ACTIVITY 4: MEDIA COMPOSITION AND CLASSIFICATION OF MEDIA

Question 1

According to ______, ______, and ______ (2005), a ______ is a solid or liquid preparation used to ______, ______, and ______ microorganisms.

Answer 1

Prescott, Harley, Klein, culture medium, grow, transport, store

Question 2

Specialized ______ are essential in the ______ and ______ of microorganisms, the testing of ______, ______ and ______ analysis, ______ microbiology, and other activities.

Answer 2

media, isolation, identification, antibiotic sensitivities, water, food, industrial

Question 3

The use of ______ is an essential technique in assessment and isolation of specific microorganisms found in food.

Answer 3

culture media

Question 4

Generally, there are four types of media, if they are categorized as to their purpose in microbiological analysis:

Answer 4

General purpose media
Selective media
Differential media
Enrichment media

Question 5

Generally, there are four types of media, if they are categorized as to their purpose in microbiological analysis.

______ such as ______ and ______ are categorized as such because they support the growth of many microorganisms; and are usually used for heterotrophic plate counts.

Answer 5

General purpose media, tryptic soy broth, tryptic soy agar

Question 6

Generally, there are four types of media, if they are categorized as to their purpose in microbiological analysis.

______ are named as such since they contain salts or dyes which favor growth of particular microorganisms. They are usually used to detect the presence of selected types of microorganisms such as ______ and ______ for gram negative enteric bacteria.

Answer 6

Selective media, eosin methylene blue agar, McConkey agar

Question 7

Generally, there are four types of media, if they are categorized as to their purpose in microbiological analysis.

______ on the other hand, are media which can be used to distinguish between different groups of bacteria and may even permit tentative identification of microorganisms based on their biological characteristics. Example, appearance of a clear halo around colonies of hemolytic bacteria in ______.

Answer 7

Differential media, blood agar

Question 8

Generally, there are four types of media, if they are categorized as to their purpose in microbiological analysis.

______ are media which promote the growth of a particular organism by providing it with essential nutrients and rarely contain certain inhibitory substances to prevent the growth of normal competitors. Example, addition of blood, serum, or egg to general media to encourage the growth of specific microorganisms.

Answer 8

Enrichment media