ACTIVITY 1: WET MOUNT PREPARATION AND THE HANGING DROP METHOD Flashcards
______ is important in the primary characterization of organisms, be it from a ______ or from a ______ medium.
Microscopy, sample, culture
Many techniques are employed using ______ which aid in description and identification of ______ and ______.
staining methods, bacteria, fungi
______ and ______ are usually used to observe live microorganisms and to determine ______.
Wet mounts, hanging drops, motility
In a ______, the specimen is suspended in a drop of liquid (usually ______) located between ______ and ______.
wet mount, water, slide, cover glass
In the ______, the specimen is suspended in the form of a drop usually in a ______ slide or its improvised version.
hanging drop, depression
The ______ of the water improves the image quality and also supports the specimen.
water refractive index
In contrast to permanently mounted slides, ______ and ______ cannot be stored over ______ periods, because they tend to ______.
wet mounts, hanging drops, extended, dry out
Wet mounts and hanging-drop preparations allow observation of living microorganisms in their ______, helping to assess ______, ______, and ______ without distortion from ______ or ______.
natural state, motility, shape, behavior, staining, heat fixation
The ______ is quick and easy, while the ______ prevents drying, enabling ______ study.
wet mount, hanging-drop method, extended
These techniques are crucial in microbiology, especially in distinguishing ______ from ______ bacteria, and enhance ______ due to the water’s ______.
motile, non-motile, visibility, refractive index
Dropping the coverslip abruptly can trap ______, distort the ______, and displace ______, making them difficult to observe. The sudden ______ may also damage delicate structures like ______ or ______.
air bubbles, view, microorganisms, pressure, flagella, fungal hyphae
Uneven ______ can create areas too ______ or ______, leading to inconsistent ______ and ______. To avoid this, the coverslip should be lowered gently at an ______ to allow even ______ of the specimen.
spreading, thick, thin, focus, lighting, angle, distribution
______ can distort light and obscure microorganisms.
Air bubbles
To remove air bubbles, gently ______ on the coverslip’s ______ with a ______ or ______, ______ the coverslip slightly, or add a small drop of ______ near the edge to push bubbles out.
press, edge, needle, loop, tilt, liquid
Ensuring proper ______ when placing the coverslip helps prevent ______ in the first place.
technique, bubbles
______ are prokaryotic, unicellular microorganisms that lack a ______ and ______ organelles.
Bacteria, nucleus, membrane-bound
______ are the smallest among the three groups, typically ranging from ______ to ______ micrometers in size.
Bacteria, 0.2, 2
Bacteria have ______ structures and reproduce rapidly through ______.
simple, binary fission
Bacteria can have various shapes—______ (spherical), ______ (rod-shaped), and ______ (spiral-shaped)—and some possess ______ for motility.
cocci, bacilli, spirilla, flagella
Bacteria can be found in diverse environments, from ______ and ______ to the ______, and can be beneficial (e.g., ______) or harmful (e.g., ______).
soil, water, human body, gut microbiota, pathogenic bacteria
______ are eukaryotic microorganisms, meaning they have a nucleus and organelles.
Protists
______ are mostly unicellular, though some form colonies.
Protists
______ are typically larger than bacteria (______-______ micrometers) and exhibit greater complexity.
Protists, 10, 100
Protists are highly diverse, including ______ (animal-like protists), ______ (plant-like protists), and ______ (fungus-like protists).
protozoa, algae, slime molds
