Aminoglycoside Antibiotics Flashcards
Aminoglycoside antibiotics core structure made of whaT?
1,3 diaminocyclitol linked to one or more aminoglcoside rings
Core structures are what?
Streptidine
2-deoxystreptamine
Spectinamine
What do aminoglycosides inhibit
Protein biosynthesis
How do amino-glycosides inhibit protein biosynthesis?
Binding to 30S ribosomal subunit to the 16S rRNA forming the A site
What step of translation does amino-glycodies interfere with?
Formation of initiation complex, causes premature termination
What does aminoglycosides form from its interruption of protein synthesis?
Nonsense proteins
Result of formation of nonsense proteins due to aminoglycosdies?
Cell wall function impaired
Result of impaired cell wall function in aminoglycosides?
More aminoglycosides (AMG) gets in and stops protein synthesis altogether
AMG positively charged or negatively?
Positively
What needs to be displaced to get AMG in through outer membrane?
Mg++ and Ca++ ions
Why does Mg and Ca need to be displaced?
They form salt bridges with phosphates of phospholipids
What type of transport process is passage through cytoplasmic membrane?
Active transport
How many bacterial resistance mechanisms have evolved to combat AMG?
At least 3
What are the 3 mechanisms that resist AMG?
1) Metabolism
2) Altered Ribosomes
3) Altered AMG uptake
How does the metabolism resistance of AMG work?
Bacteria inactivate AMG by acetylation, adenylation, and phosphorylation
Are AMGs metabolized by humans or bacteria?
Bacteria
How does altered ribosome resistance work?
16S rRNA binding site via point mutations
Which bacteria has displayed altered ribosomes in the past?
Mycobacterium tuberculosis
How does Altered AMG uptake resistance work?
Emergence is far less than resistance due to metabolism
Dangers of AMG?
Ototoxic and nephrotoxic
Difference between Ototoxicity and nephrotoxicity in AMG?
Ototoxicity is irreversible while nephrotoxicity is reversible
Risk factors for ototoxicity?
Using other ototoxic drugs
Reduced renal function
Genetic vulnerability