Advanced Molecular Techniques Flashcards
1
Q
How can you analyse DNA at the nucleotide level? (2 ways)
A
DNA sequencing
PCR plays restriction analysis
2
Q
How can you analyse DNA at the gene level? (5 ways)
A
Southern hybridisation Northern hybridisation RT-PCR (reverse transcriptase) Microarray DNA fingerprinting/DNA profiling
3
Q
How can you analyse DNA at the chromosome level? (2 way)
A
Karyotyping
FISH / chromosome painting
4
Q
How does reverse transcriptase PCR work?
A
Reverse transcriptase recognises poly A tail
Copies the mRNA strand and makes a cDNA strand (copy DNA)
5
Q
What is a minisatellite?
A
Tract of repetitive DNA in which certain DNA motifs are typically repeated 5-50 times
6
Q
What is FISH?
A
Fluorescent in situ hybridisation
(Colouring of chromosomes)
Can be useful for showing changes in chromosomes for example in tumours
