ABO Discrepancy

Question 1

What are the four (4) discrepancies for the extra reactions in the forward testing?

Resolutions?

Answer 1

1) Rouleaux
2) Cold Auto Abs
3.) Acquired B-like Ags
4.) Polyaggutination

1) Wash cells 4-6x
2) Warm saline wash 4-6x
3. Check pt. dx. for GI tract disease
4.) Use monoclonal reagents

Question 2

What are the six (6) discrepancies for the mixed field reactions in the forward testing?

Resolutions?

Answer 2

1) Recent transfusion with “out of group” blood
2) Bone marrow transplant
3) Exchange Transfusions
4) A3 or B3 Subgroups
5) Fetal-maternal bleeding
6) Chimeras

1-6) Check patient history

Question 3

What is the discrepancy for the weak/missing reactions in the forward testing?

Resolutions?

Answer 3

Subgroups of A or B

Test with A1 Lectin, A,B

Question 4

What is the three (3) discrepancy for the weak/missing reactions in the reverse testing?

Resolutions?

Answer 4

1. Age of patient
2. Disease state
3. True Chimera Twins

1-3) Increase serum to cell ratio, incubate at RT or 4C

Question 5

What are the three (3) discrepancies for the extra reactions in the reverse testing?

Resolutions?

Answer 5

1) Rouleaux
2) Auto Ab
3) Allo Ab

1. Saline Replacement
2. Pre-Warm Testing
3. 3+3 Rule