ACAAI Review Book Ch 2: Cells in Immune Responses Flashcards
study table 2-3 Major CD4+ Families pg 62
see picture in notes
All but which of the following are true about natural Tregs?
A. CD25 (IL2-Ra) expression is inducible
B. specific markers include CD4, CD25, FOXP3
C. secrete IL-10 and TGFbeta
D. originate in the thymus
E. respond to thymically presented autologous antigens
A. CD25 (IL2-Ra) expression is inducible
it is CONSTITUITIVE
it is inducible on induced Tregs Tr1 and iTregs Th3
Tr1 cells DO NOT express FOXP3
Which of the following CD4+ families is incorrectly matched with what it is induced by, produces, and associated transcription factors?
A. Tfh : IL-6, IL-12 : IL-12 : Bcl-6 downregulates CCR7 and upregulates CXCR5
B. Th2 : IL-4, IL-25, IL-33, TLSP : IL-4, IL-5, IL-13 : GATA3, STAT6
C. Treg : TGFbeta, retinoic acid : IL-10, TBFbeta : FOXP3, sTAT5
D. Th1 : IL-12, IL-27, IL-18, IFNy : IL-2, IFNy, TNF : Tbet, STAT4, STAT1
E. Th17 : IL6, IL-1, IL-21, IL-23, TGFbeta : IL-17, IL-1, IL6 : RORyT, STAT3
A. Tfh : IL-6, IL-12 : IL-12 : Bcl-6 downregulates CCR7 and upregulates CXCR5
IL-21 NOT IL-12
T cells express CCR7 - binds CCL19/21 in parafollicular zone vs. what in B cells?
B cells express CXCR5 which bind CXCL13 in follicles
the correct order of recombination events is:
A. V joins J, D removed
B. D removed, V joins J
C. V joins D, J joins D, D removed
D. D joins J, V joins DJ
D. D joins J, V joins DJ
in Ig heavy chains and TCRs
in Ig light chains lacking D segment, V joins J for VJ recombination
see pg 65 for events in recombination and VDJ rearrangement
find better picture in JW
Put the events of lymphocyte gene rearrangement in order:
A. hairpin opening and end processing, joining, synapsis, cleavage
B. Joining, cleavage, synapsis, hairpin opening and end processing
C. cleavage, synapsis, hairpin opening and end processing, joining
D. synapsis, cleavage, hairpin opening and end processing, joining
D. synapsis, cleavage, hairpin opening and end processing, joining
which of the following adds nucleotides for junctional diversity, creating the greatest variable for diversity?
A. Rag-1/Rag-2 B. Terminal deoxynucleotidyl transferase C. endonuclease C. DNA Ligase IV: XRCC4 D. DNA-PK (DNA-dependent protein kinase complex) E. Artemis F. Ku
B. Terminal deoxynucleotidyl transferase (TdT)
Positive selection occurs at what stage of B cell development?
A. Mature naive
B. Immature naive
C. Pre-B cell
D. Pro-B cell
C. Pre-B cell
Negative selection occurs at what stage of B cell development?
A. Mature naive
B. Immature naive
C. Pre-B cell
D. Pro-B cell
B. Immature naive
gains IgM surface marker
loses CD43
Light chain rearrangement occurs at what stage of B cell development?
A. Mature naive
B. Immature naive
C. Pre-B cell
D. Pro-B cell
C. Pre-B cell
kappa or lambda
pre-BCR- u (mu) heavy chain with surrogate light chain, signals B cell to stop heavy chain rearrangement
Heavy chain rearrangement occurs at what stage of B cell development?
A. Mature naive
B. Immature naive
C. Pre-B cell
D. Pro-B cell
D. Pro-B cell
Alternative splicing occurs at what stage of B cell development?
A. Mature naive
B. Immature naive
C. Pre-B cell
D. Pro-B cell
A. Mature naive
- transmembrane and cytoplasmic exons are spiced out of primary mRNA transcript to determine whether immunoglobulin is membrane bound (or can be secreted)
gains IgD surface marker
express CXCR5 and recruited to LN follicles along CXCL13 gradient (CXCL1 produced by follicular DCs)
see more details on pg 67
No TACI is a CVID RF because it is a signal for what for B cells?
receptor for survival signals BLYSS, BAFF, APRIL
receptor is BCMA later on
true or false: thymic medullary epithelial cells express AIRE
true - involved in negative selection
positive selection is mediated by peptides bound to MHC on thymic cortical epithelial cells
table 2-8 pg 68 T cell development in the thymus table
All but which of the following type of dendritic cells express CD11c?
A. conventional
B. plasmacytoid
C. Langerhans
D. Interstitial
B. plasmacytoid
express CD123, lymphoid precursor (vs others are myeloid), secrete large amounts of IFNa w/ viral infections
Langerhans cells are marked by Bierbeck granules
see figure pg 70 on Macros details
These are class I MHC like molecules that present non-peptide (lipid and glycolipid) antigens to T cells, and are a marker on biopsy for Langerhans cell histiocytosis
CD1
specifically CD1a (also CD207)
see pages 76-77 for comparing mast cells, basophils, and eosinophils
know preformed mediators of each
the high affinity IgE recetptor FcERI contains what chains?
A. 2 alpha, 2 beta, 1 gamma B. 2 alpha, 1 beta, 1 gamma C. 1 alpha, 2 beta, 1 gamma D. 2 alpha, 2 beta, 2 gamma E. 1 alpha, 1 beta, 2 gamma
E. 1 alpha, 1 beta, 2 gamma
some CIU patients have antibodies directed against the alpha chain of the FcERI
pg 80- ways mast cells can be activated
what receptor is unique to basophils that is important for their differentiation and expansion?
CD123 (IL-3 receptor)
Which 2 of the following is more produced by mast cells are not found in basophils?
A. LTB4 B. tryptase C. chymase D. carboxypeptidase E. heparin G. PGD2
A. LTB4
G. PGD2
All but which of the following are preformed secondary /specific granules in eosinophils?
A. eosinophil cationic protein B. eosinophil derived neurotoxin C. major basic protein D. Charcot-Leyden crystals E. eosinophil peroxidase
D. Charcot-Leyden crystals (galectin-10)
these are in primary granules
eosinophils migrate to tissue in response to what?
RANTES (CCL-5) and eotaxin (CCL-11 eotaxin-1, CCL-24 eotaxin 2)
Which of the following is not associated with eosinophilia?
A. Isospora belli B. protozoa C. Sarcocystis D. strongyloidiasis E. hookworm, flukes, filiarisis F. Schistosomiasis
B. protozoa
not ALL protozoa, only A and C
