Adhesives & Mounting

Question 1

Used in order to promote adhesion of sections to
slides

Answer 1

ADHESIVES

Question 2

ADHESIVES is spread thinly and evenly on a clean ____ glass slide which is then gently approximated to the end of the __ and drawn _____ in a near _____ motions

Answer 2

Spread thinly and evenly on a **clean grease-free
glass slide ** which is then gently approximated to
the end of the ribbon and drawn upwards in a near vertical motions

Question 3

Most commonly used because it is very easy to make and relatively inexpensive

Answer 3

Mayer;s Egg Albumin

Question 4

Mayer;s Egg Albumin formula (egt💩= 100 )

Answer 4

Egg white (50 mL)
Glycerin (50 mL)
Thymol Crystals (100 mg)

Question 5

Dried albumin formula (the std🦠)

Answer 5

Dried albumin (5 g)
Sodium Chloride (5g)
Thymol crystals
Distilled Water (100mL)

Question 6

1% Gelatin formula (great dogs grow playfully)

Answer 6

Gelatin (1 g)
Distilled Water (100mL)
Glycerol (15 mL)
Phenol crystals (2 g)

Question 7

1% Gelatin:
Gelatin (__g)
Distilled Water (___ mL)
Glycerol (___ mL)
Phenol crystals (___ g)

Answer 7

Gelatin (1 g)
Distilled Water (100mL)
Glycerol (15 mL)
Phenol crystals (2 g)

Question 8

Gelatin formula (gf👯‍♀️)

Answer 8

1 % Gelatin (5 mL)
2 % Formaldehyde (5 mL)

Question 9

Gelatin formula
1 % Gelatin (__ ml)
2 % Formaldehyde (__ ml)

Answer 9

1 % Gelatin (5 mL)
2 % Formaldehyde (5 mL)

Question 10

Starch Paste formula (kalawmon🕳️)

Answer 10

Distilled water (30 mL = 10 mL cold, 20 mL boiling)
Powdered starch (1 g)
Thymol crystals
Hydrochloric acid (2 drops)

Question 11

Starch paste formula measurements:

Distilled water (__ml = __ cold, __ boiling)
Powdered starch (___g)
Hydrochloric acid (___ drops)
Thymol crystals

Answer 11

Distilled water (30 mL = 10 mL cold, 20 mL boiling)
Powdered starch (1 g)
Hydrochloric acid (2 drops)
Thymol crystals

Question 12

readily available from outdated blood stored in blood banks, dispensed into sterile tubes of 0.5 mL each

Answer 12

Plasma

Question 13

Used as a section adhesive in immunohistochemistry

Answer 13

Poly-L-Lysine

Question 14

Poly-L-Lysine is a ___ % ____ solution which is further diluted ___ with distilled water prior to use

Answer 14

0.1 % aqueous detergent solution which is further diluted 1:10 with distilled water prior
to use

Question 15

Final dilation of Poly-L-Lysine

Answer 15

0.01%

Question 16

very useful in cytology particularly for cytospin
preparations of proteinaceous or bloody material

Answer 16

3-APES (3-Aminopropyltriethoxy silane)

Question 17

A syrup fluid applied between the section and
the coverslip after staining setting the section
firmly, preventing the movement of the coverslip

Answer 17

Mounting Medium AKA: Mountant

Question 18

For best results with stained tissue sections, mounting medium must be impregnated by a transparent medium having RI (refractive index) close to that of ___

Answer 18

glass (1.518)

Question 19

designed to mount water-miscible
preparations directly from water in cases
where the stains is removed or
decolorized with alcohol or xylene

Answer 19

Aqueous/Water Soluble Mountains

Question 20

Types of Aqueous/Water Soluble Mountains (WeGotFABulous!!!)

Answer 20

WATER
Glycerin
Farrant’s medium (Gum syrup)
Apathy’s medium
Brun’s fluid

Question 21

• Low refractive index
• Good only for temporary mounting

Answer 21

water

Question 22

Glycerin refractive index

Answer 22

1.47

Question 23

• Also used as preservative
• Has high index of refraction

Answer 23

Glycerin (RI: 1.47)

Question 24

Farrant’s medium (Gum syrup) refractive index

Answer 24

1.43

Question 25

does not solidify upon storage

Answer 25

Farrant’s medium (Gum syrup) (RI:1.43)

Question 26

used for methylene blue-stained nerve
preparations and as general purpose
aqueous mountant

Answer 26

Apathy’s medium

Question 27

recommended for mounting frozen sections from water or paraffin sections which require dehydration and clearing

Answer 27

Brun’s fluid

Question 28

used for preparations that have been
dehydrated and cleared in xylene or
toluene and are recommended for
majority of staining methods

Answer 28

Resinous Mountants

Question 29

Canada Balsam refractive index

Answer 29

1.524

Question 30

Recommended for whole mounts and
for thick sections because it does not
shrink much

Answer 30

Canada balsam

Question 31

Refractive index of DPX

Answer 31

1.532

Question 32

XAM refractive index

Answer 32

1.52

Question 33

Recommended for small tissue sections

Answer 33

DPX (RI: 1.532)

Question 34

Synthetic resin mixture in xylene in pale yellow or colorless solution

Answer 34

XAM (RI: 1.52)

Question 35

Clarite refractive index

Answer 35

1.544

Question 36

Synthetic resin which is soluble in xylene (usually diluted to 60% with xylene)

Answer 36

Clarite (RI: 1.544)

Question 37

(2) TWO MOUNTING MEDIA MOST COMMONLY
USED TODAY

Answer 37

Eukitt
Entellan

Question 38

process of sealing the margins of the
coverslip to prevent escape of fluid or semi-fluid
mounts and evaporation of mountant, to
immobilize the coverslip and to prevent sticking
of slides upon storage

Answer 38

RINGING

Question 39

Kronig cement is made up of 2 parts ___ mixed with 4-9 parts powdered ____, heated and filtered

Answer 39

made up of 2 parts paraffin wax mixed with 4-9
parts powdered colophonium resin, heated and
filtered