Acids and Bases Part 3 Flashcards
TRUE or FALSE
Ventilation affects the pH of the blood
TRUE
TRUE or FALSE
Ventilation do nothing to the pH of the blood
FALSE
Ventilation affects the pH of the blood, this system is called the “respiratory”
What system does ventilation affects the pH of the blood?
respiratory
Components of acid-base balance.
respiratory and nonrespiratoryor metabolic component of acid-base balance.
Controls bicarbonate concentration
Renal system
The overall reaction results in the reabsorption of sodium and bicarbonate in the kidney tubules.
Renal system
Fill in the blanks.
The overall reaction results in the reabsorption of _________ in the kidney tubules.
sodium and bicarbonate
These substances pick up excess hydrogen ions.
nonrespiratory or metabolic
How does the lungs regulate pH?
through retention or elimination of CO2 by changing the rate and volume of ventilation.
How does the kidneys regulate pH?
by excreting acid, primarily in the ammonium ion, and by reclaiming HCO₃⁻ from the glomerular filtrate.
Fill in the blanks.
The kidneys regulate pH by excreting acid, primarily in the ammonium ion, and by reclaiming HCO₃⁻ from the ________.
glomerular filtrate
Fill in the blanks.
The kidneys regulate pH by excreting acid, primarily in the _____, and by reclaiming HCO₃⁻ from the glomerular filtrate.
ammonium ion
Fill in the blanks.
The kidneys regulate pH by excreting acid, primarily in the ammonium ion, and by reclaiming _____ from the glomerular filtrate.
HCO₃⁻
Fill in the blanks.
The lungs regulate pH through retention or elimination of CO2 by changing the rate and __________ .
volume of ventilation
An acid-base disorder that results from hypoventilation
Respiratory acidosis
Which acid-base disorder causes a decrease in carbon dioxide elimination?
Respiratory acidosis
Compensation occurs by the kidneys increasing the resorption of bicarbonate.
Respiratory acidosis
An acid-base disorder results from an increase in ventilation.
Respiratory alkalosis
An acid-base disorder resulting in excessive elimination of carbon dioxide.
Respiratory alkalosis
Compensation occurs by the kidneys excreting more bicarbonate.
Respiratory alkalosis
An acid-base disorder that occurs in many disorders and results in a decrease in bicarbonate levels.
Nonrespiratory (metabolic) acidosis
The lungs compensate by hyperventilating.
Nonrespiratory (metabolic) acidosis
Is produced in many disorders and results in an increase in bicarbonate levels.
Nonrespiratory (metabolic) alkalosis
The lungs compensate by hypoventilation.
Nonrespiratory (metabolic) alkalosis
Migration of charged particles in some medium (either liquid or solid) when an electrical field is applied.
Electrophoresisis
Used clinically to separate and identify proteins
Electrophoresisis
Includes serum, urine and cerebrospinal fluid (CSF) proteins, lipoproteins, isoenzymes, and so on.
Electrophoresisis
Migrate toward the positive electrode (anode)
negatively charged particles
migrate toward the negative electrode (cathode)
positively charged particles
positive electrode
anode
negative electrode
cathode
TRUE or FALSE
Positive electrode is cathode.
False
Positive electrode is anode.
TRUE or FALSE
Negative electrode is anode.
False
Negative electrode is cathode.
The movement of charged molecules in a porous supporting medium where the molecules(macro) separate as distinct zones.
Zone electrophoresis
Refers to the migration of small ions
Iontophoresis
What charged particles will migrate towards the anode (+electrode).
Negatively charged particles
What charged particles will migrate towards the cathode (-electrode).
Positively charged particles
Directly proportional to rate of movement
Charge of the molecule
Inversely proportional to rate of movement
Size of the molecule
Increased current increases migration rate
Electrical field
Increased ionic strength decreases migration rate
Ionic strength of buffer
Decreased pH slows migration
pH of buffer
Inversely proportional to migration rate
Viscosity of supporting medium
High temperature can denature protein and slow migration
System temperature
Paper electrophoresis use has been replaced by cellulose acetate or agarose gel in clinical laboratories.
Cellulose Acetate
What electrophoresis has been replaced by cellulose acetate or agarose gel?
Paper electrophoresis
Widely used supporting medium
Agarose Gel
Only requires small amounts of sample (approx. 2 mL)
Agarose Gel
Polyacrylamide gel electrophoresis involves separation of protein on the basis of charge and molecular size.
Polyacrylamide Gel
Involves separation of protein on the basis of charge and molecular size.
Polyacrylamide gel electrophoresis
Same with polyacrylamide
Starch Gel
TRUE or FALSE
Separated protein fractions are stained to reveal their locations.
TRUE
TRUE or FALSE
Different stains come with different plates from same manufacturers.
FALSE
different manufacturers.
ELECTROPHORESIS
Stains used for visualizing serum proteins
Amido black B, Ponceau S, and Coomassie brilliant blue
ELECTROPHORESIS
Amido black B, Ponceau S, and Coomassie brilliant blue stains are used for visualizing what?
Serum proteins
ELECTROPHORESIS
Used for CSF proteins
Silver nitrate
ELECTROPHORESIS
Used for lipoproteins
fat red 7B and oil red O
What are fat red 7B and oil red O used for?
Used for lipoproteins
Used for lactate dehydrogenase isoenzymes.
nitrotetrazolium blue
TRUE or FALSE
Nitrotetrazolium blue is used for lactate dehydrogenase isoenzymes.
TRUE
TRUE or FALSE
Silver nitrate is used for lipoproteins
FALSE
Silver nitrate is used for CSF proteins
Simplest way to accomplish detection is visualization under ?
UV light
TRUE or FALSE
Simplest way to accomplish detection is visualization under moon light.
FALSE
Simplest way to accomplish detection is visualization under UV light.
Most common and reliable way for quantitation.
densitometry
What percentage does a human body contains?
66% mass of a person is water
What percentage is earth covered by water?
71% is covered by water
What percentage is earth covered by water in ice?
3% is ice
TRUE or FALSE
Water is needed to sustain all living matters.
TRUE
TRUE or FALSE
Water is not needed to sustain all living matters.
FALSE
What is the importance of blood gas homeostasis?
To assess acid-base balance
TRUE or FALSE
When pH increases, it denotes alkalinity, it needs to produce acid to meet the normal level.
TRUE
TRUE or FALSE
Acidosis causes acidemia
TRUE
TRUE or FALSE
Alkalosis causes alkalemia
TRUE
TRUE or FALSE
Alkalosis causes acidemia
FALSE
Alkalosis causes alkalemia
TRUE or FALSE
Acidosis causes alkalemia
FALSE
Acidosis causes acidemia
Able to carry metabolic waste or waste products
RBC
Protein that binds oxygen
hemoglobin
If haven’t transported the oxygen
oxyhemoglobin
TRUE or FALSE
Numerator denotes kidney function
TRUE
Denominator denotes lung function
TRUE
TRUE or FALSE
Numerator denotes lung function
FALSE
Numerator denotes KIDNEY function
TRUE or FALSE
Denominator denotes kidney function
FALSE
Denominator denotes LUNG function
TRUE or FALSE
Respiratory acidosis- decrease in pH
TRUE
TRUE or FALSE
Respiratory alkalosis- increase in pH
TRUE
TRUE or FALSE
Nonrespiratory acidosis- decrease in pH
TRUE
TRUE or FALSE
Nonrespiratory alkalosis- increase in pH
TRUE
TRUE or FALSE
Respiratory acidosis- increase in pH
FALSE
Respiratory ALKALOSIS- increase in pH
TRUE or FALSE
Respiratory alkalosis-decrease in pH
FALSE
Respiratory alkalosis-increase in pH
What are the pH of buffer?
acidic buffer & alkaline buffer
TRUE or FALSE
Nonrespiratory acidosis-increase in pH
FALSE
Nonrespiratory acidosis-DECREASE in pH
TRUE or FALSE
Nonrespiratory alkalosis- decrease in pH
FALSE
Nonrespiratory alkalosis- INCREASE in pH