advanced (kinda) microscopy techniques

Question 1

what is Phase contrast

Answer 1

it allows you to observe smaller cellular structures without staining the sample. this allows you to view living specimens.

Question 2

what is differential interference contrast microscopy

Answer 2

allows you to see contours and an almost 3d image. It works by polarising light so it hits the specimen at two different angles.

Question 3

what is wide field fluorescence microscopy

Answer 3

allows you to view fluorescent molecules whilst omitting the rest of the picture by omitting certain light frequencies. you can even view different fluorescent molecules at once.

Question 4

what is confocal laser scanning microscopy

Answer 4

allows you to see spacial relationships between structures. It gives a 3d view of the specimen