Act 9: Total CK Flashcards
Creatine kinase (CK) is primarily found in (3)
- skeletal muscle
- cardiac muscle
- brain tissue
Creatine kinase (CK) is primarily found in skeletal muscle, cardiac muscle and brain tissue. Damage to any of these tissues may result in?
increased levels of CK activity in serum.
Cardiac muscle damage following myocardial infarction usually results in an increase to 7-12
times the upper limit of normal within ____ to ______ hours of infarction.
18 to 30 hours
Elevated CK activity is also noted in?
- hypothyroidism
- various types of muscular dystrophy
- viral myositis
- similar types of skeletal muscle diseases
The determination of serum CK activity is used to aid in the diagnosis of? (2)
- myocardial infarction
- various types of muscle disease
The first procedure for determining CK, based on the rate of ATP formation, was presented by ____ in _____.
Oliver in 1955
The first procedure for determining CK, based on the __________, was presented by Oliver in 1955.
rate of ATP formation
A modified method was described by ______ and ______ in ____ with Rosalki adding a sulfhydryl compound and AMP in 1967 to assure maximum CK
activity and inhibit adenylate kinase activity.
Nielson and Ludvigsen in 1963
A modified method was described by Nielson and Ludvigsen in 1963 with _______ adding a sulfhydryl compound and AMP in ______ to assure maximum CK
activity and inhibit adenylate kinase activity.
Rosalki; 1967
Optimized conditions for measuring CK were
published by ______ in ______ as well as by the ______ on Enzymes.
Szasz in 1976; Scandinavian Committee
Optimized conditions for measuring CK were
published by Szasz in 1976 as well as by the Scandinavian Committee on Enzymes. The
above procedure was modified again in _____ to include ______.
1979; EDTA
Principle of Total CK
CK
ADP + Creatine Phosphate——————————->3⁄4 Creatine + ATP
HK ATP + Glucose ------------------------------->3⁄4 ADP + Glucose –6-Phosphate
G6PDH G6P + NAD+ ----------------------->3⁄4 6-Phosphogluconate + NADH + H+
The rate of NADH formation, measured at _______, is directly proportional to serum CK activity.
340 nm
Calculations for Total CK
= ΔAbs./min x 6592
Total reaction volume in Total CK
1.025
