ACHEM Selecting Analytical Method Flashcards
Ratio for the change in amount of unit to the change in signal intensity. It is the measure of a methods ability to distinguish between two samples
Calibration sensitivity (m)
Lowest concentration or absolute amoujt of analyte that has a signal larger than the signal from the suitable blank
Detection limit
The number of analyses of similar types which have to be performed
To be Consider or not
To be consider
Defined as the fraction of the named material present in the stated chemical form
Purity
These aee acceptable food, drug or medicinal use, they also meets or EXCEEEDS requirements set by the National Formulary
NF national Fomulary
Problems arising from the nature of the target material including its radioactivity corrosiveness hygroscopicity etc is being considered un selecting analytical method
True
Have the highest purity chemical hrade with greater than or less than 95% and are strictly use for quality specifications
ACS american chemical society
Possible interferences, concentration range, availability of facilities, time required for analysis, and the number of analyses os same type is being considered in selecting analytical method
True
Possible interferences, concentration range, availability of facilities, time required for analysis, and the number of analyses os same type is being considered in selecting analytical method
True
Refer to how clear a signal is relative to how noisy these random fluctuations are also the magnitude of the background divided by the sd of the background signal
Signal to noise ratio
______ of an analytical method is ____ if its signal depends only on the analyte (one component only)
Specificity, specific
This meets or exceeds requirements set by the pharmacopeia and are accepted for food, drug, or medicinal use
US united states pharmacopeia
Determines the materials purity
Grade
1.Degree of sophistication
2.
3.
4.cost
5. Time requirements
Selectivity and sensitivity
Have relatively high quality with exact levels of impurities known often usedfor general work where Analytical Reagent is not required
Laboratory grade
State the 6 grades of chemicals
ACS
AR
USP
National formulary
Laboratory
Technical or commercial
This grade is for low grade applications for qualitative testing and often supplied in bulk having purity of less than 90%
Technical or commercial
This grade is for low grade applications for qualitative testing and often supplied in bulk having purity of less than 90%
Technical or commercial
The grade and purity of chemicals have siginificant effect in analysis that why it needs to be _______ with intended purpose
Consistent
Example of technical or commercial grade chemicals
Methanol
Sodium carbonate or baking soda
Example of technical or commercial grade chemicals
Methanol
Sodium carbonate or baking soda
3rd stanndarr where generally ang globally recognised standard employed by testing and calibration lab provides technical competence to perform particular tests
ISO/IEC 17025:2005
A protocol that requires all the organizations manufacturing products for human consumption to be safe pure and effective
GMP or cGMP
These serves as a means of evaluating the current performance of the method being used and this is desribed as a parameter to ensure the quality of individual or batch result of the laboratory activities
QC in Laboratories
Also known as check sample, Samples of known composition having similar matrix and analyte concentration to the samples to be analysed,
QC samples
It needs to be close as possible in composition to the samples but without the analyte present
Blank
Protocol required for all analytical lab which is concerned in overall process in lab from planning, execution, recording. A sebsection of GMP
GLP
Promofes adoption of a process approach when developing, implementing, and imporoving the effectiveness of a QMS
ISO 9000 QMS
Medical laboratory should abide
ISO 15189
This is known as fortification which involves adding a known quantity of analyte to a sample to test whether the response to a sample is the same as that of expected from calibration curve.
Spike
These are blank similat to a method blank where in sample containing all components except abalyte is taken through all steps of analytical procedures but also it has been exposed to site of sampling and aew subjected to all possible sources of contamination.
Field blank
These sanples provide less formal check than conventional QC samples and usually repeated later in the batch of samples or in different batch. This variation between is then studied and must be within the acceptable limit
Repeat sample
This samaples are not been subjected to all sample preparation procedure
Reagent blank
Samples inserted into analytical batch without knowledge of analyst
Blind samples
Method blank is that a sample containing _______ except analyte is taken through _____ of the process
All components, all steps
A good qc sample must have
1.Fully characterized in house or by third part
2. Similar in composition to the types of samples normally required
3. _____; homogenous; available in _____ quantities because they are repeatedly examined.
Stable, large
