Adrenal Disorders Flashcards
What are the adrenal glands?
2 triangular endocrine glands which are situated superiorly on the surface of the kidney, it is divided into two sections: Adrenal cortex and medulla.
List the 3 zones of the adrenal cortex from superficial to deep and what each zone secretes.
Zona glomerulosa: Produces and mineralocorticoids secretes (aldosterone).
Zona fasciculata: Produces
and secretes glucocorticoids (cortisol); in addition to small proportions of androgens.
Zona reticularis: Produces and secretes androgens and oestrogens, as well as smaller proportions of corticosteroids.
What are the names of the cells in the adrenal medulla and what do they secrete?
Chromaffin cells which secrete catecholamines into the systemic circulation in response to stress.
Adrenaline (80%)
Noradrenaline (20%)
What is the precursor molecule for steroidogenesis?
Cholesterol is a precursor substrate used to synthesise corticosteroids:
Mineralocorticoids (Aldosterone)
Glucocorticoids (Cortisol)
Sex steroids (Androgens and oestrogen)
List the enzymes for which angiotensin-II exerts an activator effect on.
Side chain cleavage 3- beta hydroxy-steroid-dehydrogenase 21 hydroxylase 11 hydroxylase 18 hydroxylases
What enzyme cleaves cholesterol into pregnenolone and what reaction follows?
p450scc enzyme
Pregnenolone > Progesterone (3 beta-hydroxysteroid dehydrogenase)
What is the physiological purpose of aldosterone?
Stimulates potassium excretion in the urine. Promotes sodium reabsorption in the kidneys.
By promoting the renal reabsorption of sodium, aldosterone also increases water retention, exerting a direct effect on blood pressure control.
Outline the synthesis of aldosterone after progesterone is formed from pregnenolone.
Stage I - 21-hydroxylase catalyses the hydroxylation of C21 converting progesterone into 11-deoxycorticosterone.
Progesterone > 11- deoxycorticsterone
Stage II - 11-hydroxylase hydroxylates C11, converting 11-deoxycorticosterone into corticosterone.
11-deoxycorticosterone > Corticosterone
Stage III - 18-hydroxylase hydroxylates C18, converting corticosterone into aldosterone
Corticosterone > Aldosterone
Name the hormone produced by the adrenal gland that is associated with the normal stress response.
Cortisol
(Cortisol is synthesised from cholesterol, the synthesis of cortisol in the zona fasciculata is stimulated by the anterior lobe of the pituitary gland with ACTH; ACTH production is stimulated by corticotrophin release by hypothalamic parvocellular neurones.
Cortisol has a diurnal rhythm, reaching a serum peak at 9:00am.)
List the metabolic effects of cortisol
Stimulates peripheral protein catabolism; hepatic gluconeogenesis; enhances effects of glucagon and catecholamines; stimulates glycogenolysis; fat lipolysis of adipose tissue makes tissues less responsive to insulin (susceptibility to developing diabetes): Overall effect: INCREASES BLOOD GLUCOSE CONCENTRATION
Alternative effects: Mineralcorticoid, renal, CVD effects. Cortisol has the ability to bind onto mineralocorticoid receptors, therefore, behaving as aldosterone assisting with water and Na+ ion retention.
Pharmacological effects of cortisol: Anti-inflammatory action, immunosuppressive action, anti-allergic action.
What type of receptors do aldosterone and cortisol bind to respectively?
Aldosterone binds to mineralocorticoid receptors.
Cortisol binds to glucocorticoid and mineralocorticoid receptors equally.
Compare the concentration of cortisol and aldosterone in the blood.
The concentration of cortisol in the blood is approximately 1000 times greater than that of aldosterone; however, cortisol binding in inhibited.
What substance do the kidneys secrete to convert bioactive cortisol into cortisone and why?
Kidneys secrete IIB-hydroxysteroid dehydrogenase 2, converting bioactive cortisol to cortisone; constantly removing cortisol to reduce interference with mineralocorticoid receptors.
What is Cushing’s disease?
Cushing’s disease is a condition resulting from excess amounts of cortisol secretion from the adrenal cortex, primarily caused by adrenal adenomas or overstimulation by ACTH from pituitary tumours.
What is the commonest cause of Cushing’s syndrome?
Oral consumption of steroids is a common causation of Cushing’s syndrome.
List the causes of Cushing’s, separate the ACTH dependent and independent.
ACTH dependent:
Pituitary adenoma
Ectopic ACTH secretion from lung cancer
ACTH independent:
Adrenal adenoma/carcinoma
Increased ingestion of steroids by mouth
What are the symptoms of Cushing’s disease?
Weight gain (Centripetal obesity).
Reddening of the face and neck. Thick skin, fat pads (Buffalo humps), moon face, red striae and pendulous abdomen.
Excess growth of body and facial hair
Hypertension and hyperglycaemia (Impaired glucose tolerance > Diabetes)
Osteoporosis
Proximal myopathy (muscle weakness due to catabolic effects of cortisol)
Mental changes (depression).
List and explain the 4 investigations that can be done to find out if a patient has Cushing’s.
1) 24-hour urine collection for urinary free cortisol: Cortisol level >50mg/24 hours.
2) Blood diurnal cortisol levels (Highest at 9am and lowest at midnight). N.B: It is difficult to distinguish whether a patient has Cushing’s syndrome at 9am considering cortisol is at a maximum serum level. A loss of diurnal rhythm is a feature of Cushing’s.
3) Late night salivary cortisol: Should be first-line test in any patient with suspected Cushing’s Syndrome. Samples are collected by saturating a collection swab with saliva or passively drooling into a collection tube between 23:00 and midnight. Positive results should be confirmed with dexamethasone suppression or 24-hour urinary free cortisol.
4) Low dose dexamethasone test: 0.5mg; 6 hourly for 48 hours. Dexamethasone is an artificial steroid concerned with eliciting a suppressive effect on ACTH production, down-regulating the production of cortisol. Patients with Cushing’s will demonstrate
Hypersecretion of cortisol within the adrenal cortex can be inhibited using what?
11-beta hydroxylase inhibitors (Enzyme is used to convert 11- deoxycorticosterone > corticosterone and 11-deoxycortisol > Cortisol)
Give an example of an 11-beta hydroxylase inhibitor and explain its mechanism.
Metyrapone
Metyrapone inhibits 11-beta hydroxylase within the zona fasciculata and zona reticularis, arresting cortisol synthesis at the 11- deoxycortisol stage.
11-deoxycortisol has no negative feedback effect on the hypothalamus and pituitary gland.
List the unwanted actions of metyrapone and explain how they can happen.
Deoxycorticosterone accumulates within the zona glomerulosa > aldosterone-like effects (Mineralocorticoid activity) > Salt retention and HYPERTENSION.
Using metyrapone can lead to increased adrenal androgen production (Hirsutism in women).