Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

BACTE > Afb Staining > Flashcards

Afb Staining Flashcards

1
Q

TB is caused by a bacterium called?

A

Mycobacterium tuberculosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

M. tuberculosis usually attacks:

A

Kidney, spine & brain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Two tb related condition exist:

A

Latent TB Infection
TB Disease

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

How to diagnose TB disease?

A

By patients medical history, physical examination, chest x-ray & other lab tests

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

2 types of sample

A

Spot-spot
Early morning -spot
Early morning -early morning

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

3 important parameters to consider when coiling of sputum sample

A

Thickness
Evenness
Size

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Size of coiled sputum smear

A

2 cm x 3 cm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

It is used to stain bacteria that have high lipid content in their cell wall

A

Acid fast stain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Where does the primary stain binds in the cell wall

A

Mycolic acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Color of Acid fast Bacilli & from what dye?

A

They are stained Red from carbol fuschin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Color of Non-Acid fast bacili and what dye is used?

A

Colored as blue or green from methylene blue or malachite green

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Uses steam as a mordant

A

Ziehl-neelsen method/hot staining method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Same reagent but higher concentration of phenol w/c serve as a chemical mordant; sometimes have a wetting agent tergitol to fasten up reaction

A

Kinyoun’s/cold staining method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

It differentiates M. Smegmatis to M. Tuberculosis

A

Pappenheim’s method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Differentiates m. Leprae from m. Tuberculosis

A

Baumgarten’s method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Selective for the cell wall of AFB

A

Auramine Rhodamine method

17
Q

Zhiel neelsen composed of __ % phenol & __ g carbol fucshin

A

5% and 3 grams

18
Q

Kinyoun is composed of __ g carbol fuchsin and __% phenol

A

4 g carbol fuchsin & 9% phenol

19
Q

Positive AFB

A

M. Tuberculosis ATCC 25177

20
Q

Positive -partially AFB

A

N. Asteroides ATCC 3308

21
Q

Negative- non AFB

A

E. Coli ATCC 25922

22
Q

All bacteria are non acid except:

A

Mycobacteria
Nocardia
Rhodococcus
Tsukamurella
Gordonia
Legionella micdadei

Parasite:
Isospora belli, Cryptosporidium & Cyclospora

23
Q

It renders the cells resistant to decolorization, thus termed as acid fast

A

Mycolic acid

24
Q

True or false: acid fastness is affected by colonial age, medium for growth & uv light

A

True

25
Q

True or false: kinyoun’s method is ideal for Concentrated smear and partially AFB like Nocardia spp.

A

False: Ziehl neelsen not kinyoun’s

26
Q

True or false: modified kinyoun method is not ideal for Cryptosporidia and Cyclospora parasite in stool specimen

A

False: it is recommended.

BACTE (3 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions