A3 Upskilling Pipetting

Question 1

are devices that aid in the transfer of samples that can either be manually or electronically operated

Answer 1

Pipettes

Question 2

A serological pipette’s volumes are in the milliliter range (1-50 ml or more) and are commonly used in protein work or cell cultures

Question 3

The __________ consists of a mechanical-driven system that allows control
of fluid entering the pipette.

Answer 3

syringe-style pipette pump

Question 4

is a cylindrical rod that fits snuggly on the body.

Answer 4

plunger or piston

Question 5

is responsible for moving the plunger, allowing the user to manipulate the amount of liquid that enters or exits the pipette.

Answer 5

dial or wheel

Question 6

relieves the pump of the pressure by allowing air to enter the body and the drawn liquid to be dispensed

Answer 6

exhaust valve

Question 7

is a rubber ring located at the bottom part of the pump and holds the serological or glass pipettes in place during the entire process.

Answer 7

gasket

Question 8

holds all these components and is where the user can grip the pump during use.

Answer 8

Body

Question 9

Parts of a micropipette

Answer 9

Tip holder
Tip ejector collar
Volume display
Body
Tip ejector
Grip
Plunger & Volume adjustment knowb

Question 10

is a movable rod that, when pushed, controls the aspiration of the liquid.

Answer 10

Plunger

Question 11

This knob is used to change the volume of the liquid to be pipetted to the desired amount.

Answer 11

volume adjustment knob

Question 12

holds the replaceable tips that would hold the liquid to be transferred.

Answer 12

Tip holder

Question 13

When done using the tips, it can be removed by pressing the _______, to which the tip _________will push down the tip to be released.

Answer 13

tip ejector; ejector collar

Question 14

houses all the internal components of the pipettor and is used in holding the device.

Answer 14

Body

Question 15

hold the micropipette securely
while working and to hang it up in pipette rack stands when not in use.

Answer 15

Grip

Question 16

various types of pipette tips:

Answer 16

Standard tips (ABC) = multipurpose
white (A) for P0.1-10
yellow (B) for P20-200
blue (C) for P1000

barriers and filters (DEG) = cross-contamination control
Wide orifice tips (E) can
transfer macromolecules, viscous materials, or cells without damaging them
Gel loading tips (G)

Extended tips (F) = containers are deep

Sample loss during pipetting is also reduced using low-retention tips