crystals, have been associated with it.

Pathology -Can be seen with diarrhea outbreaks in daycare centers.

30
Q

Iodamoeba butschlii

A

Life cycle ( route of entry, body location); geography Lab diagnosis specimen source - stool sample ova and Parasite exam – permanent stain morphology Pathology- none (Nonpathogen/commensal)

31
Q

Entamoeba coli

A

Life cycle: fecal-oral, contaminated water Lab Diagnosis specimen source - stool sample Lab test: ova and parasite exam: permanent stain morphology Pathology: asymptomatic

32
Q

Naegleria fowleri

A

Life cycle, route of infection - contaminated water via nasal passages

Lab diagnosis

specimen source – Cerebral Spinal Fluid (CSF)

lab tests – CSF stained with Wright stain/ Wright-Giemsa stain, or a wet prep

Growth on media: requires living cells ( bacterial or cell culture); does not grow with > 0.4% NaCl

Pathology/Signs/Symptoms

A change in the sense of smell or taste Fever, Sudden, severe headache. Stiff neck. Sensitivity to light. Nausea and vomiting. Confusion. Loss of balance

Primary amoebic ( amebic) meningitis (PAM

33
Q

Cyclospora cayetanensis

A

Life cycle: ingestion of sporulated oocyst in food/water

Lab diagnosis

specimen source - stool sample

morphology- wet mounts - nonrefractile spheres – 8-10 micrometers

trichrome - ghost cells, modified acid fast – variable stain, O and P not of much help

Pathology

self limiting - explosive, watery diarrhea

immunocompromised host - anorexia, fatigue

34
Q

Acanthamoeba spp.

A

Life cycle eye infection via contact lenses

specimen source - corneal scrapings

CSF stains – similar to Naegleria testing, Tissue stains – Periodic Acid Schiff (PAS), Hematoxylin and Eosin, Calcofluor white

Growth on media: may grow without bacteria; not affected by 0.85% NaCl

morphology-

  • trophozoites- 15-25 mcm(um), large karyosome
  • cysts - double walled, hexagonal cyst, 9-27 micrometers (mcm or um)

Pathology - blindness, chronic form of meningioencephalitis ( granulomatous amebic meningitis (GAM)-immunocompromised

35
Q

Plasmodium

A