active transport lab Flashcards
define active transport?
process that moves solutes through the cellular membrane using energy in atp form
define passive transport
transport molecules moving through concentration gradient and lipid bilayer by simple diffusion
what is primary active transport
it is like active transport where energy is used to pump which what goes against the concentration gradient
what is 2nd active transport
transport of a solute in the direction of its increasing electrochemical potential coupled to the facilitated diffusion of a second solute (usually an ion) in the direction of its decreasing electrochemical potential.
Mucosal side is referred to as _____ of an epithalial Membrane and Serosal Side is ____side of the membrane
a. external
b. internal
Which side of the frog skin is selectively permeable to Na+ ion?
mucosal `
Which drugs we are going to use in this experiment?
Amiloride and Ouabain
According to ohm’s law, Current is equal to:
voltage/resistance
what is ouabain and why is it used in this lab?
it is a drug used for cardiac glycoside used for hypertension, it is used due to inhibit na+/k+ activity altering musical side by changing na+flux and ISC
What is amiloride and why is it used in this lab
it is an inhibitor drug of passive transport, and it is used to see if it will cause differ ISC and na+ influx compared to predrug mucosal.
what does the Na-K ATPase Pump do?
expels sodium ions from the cells and brings potassium ions in IMPORTANT FOR PRIMARY ACTIVE TRANSPORT
in frog skin what occurs when you change the current
high= more na+ flux
lower= less Na+ flux
what side is permeable to na+ but not to k+
mucosal
what is the short iurcuit current (ISC)
current requires to short circuit the skin, It is measured as micrometer, and measured by the active of na+/k+-atpase pumps
