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Blood Bank CCMLS > ABO > Flashcards

ABO Flashcards

1
Q

Name the terminal sugar which defines the “A” blood group antigen

A

n-acetyl galactosamine

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2
Q

Name the terminal sugar which defines the “B” blood group antigen.

A

D-galactose

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3
Q

Name the sugar that serves as a “base for attachment” of other sugars which define blood group antigens.

A

Fucose

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4
Q

Bombay
RBCs
are different from normal
RBCs
because:

A

There was no inheritance of the H gene
There is no attachment of fucose to the precursor

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5
Q

What antibody(ies) are present in the serum of a Bombay individual?

A

Anti-A
Anti-B
Anti-H

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6
Q

Which of the genes below have a role in ABO antigen
formation?
H
A
Se

A

H
A

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7
Q

What phenotypes are possible in offspring if mother is group A and father is group AB?

A

A
B
AB

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8
Q

What genotypes are possible in offspring if mother is group AB and father is group O?

A

BO
AO

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9
Q

If one did ABO grouping by routine technique, what blood group would the Bombay individual appear to be?

A

Group O

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10
Q

What genotypes are possible in offspring if mother is group A and father is group AB?

A

AA
BO
AO
AB

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11
Q

What genotypes are possible in offspring if mother is group O and father is group A?

A

OO
AO

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12
Q

What phenotypes are possible in offspring if mother is group O and father is group A?

A

A
O

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13
Q

Which situations could cause mixed field agglutination when doing ABO testing?

A

A3 subgroup
A group A person who has been transfused with a large amount of group O blood

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14
Q

What phenotypes are possible in offspring if mother is group AB and father is group O?

A

A
B

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15
Q

What is the purpose of an
auto control when incubating reverse groupings at 4 degrees C?

A

To make sure that reactions are due to ABO antibodies and not cold autoantibodies

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16
Q

What is the appropriate action if one observes
rouleaux
when doing blood group testing?

A

Wash the cells and repeat testing

17
Q

Which of the following would cause unexpected positive reactions in the reverse group of ABO testing?

A

Presence of an unexpected IgM alloantibody
Presence of a cold alloantibody
A group A2 person with an anti-A1 antibody

18
Q

Why is it helpful to know the patient’s age when doing ABO testing?

A

Newborns have no antibodies of their own
As on ages, antibody strength may decline

19
Q

The following results were obtained on a cord blood:
RBC Testing:
RBCs+ Anti A : 0
RBCs+ Anti B : 2+
RBCs+ Anti A,B : 3+
Serum Testing: Serum + A1 Cells : 0
Serum + B Cells : 0
What is the person’s ABO group?

A

Type B

20
Q

The following results were obtained on an adult patient:
RBC Testing:
RBCs+ Anti A : 0
RBCs+ Anti B : 0
RBCs+ Anti A,B : 0
Serum Testing: Serum + A1 Cells : 0
Serum + B Cells : 0
What is the person’s ABO group?

A

Cannot interpret, should try cold enhancement of reverse group reactions

21
Q

When one detects a pattern of results like the following, what A subgroup is most likely:
RBC Testing:
RBCs+ Anti A : 1+mf
RBCs+ Anti B : 0
RBCs+ Anti A,B : 1+mf
Serum Testing:
Serum + A1 Cells : 0
Serum + B Cells : 3+

A

A3

22
Q

The following results were obtained on an adult patient:
RBC Testing:
RBCs+ Anti A : 3+
RBCs+ Anti B : 0
RBCs+ Anti A,B : 3+
Serum Testing:
Serum + A1 Cells : 1
Serum + B Cells : 4+
What is the person’s ABO group?

A

Cannot interpret, should test cells with anti-A1 lectin
Cannot interpret, should do antibody screen on serum

23
Q

You are doing ABO grouping on an adult patient who received an emergency transfusion three days ago with large volumes of group O blood for a gunshot wound. From previous records, the patient’s ABO group is B.
Your results today:
RBC Testing:
RBCs+ Anti A : 0
RBCs+ Anti B : 2+mf
RBCs+ Anti A,B : 2+mf
Serum Testing:
Serum + A1 Cells : 3
Serum + B Cells : 0
What is causing the weak reactions in the forward group?

A

The donor cells

24
Q

What is the purpose of an
O cell control
when incubating reverse groupings at 4 degrees C?

A

To make sure that reactions are due to ABO antibodies and not cold alloantibodies

25
Q

Which antibodies are normally present in the serum of a Bombay individual?

A

Anti-A
Anti-B
Anti-A,B
Anti-H

26
Q

order the blood groups from most H substance present to the least

A

O> A2> B> A2B> A1> A1B> Bombay

27
Q

A person with the blood type of ‘A’ will have which of the following antibodies in their serum

A

Anti-B

28
Q

A person with the blood type of ‘B’ will have which of the following antibodies in their serum

A

Anti-A

29
Q

A person with the blood type of ‘AB’ will have which of the following antibodies in their serum

A

none

30
Q

A person with the blood type of ‘O’ will have which of the following antibodies in their serum

A

Anti-A

Anti-B

31
Q

True or False:
Without inheriting the ‘H’ Gene, immunodominant sugars cannot be created

A

true

32
Q

True or False:
‘A’ and ‘B’ genes code for enzymes that replace the ‘H’ antigen

A

true

33
Q

Put the following blood types in order from least ‘H’ antigen present to most ‘H’ antigen present

B
O
A1
A2
A1B
A2B

A

A1B
A1
A2B
B
A2
O

34
Q

If a patient has the following genes, which antigens would appear in their secretions:
Se/se | H/h | AB

A

H antigen

A antigen

B antigen

35
Q

If a patient has the following genes, which antigens would appear in their secretions:
se/se | H/h | AB

A

none

36
Q

If a patient has the following genes, which antigens would appear in their secretions:
Se/Se | H/h | O

A

H antigen

37
Q

If a patient has the following genes, which antigens would appear in their secretions:
Se/Se | h/h | A

A

none

38
Q

If a patient has the following genes, which antigens would appear in their secretions:
Se/Se | H/h | A

A

H antigen

A antigen

Blood Bank CCMLS (7 decks)

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