Act 7: LDH

Question 1

Increased levels of LD are associated with

Answer 1

myocardial infarction
liver disease
pernicious anemia
in some cases of renal disease
in some cases of skeletal muscle trauma

Question 2

Levels of LDH reach a maximum at?

Answer 2

48 hours after the onset of pain and persist about ten days

Question 3

published the first UV kinetic method for the determination of LDH activity in serum in 1955

Answer 3

Wroblewski and Ladue

Question 4

Wroblewski and Ladue published the first UV kinetic method for the determination of LDH activity in serum in 1955. Their method was based on the?

Answer 4

classic Kubowitz and Ott assay (1943)

Question 5

classic Kubowitz and Ott assay (1943) utilizing the?

Answer 5

pyruvate to lactate reaction

Question 6

described a procedure that followed a lactate to pyruvate reaction.

Answer 6

Wacker et al

Question 7

The lactate to pyruvate reaction became the preferred reaction because?

Answer 7

because of a wider linear range and no pre-incubation requirement

Question 8

PRINCIPLE of LDH

Answer 8

See manual

Question 9

The rate of NAD reduction can be measured as an increase in absorbance at

Answer 9

340 nm

Question 10

Change in absorbance

Answer 10

(A2-A1)

Question 11

Total reaction volume in ml

Answer 11

1.05

Question 12

Conversion of U/ml to U/L

Answer 12

1000

Question 13

Millimolar absorptivity of NADH

Answer 13

6.22

Question 14

NORMAL VALUES: LDH
Male:

Answer 14

50-166 U/L (30°C)

80-285 U/L (37°C)

Question 15

NORMAL VALUES: LDH
Female:

Answer 15

60-132 U/L (30°C)
103-227 U/L (37°C)

Question 16

LDH calculation

Answer 16

(A2-A1)= abs/min x 3376

Question 17

Calculation of amylase

Answer 17

Abs./min x 4824

Question 18

NORMAL VALUES: amylase serum:

Answer 18

up to 96 IU/L

Question 19

NORMAL VALUES: amylase urine

Answer 19

18 to 330 IU/L