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What is a whip like tail called on a bacteria
A flagellum
How does a flagellum help bacteria
It helps them move
Function of the nucleus
Contains genetic material controlled the activities of the cell
Function of the cytoplasm
Most chemical processes take place here, controlled by enzymes
Function I the cell membrane
Controls movement of substances in and out of the cell
Function of the mitochondria
Most energy is released through respiration here
Function of ribosomes
Protein synthesis happens here
What part of cells produce protein
Ribosomes
What is the function of cell walls
Strengthens plant cells
What is the function of chloroplasts
Contains chlorophyll which absorbs light energy for photosynthesis
What is the function of the permanent vacuole
Filled with cell sap to help keep the cell turgid
What does turgid mean in a biology context
Enlarged and awoken with water
Which two inventors are credited for creating the first microscope
Hand and saccharides Janssen
What was the problem with early microscopes
They did not magnify to a great extent
How many times can modern light microscopes magnify
1500 times
How many times can an electron microscope magnify
Two million times
How has the improvement of microscopes led to scientific discoveries
Has allowed scientists to see small things such as cells in much greater detail
As a result our understanding of their function has improved
How can we calculate the length of a magnified object
Length of object=length of magnified object/magnification
What are enzymes
Large molecules that speed up the chemical reactions inside cells, each type of enzyme does one specific job
What are enzymes
Long chains of protein
What are proteins made of
Long chains of amino acids
What is aerobic respiration
Where does it take place.
Aerobic desperation is our main source of energy
It takes place in the mitochondria
What are mitochondria
What’s in them
Where are they in the body
Oval structures with a folded inner membrane
They have a large number of different enzymes inside them. Responsible for different stages of respiration
They are found in large numbers in places like the liver and muscles-places that need a lot of energy
What are enzymes
Soluble protein molecules that speed up chemical reactions
What are the compact structures which contain DNA
Chromosomes
What is the shape of DNA
The double helix
How are the two strands of a DNA double helix held together
Hydrogen bonds between pairs of bases
How does a cell make a duplicate copy of each DNA molecule
The bonds between the two strands of DNA break. The strands unwind new bases then join each old strand to make two new double helix eps
What are the three steps of the duplication of the old DNA helix
DNA spiral unzips
Free nucleotides lock onto bases
Two copies of the original spiral are formed
How do the order of the bases in DNA control protein
Each amino acid has its own code of three bases, each time the same three bases are in the genetic code the same amino acid is added to the chain to make a protein
What is the experiment for extracting DNA from cells
Peas
Salt water and detergent are added to mashed up peas
This mixture is left at sixty degrees for fifteen minutes
Then filter the mixture
Pour iced ethanol on the filtrate, the DNA will float to the surface
What are the steps in order to the discovery of the DNA molecule
Rosalind franklin and Maurice Wilkins studied and photod X-rays of DNA
James Watson and Francis crick worked out the three d structure of the molecule
Everyone except for franklin won Nobel prizes because she died
What was the human genome project
A project to find the order of all the three billion base pairs in humans
To develop faster methods for sequencing DNA
When was the human genome sequencing finished
Two thousand and one
Why did the human genome project use several people
To get an average sequence
Scientists from how many countries collaborated to work on the Hgp
18
How is the human genome project useful
Improved genetic testing
Location of genes which are linked to encreased chance of inheriting a disease
New gene therapy treatments
New knowledge of how humans have evolved
Personalised medicines
Who invented genetic fingerprinting
Sir alleviated Jeffrey’s
How does genetic fingerprinting work
It uses small genetic differences to make a picture like a bar code
What are the two differences between cloning and genetic engineering
Cloning
Produces exact copy’s
Genes are copied within the same species
Genetic engineering
Ptoduces a unique set of genes
Genes can be swapped across species
How gm works
Enzymes cut DNA containing a certain gene from one organism
And join them into a gap in the DNA of another organism
What is an example of genetic modification
Bacteria modified to produce insulin
How are bacteria modified to produce insulin
Steps.
Restriction enzyme cuts out the gene that produces insulin
Enzyme cuts bacteria plasmid an inserts human insulin gene into the gap
Bacteria produces insulin
Three examples of genetically modified plants and animals to vena fit humans
Gm insulin
Golden rice produces extra beta carotene used to make vitamin a
Herbicide resistant crops
Positives of gm insulin
Is easier to create high quantities
Less likely to cause an adverse reaction
Overcomes ethical concerns from vegetarians
Negatives of golden rice
Fears it will cross breed with wild rice
Worries that gm organisms might harm people
Beta carotene levels aren’t high enough to make a difference
Are expensive
Possible disadvantages to herbicide resistant crops
Potential development of herbicide resistant weeds
Loss of biodiversity as fever weeds survive to provide shelter for animals
Who invented the periodic table
Dmitri Mendeleev
What are the two types of DNA in bacteria called
Plasmid and chromosomal
What’s the benefit of humans being multi cellular
Three
Cells can be specialised to do certain things
Groups of cells can function as organs making a more efficient but complex organism
The organism can grow very large
What does it mean if a cell is diploid
It has two of each chromosome
What is mitosis
The type of cell division used for growth repair and asexual reproduction
What does mitosis produce
Two cells that are identical to each other and it’s parent cell
What are the four steps of mitosis
Chromosomes in nucleus are copied.
Chromatids pulled apart and moved towards poles
Chromosomes separate
Cell divides into two genetically identical daughter cells
Why is cell division in multi cellular organisms necessary
The larger the cell becomes the smaller it’s surface area to volume ratio becomes. Objects with a small range find it difficult to maintain exchange of materials with their environment. Large cells could run out of oxygen and accumulate too much waste like co2
What is all reproduction in humans
Sexual
What is a haploid cell
When a cell has half the usual amount of genetic material
What are the cells that combine to form the zygote called
Gametes
How many chromosomes do human zygotes contain
46
How many chromosomes do human gametes contain
23
What is the type of cell division that produces gametes called
Meiosis
What is responsible for causing genetic variation
Meiosis
What is a gamete
A cell with half the normal amount of chromosomes only used for reproduction
What is a zygote
Cell formed when two gametes combine
What is fertilisation in reproduction
Term to describe the joining of two gametes
What is a haploid
Having half the normal amount of chromosomes
Diploid
Having the normal amount of chromosomes
What are adaptions to the sperm gamete to help it perform it’s job
They have a tail to propel them
Many mitochondria for energy
The front of the sperm contains enzymes to digest the egg membrane
What are adaptions to the egg gamete to help it perform it’s job
Has a large food store to support the developing zygote until it can feed from the placenta
7 steps of meiosis
Parent cell
Chromosomes make identical copy’s of themselves
Similar chromosomes pair up
Sections of DNA get swapped
Pairs of chromosomes divide
Chromosomes divide
Four genetically different haploid daughter cells produced
Which process of cell division is used in asexual reproduction
Mitosis
Which plants can do asexual reproduction
A potato plant can many tubers which each of which can grow into a new plant
Strawberry and spider plants produce long stems with tiny plants on the end these runners can produce several new plants from one parent
What are the steps of cloning
Removal of a diploid nucleus from a body cell
Emu elation of an egg cell (take out the nucleus)
Insertion of diploid nucleus into the emu cleated egg cell
Electrical stimulation of the diploid nucleus to divide by meiosis
What is an example of cloning
Dolly the sheep
Ethical issues surrounding cloning
Two
Restriction of cloning humans also restricts scientific research
Cloning plants is done all the time and causes the public less ethical and moral concerns
How are cows cloned using embryo transplants
5 steps
Sperm is taken from a bull in a high yield dairy herd
Cow is artificially inseminated with sperm
Zygotes develop into embryos in cow and then removed from uterus
Embryos are split into several smaller embryos each of which can grow into a new calf
Embryos are placed into the uteruses of foster mums
How would we go about genetically modifying pigs to grow human organs
The pigs would have human genes inserted into their cells so the organs would not be rejected when transplanted into human bodies
Once the insertion of human genes had been done we would clone the pig as this is less hard than inserting human genes
Two disadvantages to cloning
If a clone is susceptible to disease or changes in environment then all the clones will be susceptible
It will lead to less variation and less opportunity to create new varieties in the future
Two advantages to cloning
All the new organisms are identical- they will all have the desired characteristics
Organisms that are difficult or slow to breed can be reproduced very quickly. Some plants do not produce seeds others lie dormer for a long time
4 things stem cells can be used for
Making new brain cells to treat people with Parkinson’s
Rebuilding bones and cartilage
Repairing damaged immune systems
Making replacement heart valves
What is therapeutic cloning
Cloning one of your cells to produce an embryo then taking stem cells from this for your use
What are the six steps to therapeutic cloning
Nucleus taken out of human egg cell
Nucleus from patients cell put into the egg cell
Egg cell stimulated to develope into an embryo
Stem cells taken from the embryo
Stem cells grown in a container of warm nutrients
Stem cells treated to grow into required cell types
To enable genes to code for proteins the bases Atgc get to get her in triplets
Each protein is made up of large numbers of amino acid molecules
Each triplet of bases codes for an amino acid
Amino acids are made in the order and number dictated by the base triplets
Amino acids join together a long chain to make a protein molecule the number and sequence of amino acids determines which protein results
What is transcription
The first part of the process of making protein
It takes place inside the cell nucleus
Transcription involves copying the DNA
What are the steps of transcription
The DNA unzips so that both genes are separate one strand is used as a template
Complimentary bases attach to the strand being coded
Thymine base t is not present and a different bace u joins with a in the way that t would have done
This forms a strand of messenger RNA
What replaces t in transcription
U
What do ribosome’s do
Make protein to be used inside the cell
What takes place in translation
This is when the messenger RNA is ‘interpreted’
What are the stages of translation
6
The mRNA attaches to a ribosome
The ribosome reads the mRNA
The ribosome decodes the mRNA in groups of three
Base triplets or codons which are complimentary to bases in transfer RNA
The tRNA is specific to an amino acid that collects and returns to the mRNA
Bonds form between the amino acids and polypeptide Is formed
The polypeptide chain folds up and becomes a specific shape forming a protein
What is the part of the enzyme molecule that matches the substrate called
The active site
What is the chemical that an enzyme reacts with called
The substrate
How fast are enzymes reactions at cold temperatures
Slow
Do enzymes reactions speed up as they heat up
Up to a point
After the optimum temp has been passed it slows down the reaction speed
What ph do most enzymes work best at
Neutral conditions
What is the relationship between substrate concentration and enzyme reaction speed
The higher the concentration of substrate the faster the reaction-up to a point
What is it called when enzymes break down due to heat
Denaturing