Afb Staining

Question 1

TB is caused by a bacterium called?

Answer 1

Mycobacterium tuberculosis

Question 2

M. tuberculosis usually attacks:

Answer 2

Kidney, spine & brain

Question 3

Two tb related condition exist:

Answer 3

Latent TB Infection
TB Disease

Question 4

How to diagnose TB disease?

Answer 4

By patients medical history, physical examination, chest x-ray & other lab tests

Question 5

2 types of sample

Answer 5

Spot-spot
Early morning -spot
Early morning -early morning

Question 6

3 important parameters to consider when coiling of sputum sample

Answer 6

Thickness
Evenness
Size

Question 7

Size of coiled sputum smear

Answer 7

2 cm x 3 cm

Question 8

It is used to stain bacteria that have high lipid content in their cell wall

Answer 8

Acid fast stain

Question 9

Where does the primary stain binds in the cell wall

Answer 9

Mycolic acid

Question 10

Color of Acid fast Bacilli & from what dye?

Answer 10

They are stained Red from carbol fuschin

Question 11

Color of Non-Acid fast bacili and what dye is used?

Answer 11

Colored as blue or green from methylene blue or malachite green

Question 12

Uses steam as a mordant

Answer 12

Ziehl-neelsen method/hot staining method

Question 13

Same reagent but higher concentration of phenol w/c serve as a chemical mordant; sometimes have a wetting agent tergitol to fasten up reaction

Answer 13

Kinyoun’s/cold staining method

Question 14

It differentiates M. Smegmatis to M. Tuberculosis

Answer 14

Pappenheim’s method

Question 15

Differentiates m. Leprae from m. Tuberculosis

Answer 15

Baumgarten’s method

Question 16

Selective for the cell wall of AFB

Answer 16

Auramine Rhodamine method

Question 17

Zhiel neelsen composed of __ % phenol & __ g carbol fucshin

Answer 17

5% and 3 grams

Question 18

Kinyoun is composed of __ g carbol fuchsin and __% phenol

Answer 18

4 g carbol fuchsin & 9% phenol

Question 19

Positive AFB

Answer 19

M. Tuberculosis ATCC 25177

Question 20

Positive -partially AFB

Answer 20

N. Asteroides ATCC 3308

Question 21

Negative- non AFB

Answer 21

E. Coli ATCC 25922

Question 22

All bacteria are non acid except:

Answer 22

Mycobacteria
Nocardia
Rhodococcus
Tsukamurella
Gordonia
Legionella micdadei

Parasite:
Isospora belli, Cryptosporidium & Cyclospora

Question 23

It renders the cells resistant to decolorization, thus termed as acid fast

Answer 23

Mycolic acid

Question 24

True or false: acid fastness is affected by colonial age, medium for growth & uv light

Answer 24

True

Question 25

True or false: kinyoun’s method is ideal for Concentrated smear and partially AFB like Nocardia spp.

Answer 25

False: Ziehl neelsen not kinyoun’s

Question 26

True or false: modified kinyoun method is not ideal for Cryptosporidia and Cyclospora parasite in stool specimen

Answer 26

False: it is recommended.