Agrobacteriuk Flashcards

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1
Q

What is an opine

A

Low molecular weight compounds

Encoded by the T- DNA segment of DNA on an TI plasmid (agrobacterium tumerfaciens) and Ri plasmid for (Rhibozium bacteria)

Encoded on the section of DNA which is transferred into the plant genome but the adjacent vir gene present on the plasmid

They are high in carbon and nitrogen therefore can be metabolised to become a energy substrate to form essential amino acids for the bacteria to grow

They are produced by the plant genome mechanism as they have been integrated

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2
Q

What is cross viral protection

A

Prior infection of a plant with a mild strain of a virus can protect against subsequent infection with a more virulent strain

Transgenic coat protein gene of many viruses confers effective inhibition of subsequent virus infection

A commercial success of this is the papaya ringspot virus which affected the papaya industry. Using cross viral protection able to save papaya from devastating virus

Was first seen in tobacco plants which were infected with a mild strain of yellow virus the when infected with a more virulent strain immune resistance occurs

Countries like Holland is rely on this technology however we don’t do it in the Uk due to the fact it’s expensive , difficulty of delivery and the risk that the mutant strain may become virulent and infect the plants and kill large amounts

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