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TCM Exam 2 > Ambler Bacteria > Flashcards

Ambler Bacteria Flashcards

1
Q

Gram Stain

A

Hans Christian Gram, 1884

tells if bacteria are Gram ‘POS’ or ‘NEG’.

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2
Q

Gram Positives

A 
Staphylococcus
Enterococcus/Streptococcus
Bacillus
Clostridium
Listeria
Corynebacterium(Diphtheroids)
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3
Q

generalizations about spores

A

In a crux:
Bacillus and clostridium sporalate
Staph, Strep, Listeria and corynebacterium do not spore

My mnemonic: Spores, get Back in the Closet. Bacillus and Clostridium.

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4
Q

What GP has endotoxin?

A

Listeria has eNdotoxin where most GP do not

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5
Q

Staphylococcus

A

Gram Positive, catalase +, coagulase + cocci seen in Clusters.
Defenses –protein A, coagulase, hemolysins, leukocidins, penicillinase, beta-lactamases
- hyaluronidase, staphylokinase, lipase
Exotoxins

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6
Q

staphylococcus coagulase tests

A 
COAGulase POS = aureus
COAGulase NEG = rest, including
epidermidis
lugdenensis
saprophyticus
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7
Q

Staphylococcus aureus general actions

A

Can infect head to toes and pretty much everything in-between
Really NEVER considered a contaminant.
Has a extracellular vesicles that carry beta lactamase into the surroundings
ie. There is a mixed abscess with ampicillin sensitive E.coli and MRSA. Some beta lactamase inhibitor needs to be included in the treatment.

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8
Q

Staph aureus toxins

A

Enterotoxin is heat stable
TSST-1: superantigen
Coagulase
Cytolytic toxin (α toxin); pore forming toxin
Panton-Valentine leukocidin (PVL); (β toxin); pore forming toxin
seen in CA-MRSA
Exfoliatins: skin exfoliating toxin (scalded skin)

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9
Q

staph aureus symptoms/ rx

A

purulent symptoms

  • Boils
  • septic arthritis/osteomyelitis
  • Endocarditis
  • Necrotizing pneumonia (salmon tinged sputum)
  • intestinal

treatment is source control if possible
boils may only need I&D but many treat with abx as well.
bacteremia always treated with IV ABx

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10
Q

MRSA (Meth R Staph aureus)

A

MRSA almost universally put in Contact Isolation where MSSA is not.
Penicillin introduced to public 1941 (1942)
Methicillin 1958 (1960)
SCC MEC gene
CA-MRSA late 1990’s causes recurrent boils
Vancomycin 1958 (2002)
Linezolid 2000 (2001)
Daptomycin 2003 (2005)

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11
Q

VRSA

A

vanco resistant staph aureus

13 cases in US (first Detroit 2002). MI, PA, NY, DE
usually a history of recurrent MRSA and VRE skin/wound colonization with repeated vancomycin use (think dialyisis)
VAN-A gene from Enterococcus

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12
Q

VISA

A

vanco intermediate staph aureus

reduced vanco suceptibility
1997 Japan
Heteroresistance? Mixed MRSA with some VISA as well
mechanism is thickened cell wall
May confer some decrease daptomycin susceptibilities

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13
Q

Staphylococcus epidermidis

A

Indwelling foreign device
Usually more resistant than S.aureus = more methicillin R.
Vancomycin usually empiric choice
Treatment usually involves removal of foreign body.
Not typically as ‘virulent’ and not usually as purulent (no abscesses).
#1 cause of contaminated blood cultures

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14
Q

Staphylococcus saprophyticus

A

“Honeymoon cystitis” in women
Cause of UTI
Not resistant so PCN ok.

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15
Q

Enterococci

A

Historically Group D Strep so on micro reports, you may see strep species (gpc chains) that miraculously changes to enterococcus.

Clinically relevant are : faecalis and faecium

PYR + (pyrrolidonyl-arylamidase)

Highly resistant to many antibiotics
faecalis is usually less, highly resistant.
faecium is usually more, highly resistant

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16
Q

enterococci hemolysis

A

Usually gamma hemolysis

Can be alpha – especially some VRE

VRE (vancomycin resistant enterococci)

  • Difficult to treat
  • Seen in highly abx experienced patients
  • Must refer to culture sensitivity but depending on the infection and site
  • -linezolid
  • -daptomycin
  • -tetracyclines (for non-severe infections and not really urine)
  • -nitrofurantoin (for urine only)
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17
Q

VRE

A

First described Europe 1980’s. felt to be from a food additive avoparcin.

Meta-analysis of 1614 enterococcal bloodstream infections had 42% VRE which has higher mortality.

Van-A, Van-B, Van-C genes
transposon. Frightened, Promiscuous bacteria!!!
(see VRSA)

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18
Q

Streptococci

A

Different classification systems have been developed.

Rebecca Lancefield classified group A-V(no I/J) and was based on the C carbohydrate on wall

We use hemolysis as well to characterize today
catalase (-) unlike staph or other aerobes/facultative anaerobes

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19
Q

streptococcus by hemolysis

A

alpha: green pneumoniae, viridans

Beta: pyogenes, agalactiae

gamma: enterococcus

20
Q

Streptococcus pyogenes (symptoms, etc.)

A 
Group A
Pharyngitis (main cause of ‘strep throat’)
--Red, purulent tonsils
--Fever
--Lymph nodes (ant cerv)

Skin infection

  • -Folliculitis
  • -Cellulitis
  • -Impetigo (usually Staph)
  • -Necrotizing disease

Scarlet fever = fever/rash

Associated with rheumatic fever
Can cause Toxic shock syndrome 
“M” proteins (>70types) are  virulence factors
Cause of post strep glomerulonephritis
Rapidly progressing
21
Q

S. pyogenes: antibody mediated

A

Rheumatic Fever
- pharyngitis 2-4 weeks prior

Acute PSRPGN (post strep rapidly progressing glomerulonephritis)
- skin infection or pharyngitis
22
Q

Acute Rheumatic Fever

A

Major:
Migratory Arthritis (several joints in quick succession, each lasting days- 1 wk)
Carditis ( peri, epi, myo and dendo) and valvulitis (mitral stenosis as late complication)
CNS (Sydenham chorea)= St. Vitus dance
Erythema marginatum- rash
Subcutaneous nodules

Minor:
Arthralgias
Fever
Acute phase reactant elevation
Prolonged PR interval

Tx: symptomatic ie anti-inflammatories. Not proven to dec risk of rheumatic heart disease.
Abx even with negative throat culture – for stopping transmission
PCN for years to life

23
Q

Streptococcus agalactiae

A

Group B
Know this causes neonatal sepsis so pregnant moms get a screening culture antepartum
Can cause occult and sporadic disease in adults including bacteremia, SSTI, pneumonia and sepsis
esp DM
PCN DOC. Some clindamycin resistance so not reliable

24
Q

Group F = milleri group

another beta strep

A 
PUS formers including abdomen, head and neck and brain abscesses!
Think of CIA – sneaky fellows.  
Strep constellatus
Strep intermedius
Strep anginosus
25
Q

Viridans Strep

A

GI flora teeth to colon
A very large group of bacteria.
Subacute endocarditis esp post teeth cleaning
PCN DOC

26
Q

pattern, remember to treat strep with:

A

penicillin

27
Q

Streptococcus pneumoniae

A

1 bacterial cause of Community Acquired Pneumonia (20-60%), Otitis Media (29-40%) and meningitis (61%)

CAPSULE with >90 serotypes that are pathogenic.

    • Polysaccharide vaccine = 23 serotypes
    • Conjugated vaccine = 13 serotypes

Gram positive DIPLOCOCCI

28
Q

Pneumococcal treatment

A

ceftriaxone for serious infection (meningitis, bacteremia, pneumonia)
PCN still considered most potent abx
resistance starting to be a concern so with serious infection, the addition to the above with vancomycin, daptomycin, linezolid warranted prior to culture susceptibilities

29
Q

Bacillus and Clostridium

A

Spore Formers
Bacillus are more aerobic and clostridium anaerobic.
Clostridium look like ‘tennis rackets’
Bacillus look like ‘box cars’(anthrax)

30
Q

Bacillus anthracis

A

Anthrax

  • Respiratory
  • –Widened mediastinum
  • GI
  • Meningitis
  • Cutaneous most common

Potent eXotoxin

Tx: severe - antitoxin (MAB or IG), cipro, meropenum
– Less severe, pcn, clindamycin, doxy

31
Q

Bacillus cereus

A

Boards: refried rice or penetrating eye trauma

Food poisoning via heat stable enterotoxin that causes nausea/vomiting and limited diarrhea.

32
Q

Clostridium botulinum

A

Making doctors a lot of money…….
Neurotoxin that blocks release of Ach from presynaptic terminals -> flaccid muscles
Infants should not ingest natural honey prior to 6 months due to inability of GI immune system to neutralize spores. ->floppy baby
Adults usually ingest from bad canned/smoked foods.
Antitoxin and vent support.

33
Q

Clostridium tetani

A

Vaccine with Primary series and boosters q 10yrs.
Usually from skin trauma with environmental exposure.
Releases an eXotoxin called tetanospasmin that acts on inhibitory Renshaw cell interneurons preventing the release of GABA and glycine, both inhibitory substrates

34
Q

When to give tetanus shot vs. immunoglobulin

A

shot if unknown vaccination history or less than 3 doses previous or more than 10 years since last one

TIG if dirty wound and unknown vaccination history/

35
Q

Black Tar Heroin

A

Clostridium spores documented infections
necroziting disease or specific toxin disorder esp in skin poppers
- Botulism
- Tetanus
- C. sordellii also in wounds
(by the way, not common but this is in the literature for sepsis post medically induced abortion and less so spontaneous abortion. also seen post normal childbirth.)

36
Q

Clostridium difficile (C diff)

A

CDC data: 500k infections in US 2011 and 29k died within 30 days. Incidence rising and now considered most common HAI.
Risk is Antibiotics!, even what we treat with but obviously less so a risk.
Symptoms range from watery diarrhea +/- mucous to full blown septic colitis and death.
Contact isolation due to spore formation, shedding and inadvertent contamination

37
Q

Spores

A

(C Diff!)

this is the infectious problem. Found in hospital rooms on EVERYTHING (including curtains).
Patient shedding with every bowel movement and probably gasseous explosions.
put the toilet seat down to flush
spores can survive 5 months. may be dormant in colon with normal neighborhood of flora.
Patient considered infective if stool conforms to the vessel to which it is contained. Therefore contact isolation.

38
Q

C diff treatment

A

Vancomycin IV does not penetrate across colonic mucosa and oral does not really get absorbed.

Probiotics are popular with some possible benefit and little risk it appears.

Newest and greatest is Fecal Microbiota Transplantation.
Really not new but re-discovered recently.
Either it goes down from above or more commonly introduced by rectal endoscope or enema.

39
Q

Clostridium perfringens

A

Gas gangrene

Three classes

  • Cellulitis/wound: causes crepitus
  • Myonecrosis: CK elevation due to muscle dmg
  • Diarrhea: hemorrhagic necrosis of jejunum

Tx: HBO (hyperbaric O2), PCN+clinda?+IVIG?, Emergent Surgery

40
Q

Corynebacterium

A

Most are commensal flora and not pathogenic

Also reported as diphtheroids

41
Q

Corynebacteium diphtheriae

A

‘pseudomembranes’ composed of fibrin, wbc, necrotic epithelial cells

Warn micro of specimen as it requires special culture techniques
– Potassium tellurite agar and Loeffler’s coagulated blood serum media.
– TREAT 1st!
Antitoxin, PCN or erythro, and vaccinate as natural immunity may not occur.

42
Q

Listeria monocytogenes

A

Endotoxin GPR. Facultative intracellular org.
Grows in cold temperatures such as fridge
Tropism for CNS esp in young or old/immunocompromised
– also blood, amniotic fluid/placenta
3rd most common cause of neonatal meningitis.
Ampicillin 1st line. Sulfa/tmp also works

43
Q

Mycobacterium

A

acid fast staining. also weakly gram positive.
Grow on Löwenstein-Jensen media
Most are very slow growing (weeks) except Runyon Grp IV which are fast (ie 5 days)
Classified by Runyon group or Staining

44
Q

Actinomycetes

A

bacteria that act like fungi
– filamentous
– water and soil organisms
Nocardia and Actinomyces

45
Q

Nocardia

A 
Beaded, weakly GPR and weakly acid fast (but…acid fast:think mycobacterium)
no sulfur granules	
can present like sporothrix (fungus)
can disseminate including CNS infections/brain abscesses as well as pulmonary including infiltrative, nodular and cavities.  (can mimic TB).
N. asteroides
N. brasiliensis
many other species
TX: Sulfa/TMP
46
Q

Actinomyces

A 
Beaded GPR
SULFUR granules
Causes cervicfacial, abdominal infections that act like tumors.
also thoracic can look like TB or cancer
*** Historically pelvic disease associated with IUD’s.***
A. israelli
others
Tx: PCN
47
Q

Propionibacterium acnes

A

anaerobic slow growing gram positive rod; normal human skin and mucosal surface flora.

    • Acne in teens
    • surgical wounds
    • Invasive deep infections with implantable devices

becoming a real problem esp with orthopedic devices UE>LE

Painful but not necessarily inflammatory on an aspiration of a suspected joint.

Tx: PCN, Clindamycin. TCN for those acne sufferers.

TCM Exam 2 (15 decks)

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