Act 8: Amylase

Question 1

The determination of amylase activity in serum and urine is most commonly performed for the diagnosis of

Answer 1

acute pancreatitis.

Question 2

In acute pancreatitis, amylase levels are elevated for longer periods of time in ______ than in ______

Answer 2

urine than in serum

Question 3

Amylase was first measured quantitatively by an?

Answer 3

iodometric method introduced by Wohlegemuth in 1908

Question 4

______ introduced a procedure in 1938 which standardized the amounts of starch and iodine.

Answer 4

Somogyi

Question 5

Disadvantages of these methods include (3)

Answer 5

long incubation times
endogenous glucose interference
unstable reaction colors

Question 6

Several procedures have been suggested, including one which uses the defined substrate _______

Answer 6

maltotetraose

Question 7

Wallenfels et al introduced ____________ as defined substrates for amylase determination in a procedure that eliminated interference from endogenous glucose and pyruvate.

Answer 7

p-Nitrophenylglycosides;

Question 8

The present procedure is based on modifications of Wallenfels, using as substrate __________with the terminal glucose blocked to reduce spontaneous degradation of the substrate by glucosidase and glucoamylase.

Answer 8

pNitrophenyl-D-Maltoheptaoside (PNPG7)

Question 9

PRINCIPLE of amylase

Answer 9

See manual

Question 10

The rate of increase in absorbance is measured at ______ and is proportional to the amylase activity in the sample.

Answer 10

405 nm

Question 11

Millimolar absorptivity of p-Nitrophenol

Answer 11

8.5

Question 12

Amylase is multiplied to?

Answer 12

4824

Question 13

Then PNPG1 is hydrolyzed by glucosidase to glucose and pNitrophenol, which produces a?

Answer 13

Yellow color