advanced (kinda) microscopy techniques Flashcards
1
Q
what is Phase contrast
A
it allows you to observe smaller cellular structures without staining the sample. this allows you to view living specimens.
2
Q
what is differential interference contrast microscopy
A
allows you to see contours and an almost 3d image. It works by polarising light so it hits the specimen at two different angles.
3
Q
what is wide field fluorescence microscopy
A
allows you to view fluorescent molecules whilst omitting the rest of the picture by omitting certain light frequencies. you can even view different fluorescent molecules at once.
4
Q
what is confocal laser scanning microscopy
A
allows you to see spacial relationships between structures. It gives a 3d view of the specimen
